1997
DOI: 10.1016/s0006-3495(97)78900-8
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Cholesterol in condensed and fluid phosphatidylcholine monolayers studied by epifluorescence microscopy

Abstract: Epifluorescence microscopy was used to investigate the effect of cholesterol on monolayers of dipalmitoylphosphatidylcholine (DPPC) and 1 -palmitoyl-2-oleoyl phosphatidylcholine (POPC) at 21 +/- 2 degrees C using 1 mol% 1-palmitoyl-2-[12-[(7-nitro-2-1, 3-benzoxadizole-4-yl)amino]dodecanoyl]phosphatidylcholine (NBD-PC) as a fluorophore. Up to 30 mol% cholesterol in DPPC monolayers decreased the amounts of probe-excluded liquid-condensed (LC) phase at all surface pressures (pi), but did not effect the monolayers… Show more

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Cited by 103 publications
(110 citation statements)
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“…It remains in the solid condensed phase up to the point of monolayer collapse [46]. As can be observed from Fig.…”
Section: Dependence Of η and ε On The Film Pressurementioning
confidence: 56%
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“…It remains in the solid condensed phase up to the point of monolayer collapse [46]. As can be observed from Fig.…”
Section: Dependence Of η and ε On The Film Pressurementioning
confidence: 56%
“…The isotherm of cholesterol shows a single phase with a collapse pressure of approximately 45 mN m −1 , at which point the molecular area is ∼39Å 2 [15,46,49]. It remains in the solid condensed phase up to the point of monolayer collapse [46].…”
Section: Dependence Of η and ε On The Film Pressurementioning
confidence: 99%
See 1 more Smart Citation
“…Glassware used were washed by perchloric acid followed by rinsing with ddH 2 O and were dried for 2 hrs. at 180 to remove any organic and surface active impurities 23 .…”
Section: Methodsmentioning
confidence: 99%
“…The Langmuir monolayer, a model system that mimics a single leaflet of the bilayer, has been extensively used to investigate lateral lipid-lipid interactions, as well as the impact of antimicrobial peptide on lipid packing (Fischer et al 1984;Worthman et al 1997;Eeman et al 2006;Neville et al 2006;Bouffioux et al 2007;Dennison et al 2008;Wilke 2014). The main advantage of this system is that the molecular density and lipid composition can be varied without limitation, while the pressure-area isotherms are monitored by simultaneous visualization of the monolayer with microscopy techniques (Lösche et al 1983;Henon and Meunier 1991;Möhwald 1995;Kaganer et al 1999;Lheveder et al 2000).…”
Section: Charge Effect On Lipid Packing Perturbation Induced By Peptimentioning
confidence: 99%