Chloroplast DNA codes for the primary structure of the large subunit of Fraction I protein

Peptide mapping of the ribulose bisphosphate carboxylase large subunit from the genus Oenothera

“…Amino acid sequencing of the two types of subunit of RuBPCase provides such markers (23,28), but often other characteristics of the polypeptides which reflect their sequence can be used. Such studies employing isoelectric focusing (17,20) and peptide mapping (3,13) of the enzyme from the genus Nicotiana have shown that the large subunit (MW 55,000) is maternally inherited whereas the small subunit (MW 12,000-15,000) is inherited in a Mendelian fashion. The gene coding for the large subunit has been shown to be localized on chloroplast DNA in maize (6) and Chlamydomonas (9).…”

Synthesis of the small subunit of ribulose 1, 5‐diphosphate carboxylase on cytoplasmic ribosomes from greening bean leaves

“…The synthesis of the small subunit is therefore spatially separated from the synthesis of the large subunit by the chloroplast membrane. The genes for the two subunits are also separated in the cell; nuclear DNA coding for the structure of the small subunit [10] and chloroplast DNA coding for the large subunit [ 11 ]. The control mechanisms necessary to ensure stoichlometric production of the subunits are therefore of great interest and it is hoped that future experiments with the in vitro system for the synthesis of the small subunit will give some insight into possible control mechanisms.…”

Ribulose 1,5-diphosphate carboxylase from Oenothera. Purification and a peptide mapping procedure for the subunits

“…Previous reports of the numbers of tryptic peptides obtained by fingerprint analysis from the two subunits of several plant species show considerable variation which to some extent seems dependant upon the technique used and the interpretation of the resultant map. For the small subunit 9 (19), 13-16 (19,20,21,27), 23-28 (13,22,39,40) and 32 (24) peptides have been reported, whereas for the large subunit 19-27 (3,19,20,22,27), 42-43 (3,40) and 55 (24) peptides have been reported. In all cases peptides were identified as material which was stained with ninhydrin.…”

“…The enzyme from a number of species has been shown to have a molecular weight of about 550,000 and can be dissociated into two types of subunit (8,18); the larger of about 55,000 MW contains the catalytic site (28) and the smaller of about 13,000 MW which may have a regulatory function (29). Isoelectric focusing (23,36) and peptide mapping (3,21) studies of the subunits from some Nicotiana species have shown that the large subunit is maternally inherited, possibly coded by chloroplast DNA and i~hat the small subunit is inherited in a Mendelian manner characteristic of a nuclear DNA product. The large subunit has been shown to be synthesized from chloroplast mRNA (12,35) by chloroplast ribosomes (2), whilst the small subunit is probably synthesized on cytoplasmic ribosomes (11,33).…”