Chlorophyll Fluorescence Video Imaging: A Versatile Tool for Identifying Factors Related to Photosynthesis

Rühle, Thilo; Reiter, Bennet; Leister, Dario

doi:10.3389/fpls.2018.00055

Cited by 23 publications

(14 citation statements)

References 148 publications

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“…One of the most reliable markers of this process is decreased photosystem II (PS II) activity [98]. Both relative chlorophyll content and PS II efficiency can be easily quantified with pulse amplitude modulation (PAM) fluorometry [99,100]. Thereby, the ratio of minimum (background) and potentially maximum chlorophyll fluorescence (Fv/Fm) is interpreted as the maximum of PS II photochemical activity and might be considered as a reliable marker of PS II photoinhibition and one of the most important indicators of drought stress [101].…”

Section: Physiological and Biochemical Characterization Of Droughtmentioning

confidence: 99%

Methodology of Drought Stress Research: Experimental Setup and Physiological Characterization

Osmolovskaya

Shumilina

Kim

et al. 2018

IJMS

179

121

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Drought is one of the major stress factors affecting the growth and development of plants. In this context, drought-related losses of crop plant productivity impede sustainable agriculture all over the world. In general, plants respond to water deficits by multiple physiological and metabolic adaptations at the molecular, cellular, and organism levels. To understand the underlying mechanisms of drought tolerance, adequate stress models and arrays of reliable stress markers are required. Therefore, in this review we comprehensively address currently available models of drought stress, based on culturing plants in soil, hydroponically, or in agar culture, and critically discuss advantages and limitations of each design. We also address the methodology of drought stress characterization and discuss it in the context of real experimental approaches. Further, we highlight the trends of methodological developments in drought stress research, i.e., complementing conventional tests with quantification of phytohormones and reactive oxygen species (ROS), measuring antioxidant enzyme activities, and comprehensively profiling transcriptome, proteome, and metabolome.

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Section: Physiological and Biochemical Characterization Of Droughtmentioning

confidence: 99%

Methodology of Drought Stress Research: Experimental Setup and Physiological Characterization

Osmolovskaya

Shumilina

Kim

et al. 2018

IJMS

179

121

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“…The invention of video imaging systems was an important breakthrough in the field of chlorophyll fluorescence analysis ( Omasa et al, 1987 ; Fenton and Crofts, 1990 ). In fact, this technique not only allowed to spatially examine the heterogeneity within a sample, but it also made possible to evaluate those changes simultaneously on a wide number of samples, making the CFI more popular as diagnostic ( Baker, 2008 ), screening ( Barbagallo et al, 2003 ) and phenotyping tool ( Rühle et al, 2018 ; Pérez-Bueno et al, 2019 ).…”

Section: Tools For Imaging Chlorophyll Fluorescencementioning

confidence: 99%

Imaging of Chlorophyll a Fluorescence in Natural Compound-Induced Stress Detection

et al. 2020

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Imaging of chlorophyll a fluorescence (CFI) represents an easy, precise, fast and non-invasive technique that can be successfully used for discriminating plant response to phytotoxic stress with reproducible results and without damaging the plants. The spatio-temporal analyses of the fluorescence images can give information about damage evolution, secondary effects and plant defense response. In the last years, some studies about plant natural compounds-induced phytotoxicity have introduced imaging techniques to measure fluorescence, although the analysis of the image as a whole is often missed. In this paper we, therefore, evaluated the advantages of monitoring fluorescence images, presenting the physiological interpretation of different possible combinations of the most relevant parameters linked to fluorescence emission and the images obtained.

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“…Він характеризує період теплової адаптації листка. При малому значенні періоду адаптації перехід має більшу крутість, а при великих значеннях він більш повільний (Rühle et al, 2018). Параметр F m вказує на найвищий рівень флуоресценції, який реєструється на індукційній кривій у вигляді максимуму.…”

Section: результати та їх обговоренняunclassified

Assessment of the impact of horse-chestnut leaf miner (Cameraria ohridella Deschka & Dimič, 1986) on photosynthesis of common horse chestnut (Aesculus hippocastanum Linnaeus, 1753)

Holoborodko

Алексеева

Seliutina

et al. 2020

ean

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The article presents the results of studies on Cameraria ohridella Deschka & Dimič, 1986 feeding on the state of the photosynthetic apparatus in Aesculus hippocastanum Linnaeus, 1753. The studies were carried out in June 2019 (during the life of C. ohridella first generation) on the territory of the Botanical Garden of the Oles Honchar Dnipro National University. Photosynthesis is one of the processes most susceptible to stress factors; important information on state of the photosynthetic apparatus in plants under influence of various abiotic and biotic factors can be obtained by fluorescence analysis. Chlorophyll fluorescence is an indicator that allow to investigate a behavior of photochemical reactions related to system II (PSII) in living objects as an indicator the most sensitive to effect of environmental factors. One of the effective methods for plant state monitoring is a technique on chlorophyll fluorescence induction (CFI) measurement, which at the present stage can be introduced due to the use of biosensors. The objective of our study was to establish characteristics of C. ohridella vital activity on the photosynthesis process in A. hippocastanum trees using CFI evaluation technique. To diagnose photosynthesis violation of native chlorophyll in a living leaf of A. hippocastanum, we used a portable fluorometer “Floratest” (spectral range for measuring fluorescence intensity 670 to 770 nm) developed by V. M. Glushkov Institute of Cybernetics of National Academy of Sciences of Ukraine. The studies were conducted using alive A. hippocastanum leaves not damaged by C. ohridella and, accordingly, the damaged ones. Analysis of the data obtained showed that the worm feeding affects all CFI parameters (initial CFI value (non-damaged leaf: 582 relative units, damaged: 264); “plateau” CFI value (1460 to 722); maximum CFI (1890 to 940) and stationary CFI value after light adaptation of the leaf (1530 to 746)). Our studies have shown that CFI technique using “Floratest” apparatus allow express determining the general condition of the plant by assessing the main process of vital activity, namely photosynthesis. Analysis of chlorophyll fluorescence parameters is a powerful and effective tool for determining the phytophage effect on the plant.

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Chlorophyll Fluorescence Video Imaging: A Versatile Tool for Identifying Factors Related to Photosynthesis

Cited by 23 publications

References 148 publications

Methodology of Drought Stress Research: Experimental Setup and Physiological Characterization

Methodology of Drought Stress Research: Experimental Setup and Physiological Characterization

Imaging of Chlorophyll a Fluorescence in Natural Compound-Induced Stress Detection

Assessment of the impact of horse-chestnut leaf miner (Cameraria ohridella Deschka & Dimič, 1986) on photosynthesis of common horse chestnut (Aesculus hippocastanum Linnaeus, 1753)

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