Chloroplast avoidance movement as a sensitive indicator of relative water content during leaf desiccation in the dark

Nauš, Jan; Šmecko, Slavomír; Špundová, Martina

doi:10.1007/s11120-016-0291-5

Cited by 15 publications

(12 citation statements)

References 44 publications

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“…Recently, it was observed that blue-light-induced chloroplast movements are very sensitive to drought stress. Inhibition occurred already at RWC values considerably above 70%, and was, therefore proposed to be a sensitive tool for small changes in RWC (Nauš et al 2016). In typical C3 plants, critical leaf relative water content is about 70% and below this value non-stomatal effects occur.…”

Section: Question 25: What Do Chl a Fluorescence Measurements Tell Usmentioning

confidence: 99%

Frequently asked questions about chlorophyll fluorescence, the sequel

Schansker²,

et al. 2016

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Using chlorophyll (Chl) a fluorescence many aspects of the photosynthetic apparatus can be studied, both in vitro and, noninvasively, in vivo. Complementary techniques can help to interpret changes in the Chl a fluorescence kinetics. Kalaji et al. (Photosynth Res 122:121–158, 2014a) addressed several questions about instruments, methods and applications based on Chl a fluorescence. Here, additional Chl a fluorescence-related topics are discussed again in a question and answer format. Examples are the effect of connectivity on photochemical quenching, the correction of F V/F M values for PSI fluorescence, the energy partitioning concept, the interpretation of the complementary area, probing the donor side of PSII, the assignment of bands of 77 K fluorescence emission spectra to fluorescence emitters, the relationship between prompt and delayed fluorescence, potential problems when sampling tree canopies, the use of fluorescence parameters in QTL studies, the use of Chl a fluorescence in biosensor applications and the application of neural network approaches for the analysis of fluorescence measurements. The answers draw on knowledge from different Chl a fluorescence analysis domains, yielding in several cases new insights.

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Section: Question 25: What Do Chl a Fluorescence Measurements Tell Usmentioning

confidence: 99%

Frequently asked questions about chlorophyll fluorescence, the sequel

Schansker²,

et al. 2016

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“…weighed again to obtain the turgid mass (Mt) and placed in a forced ventilation oven at 75°C until constant dry mass (Md). From these variables, the RWC was calculated as [RWC = ((Mf-Md) / (Mt-Md)) x100] (Nauš et al, 2016).…”

Section: Water Relationsmentioning

confidence: 99%

Morphophysiological changes in young plants of Jatropha curcas L. (Euphorbiaceae) subjected to water stress and recovery

Oliveira¹,

Silva²,

Santana³

et al. 2016

Afr. J. Agric. Res.

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To investigate drought-induced changes in morphophysiological characteristics, seedlings of two genotypes of Jatropha curcas (CNPAE 183 and CNPAE 191) were grown under two watering regimes: irrigated (-33.1 to 13.5 to kPa) and water deficit (-409.5 to 49.5 to kPa) for 55 days, followed by six days of rehydration (DAR). Withholding water led to a significant reduction (p<0.05) of leaf water potential (Ψ W ) and an increase in relative water content (RWC). The values of net photosynthetic rate (P N ), stomatal conductance to water vapor (g S ) and transpiration (E) were significantly (p<0.05) reduced 21 and 34 days after starting treatment (DAST) in plants of genotypes CNPAE 183 and CNPAE 191, respectively. After 6 DAR, only CNPAE 191 achieved a recovery of P N and E. Moreover, significantly (p<0.05) lower g S was measured in recovering plants of both genotypes, as compared to the controls. Drought stress led to reductions of 57 and 65% in whole-plant hydraulic conductance (K L ) in genotypes CNPAE 183 and CNPAE 191, respectively. Full recovery of K L was observed after 6 DAR. The average water consumption was 18% lower in plants subjected to water shortage, as compared to irrigated plants. However, drought-induced reduction in growth led to lower biomass water use efficiency (WUE biomass ) in plants subjected to water deficit. The effect of water stress was more intense in CNPAE 183 than in CNPAE 191, regarding the growth variables (leaf area, height and diameter), dry mass and root volume. Moreover, a delay in the effect of water stress in genotype CNPAE 191 was also observed, which suggests a higher tolerance of this genotype as compared to CNPAE 183. Altogether, the results showed strong drought-induced stomatal limitation of carbon assimilation and growth in J. curcas. Slight genotypic differences were detected, CNPAE 191 being less sensitive to the imposed experimental conditions than CNPAE 183.

