Chlortetracycline-Functionalized Silver Nanoparticles as a Colorimetric Probe for Aminoglycosides: Ultrasensitive Determination of Kanamycin and Streptomycin

Abstract

Aminoglycosides (AMGs) have been extensively used to treat infectious diseases caused by Gram-negative bacteria in livestock and humans. A selective and sensitive colorimetric probe for the determination of streptomycin and kanamycin was proposed based on chlortetracycline-coated silver nanoparticles (AgNPs–CTC) as the sensing element. Almost all of the tested aminoglycoside antibiotics can rapidly induce the aggregation of AgNPs, along with a color change from yellow to orange/red. The selective detection of aminoglycoside antibiotics, including tobramycin, streptomycin, amikacin, gentamicin, neomycin, and kanamycin, with other types of antibiotics, can be achieved by ultraviolet (UV) spectroscopy. This developed colorimetric assay has ability to detect various AMGs using in-depth surface plasmon resonance (SPR) studies. With this determination of streptomycin and kanamycin was achieved at the picomolar level (pM) by using a UV–visible spectrophotometer. Under aqueous conditions, the linear range of the colorimetric sensor for streptomycin and kanamycin was 1000–1,1000 and 120–480 pM, respectively. The corresponding limit of detection was 2000 pM and 120 pM, respectively. Thus, the validated dual colorimetric and ratiometric method can find various analytical applications for the ultrasensitive and rapid detection of AMG antibiotics in water samples.

Exploiting Fruit Waste Grape Pomace for Silver Nanoparticles Synthesis, Assessing Their Antioxidant, Antidiabetic Potential and Antibacterial Activity Against Human Pathogens: A Novel Approach

“…This may be because of its versatile applications in numerous prominent areas such as life sciences, material sciences, biomedical engineering, wastewater treatment, biosensors development, chemistry, and physics [ 1 ]. Silver nanoparticles (AgNPs) are well recognized metal nanoparticles (NPs) because of its exceptional biocompatibility and have demonstrated numerous properties such as antimicrobial, wound healing, biosensors, drug delivery agents, in biomedical devices, and as a catalyst [ 2 , 3 , 4 , 5 ]. Conversely, application of AgNPs in various sectors leads to environmental pollution and showed toxicity to the ecosystem and human health [ 6 ].…”

“…Among which, utilization of plant- or fruit waste-based extracts could be more beneficial due to simple, cost-effective, and eco-accommodating process relative to other biological routes of NPs synthesis [ 8 , 9 ]. There are a few reports of green synthesis of silver nanoparticles using leaf extract of Holoptelea integrifolia , Punica granatum , waste fruit extract of Vitis vinifera , and using single biomolecules such as gallic acid, chlorotetracycline, and lignin [ 1 , 2 , 3 , 4 , 5 , 8 , 9 , 10 , 11 , 12 , 13 ]. The synthesized AgNPs have been studied for various biogenic activities which increases its potential applications in biomedical, environment sector.…”

An Overview of Different Methods for Aminoglycoside Residue Determination

“…These articles show that kanamycin could be evaluated with gold nanoparticle at very low levels [70]. In another study chlortetracycline-coated silver nanoparticles-(UV) spectroscopy detect kanamycin in aqueous solution with LOD values in 120-480 picomolar [71].…”

Recent Advances in Optical Detection of Aminoglycosides

“…Other metal NPs include gallic acid-coated Ag NPs, methyl gallate conjugated Ag NPs, sodium dodecyl sulfate (SDS)-coated Ag NPs, and chlortetracycline-functionalized Ag NPs, chitosan-coated Au NPs, etc. These Ag NPs have been applied to the colorimetric detection of streptomycin [60,61], gentamicin [62], tobramycin [63], and kanamycin [64][65][66].…”