Chlorophyll interference in microalgal lipid quantification through the Bligh and Dyer method

Archanaa, S.; Moise, Sandhya; Suraishkumar, G. K.

doi:10.1016/j.biombioe.2012.07.002

Cited by 32 publications

(15 citation statements)

References 19 publications

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“…Stationary phase UVA‐treated samples from Day 13 were taken for acid catalyzed transesterification. Prior to transesterification, the chlorophyll in the samples was extracted using dimethyl sulfoxide (DMSO), to prevent chlorophyll interference in the fatty acid analysis . Transesterification was done using 150 mg of the dry biomass (after DMSO extraction and lyophilization) according to Das et al Fatty acid methyl esters (FAMEs) were extracted in hexane:chloroform (4:1, v/v) mixture and subsequently analyzed in a gas chromatography–mass spectrometry (GC‐MS, Clarus 600S; Perkin Elmer, CT).…”

Section: Methodsmentioning

confidence: 99%

“…Prior to transesterification, the chlorophyll in the samples was extracted using dimethyl sulfoxide (DMSO), to prevent chlorophyll interference in the fatty acid analysis. 30 Transesterification was done using 150 mg of the dry biomass (after DMSO In addition to the entire growth period (15 days), three phase of growth were considered over the 15 day growth cycle viz. early log (Days 1-5), mid log (Days 6-10), and stationary (Days 11-15).…”

Section: Fatty Acid Profile Analysis By Gas Chromatography-mass Spectmentioning

confidence: 99%

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Simultaneous increases in specific growth rate and specific lipid content of Chlorella vulgaris through UV‐induced reactive species

Balan

Suraishkumar

2013

Biotechnology Progress

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A challenge in algae-based bio-oil production is to simultaneously enhance specific growth rates and specific lipid content. We have demonstrated simultaneous increases in both the above in Chlorella vulgaris through reactive species (RS) induced under ultraviolet (UV) A and UVB light treatments. We postulated that the changes in photosystem (PS) stoichiometry and antenna size were responsible for the increases in specific growth rate. UVB treatment excited PSII, which resulted in a twofold to sevenfold increase in PSII/PSI ratio compared to control. An excited PSII caused a 2.7-fold increase in the specific levels of superoxide and a twofold increase in the specific levels of hydroxyl radicals. We have established that the increased specific intracellular RS (si-RS) levels increased the PSII antenna size by a significant 10-fold as compared to control. In addition, the 8.2-fold increase in specific lipid content was directly related to the si-RS levels. We have also demonstrated that the RS induced under UVA treatment led to a 3.2-fold increase in the saturated to unsaturated fatty acid ratio. Based on the findings, we have proposed and demonstrated a UV-based strategy, which achieved an 8.8-fold increase in volumetric lipid productivity.

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Section: Methodsmentioning

confidence: 99%

Section: Fatty Acid Profile Analysis By Gas Chromatography-mass Spectmentioning

confidence: 99%

Simultaneous increases in specific growth rate and specific lipid content of Chlorella vulgaris through UV‐induced reactive species

Balan

Suraishkumar

2013

Biotechnology Progress

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“…Even for the same microalgal species, the excitation and emission wavelengths can be quite different [11,14,18,23,24]. This difference might be caused by the diversity of algal strains and their fatty acid compositions.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 97%

“…Lipids were extracted from the dried algal cells (100 mg or more) in accordance with the previously described method [19,23,35]. The same process was repeated three times for the complete extraction of the lipids from the algae.…”

Section: Gravimetric Determinationmentioning

confidence: 99%

Improved Nile red staining of Scenedesmus sp. by combining ultrasonic treatment and three-dimensional excitation emission matrix fluorescence spectroscopy

et al. 2015

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“…Posteriormente se realiza una filtración y una centrifugación; después de la cual se separan las fases de metanol y cloroformo, y se remueve el cloroformo por evaporación [14]. La Fig.…”

Section: E Extracción Y Caracterización De Aceitesunclassified

Caracterización y perfil lipídico de aceites de microalgas

et al. 2015

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<p>El uso creciente del biodiésel ha impulsado la búsqueda de nuevas materias primas, dentro de las que se destacan las microalgas. En esta investigación se estudió el cultivo de las microalgas Chlorella sp. y Dunaliella salina bajo diferentes condiciones de pH y concentración de nitrógeno (mgL-1), y la caracterización de los aceites obtenidos, con el fin de evaluar su potencial uso como materia prima en la producción de biodiésel. La D. salina alcanzó una máxima concentración celular de 1.15x106 células mL-1 en 6 días de cultivo en unas condiciones de 8.5 pH y 0.1 mg L-1 concentración de nitrógeno, mientras que Chlorella sp. presentó una máxima concentración celular de 2.6x107 células mL-1 en 14 días de cultivo en unas condiciones de 7.5 pH y 0.1 mg L-1 concentración de nitrógeno. La extracción de aceite intracelular de las microalgas cultivadas bajo las mejores condiciones de crecimiento celular se realizó empleando el método modificado de Bligh & Dyer. Al determinar el perfil de ácidos grasos de los aceites extraídos de ambas microalgas, se encontraron en mayor proporción dos ácidos grasos insaturados: ácido linolénico y ácido oleico. En el aceite de D. salina se encontró una concentración de 51% p/p de ácido linolénico, mientras que para el aceite de Chlorella sp. fue de 39% p/p. Con relación al ácido oleico, el aceite de Chrorella sp. presentó una concentración de 35% p/p, superior al aceite de D. salina, con 25% p/p.</p>

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Chlorophyll interference in microalgal lipid quantification through the Bligh and Dyer method

Cited by 32 publications

References 19 publications

Simultaneous increases in specific growth rate and specific lipid content of Chlorella vulgaris through UV‐induced reactive species

Simultaneous increases in specific growth rate and specific lipid content of Chlorella vulgaris through UV‐induced reactive species

Improved Nile red staining of Scenedesmus sp. by combining ultrasonic treatment and three-dimensional excitation emission matrix fluorescence spectroscopy

Caracterización y perfil lipídico de aceites de microalgas

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