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“…To explain these contradictory results we hypothesize that fluence rate during growth may affect the chloroplast relocation system in barley. The plants that did not show chloroplast movements (Maai et al 2011 ) were grown at PAR 4–5 times stronger than those in which the movements took place (Nauš et al 2016 and the present work). This explanation needs to be carefully validated as no correlation was found between excess light tolerance and the extent of chloroplast moments in several monocot and dicot species (Königer and Bollinger 2012 ).…”

Section: Discussionmentioning

confidence: 50%

“…In line with its role as a stress hormone, ABA appears to enhance plant sensitivity to light and promote the chloroplast avoidance response (Eckstein et al 2016 ). While Maai et al ( 2011 ) were unable to show chloroplast redistribution in barley ( H. vulgare ), avoidance movement in this species was reported 5 years later (Nauš et al 2016 ). According to our results both accumulation and avoidance responses are conspicuous in barley and in other C3 crop species that we have analyzed.…”

Section: Discussionmentioning

confidence: 98%

“…As a consequence, changes in the position of chloroplasts can limit the rate of photosynthesis. Recently, it has been suggested that the process of chloroplast movements is sensitive to the relative water status of the cell (Nauš et al 2016 ). Although a crosstalk between chloroplast movements and drought stress is an interesting possibility, this idea needs further investigation.…”

Section: Introductionmentioning

confidence: 99%

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Chloroplasts in C3 grasses move in response to blue-light

2020

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Key message Brachypodium distachyonis a good model for studying chloropla st movements in the crop plants, wheat, rye and barley. The movements are activated only by blue light, similar to Arabidopsis. Abstract Chloroplast translocations are ubiquitous in photosynthetic organisms. On the one hand, they serve to optimize energy capture under limiting light, on the other hand, they minimize potential photodamage to the photosynthetic apparatus in excess light. In higher plants chloroplast movements are mediated by phototropins (phots), blue light receptors that also control other light acclimation responses. So far, Arabidopsis thaliana has been the main model for studying the mechanism of blue light signaling to chloroplast translocations in terrestrial plants. Here, we propose Brachypodium distachyon as a model in research into chloroplast movements in C3 cereals. Brachypodium chloroplasts respond to light in a similar way to those in Arabidopsis. The amino acid sequence of Brachypodium PHOT1 is 79.3% identical, and that of PHOT2 is 73.6% identical to the sequence of the corresponding phototropin in Arabidopsis. Both phototropin1 and 2 are expressed in Brachypodium, as shown using quantitative real-time PCR. Intriguingly, the light-expression pattern of BradiPHOT1 and BradiPHOT2 is the opposite of that for Arabidopsis phototropins, suggesting potential unique light signaling in C3 grasses. To investigate if Brachypodium is a good model for studying grass chloroplast movements we analyzed these movements in the leaves of three C3 crop grasses, namely wheat, rye and barley. Similarly to Brachypodium, chloroplasts only respond to blue light in all these species.

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Chloroplast avoidance movement as a sensitive indicator of relative water content during leaf desiccation in the dark

Cited by 15 publications

References 44 publications

Frequently asked questions about chlorophyll fluorescence, the sequel

Frequently asked questions about chlorophyll fluorescence, the sequel

Morphophysiological changes in young plants of Jatropha curcas L. (Euphorbiaceae) subjected to water stress and recovery

Chloroplasts in C3 grasses move in response to blue-light

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