Cholesterol in Serum

Abell, Liese L.; Levy, Betty B.; Brodie, Bernard B.; Kendall, Forrest E.; Kaplan, Ariel; Jacques, S.; Reinhold, John G.

doi:10.1016/b978-1-4831-9683-1.50008-3

Cited by 60 publications

(20 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The last method was primarily used to investigate the accuracy of the other tests. In an earlier report we found, in accordance with Attain et al (12), that the procedure of A bell et al (13) often recommended for reference purposes, leads to cholesterol values which are approximately 10% higher than those determined with enzymatic methods.…”

Section: Introductionsupporting

confidence: 72%

Comparison of 9 Methods for the Determination of Cholesterol

Haeckel¹,

Sonntag²,

Külpmann³

et al. 1979

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Summary: Seven enzymatic procedures for the determination of cholesterol in serum were compared with the Liebermann-Burchard-and a gas-chromatographic method. Using a decision matrix all methods could be ranked according to reliability and practicability. With the exception of the cholesterol oxidase-coupled Kageyama principle and the Liebermann-Burchard procedure, all the other methods showed similar reliability. Vergleich von 9 Methoden zur Bestimmung von CholesterinZusammenfassung: Sieben enzymatische Verfahren zur Bestimmung der Cholesterin-Konzentration im Serum wurden mit der Liebermann-Burchard-und einer gaschromatographischen Methode verglichen. Mit Hilfe einer Entscheidungsmatrix wurde eine Wichtung von Ergebnissen und Praktikabilität versucht. Dabei zeigt sich, daß sich mit Ausnahme des mit Cholesterinoxidase gekoppelten Kageyama-und des Liebermann-Burchard-Prinzips die übrigen Methoden etwa gleich zuverlässig verhielten.

show abstract

Section: Introductionsupporting

confidence: 72%

Comparison of 9 Methods for the Determination of Cholesterol

Haeckel¹,

Sonntag²,

Külpmann³

et al. 1979

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…Serum concentrations of total cholesterol and triglycerides were determined by Trinder's enzyme colorimetric method (Abell, Levy & Kendall, 1958;Trinder, 1969). HDL cholesterol was assayed by direct method (Sugiuchi, 1995).…”

Section: Determination Of Biochemical Parametersmentioning

confidence: 99%

Effects of Palm Oil Consumption on Lipid Profile among Rural Ivorian Youth

Alexandre¹,

Absalome²,

Edjème-Aké³

et al. 2017

JFR

View full text Add to dashboard Cite

As palm oil has been qualified as atherogen, we have studied the impact of its consumption on changes of lipid and lipoprotein profiles of young Ivorian healthy subjects living in rural areas. It is a descriptive cross-sectional analytical study of about 120 Ivorian subjects aged 18 to 30 years, including 65 regular consumers of palm oil and 55 subjects consuming that oil periodically as control subjects. Serum concentrations of triglycerides, total cholesterol, HDL, LDL cholesterols and lipoprotein (a) were measured by enzyme conventional methods. The TC serum varied not significantly in both subjects' groups as the triglycerides and HDL-C did. In addition, 58.46% of palm oil consumers had hypoLDLemia. The serum concentration of lipoprotein (a) was not significantly elevated (p> 0.05) with consumers compared to controls: 33.85% versus 29.09%, p = 0.55. The percentage of subjects with normal serum concentrations is higher in all the studied parameters, with both that is the consumers and the controls, except LDL cholesterol, of which the percentage of subjects with a lower value is the highest (58.46% for consumers and 52.73% for controls). This study has shown that the consumption of palm oil did not alter the lipid and lipoprotein profile of the consumer, on the contrary, this consumption revealed a decrease in cholesterol levels with these subjects.

show abstract

“…Freshly removed testes and epididymis were freed from adherent tissues weighed to the nearest milligram and biochemical studies such as estimations of DNA and RNA as per the method of Schnieider [17], protein by Lowry et al, [18], glycogen by Carrol et al, [19], cholesterol by Abell et al [20], sialic acid by Yao et al [21], activity of enzymes such as 3βHSD by Shivanandappa and Venkatesh [22], SDH by Nachlas et al, [23], LDH by King [24], Na + -K + ATPase, Ca ++ ATPase and Mg ++ ATPase were assayed according to the method described by Jinna et al, [25], ACP and AKP by Bergmeyer and Bernt [26], in testis and epididymis were carried out.…”

Section: Biochemical Estimationsmentioning

confidence: 99%

Methomyl induced gonadal dysfunction, biochemical contents and enzyme activities in male albino mice

Bb¹,

Si²

2010

Int J of Biot Appl

View full text Add to dashboard Cite

Abstract-Methomyl (S-methyl-1-N [(methyl carbamoyl) oxy] thio acetimidate, is a carbamate broad spectrum insecticide. The Swiss albino mice were administered orally 1, 2, 3, and 4 mg/kg body wt methomyl for 30 days and effective dose 4 mg/kg for 5, 10, and 20 days to know the dose and durational effect on testes and biochemical contents. The mice were sacrificed on day 31 st or 24 hours after the terminal exposure. Testes, epididymis, vasa deferentia, seminal vesicles, prostate, coagulatory and Cowper's glands weight decreased significantly with 2, 3 and 4 mg/kg/day and in 4mg/kg /day for 10 and 20 days of methomyl treatment.Histologic studies of the testes revealed anti-spermatogenic and anti-steroidogenic effects of methomyl reflected by significant decrease in the number and diameter of spermatogonia, primary spematocytes, secondary spermatocytes, spermatids and Leydig cells in the mice treated with 2, 3 and 4 mg/kg/day and in 4 mg/kg /day for 10 and 20 days of methomyl treatment, except with 2 mg/kg/day methomyl treatment number of primary spematocytes, Leydig cells, diameter of spermatogonia, and in 4 mg/kg/day methomyl for 10 days treatment the number of spermatogonia, leydig cells and diameter of primary spermatocytes not changed significantly when compared with controls. Biochemical studies of testis and epididymis showed methomyl at doses of 2, 3 and 4 mg/kg/day and in 4 mg/kg /day for 10 and 20 days of methomyl treatment caused significant decrease in the levels of DNA, RNA, protein, glycogen and sialic acid whereas cholesterol increased significantly. However, treatment with 1, 2 mg / kg/ day and 4 mg / kg /day for 5 days methomyl caused no significant change in epididymal biochemical contents when compared with control. Study on enzyme activities in testis and epididymis revealed that the treatment with 3 and 4 mg/kg/day and 4 mg/kg /day for 20 days of methomyl caused significant decrease in the activities of succinic dehydrogenase (SDH), ions ATPase (Na + -K + ATPase, Ca ++ ATPase, Mg ++ ATPase) and acid phosphatase (ACP) were decreased significantly, except with 3 mg/kg/day methomyl treatment where Ca ++ ATPase activity in testes and epididymis not changed significantly. However, lactate dehydrogenase (LDH) and alkaline phosphatase (AKP) activities were increased significantly. The testis 3β hydroxysteroid dehydrogenase (3βHSD) decreased significantly with 3 and 4 mg/kg /day and in 4 mg/kg /day for 10 and 20 days of methomyl treatment when compared with controls. However, there was no significant change in testes and accessory reproductive organs, number and diameter of spermatogonia and Leydig cells and biochemical contents of testis and epididymis with 1 mg/kg/day and in 4 mg/kg /day for 5 days of methomyl treatment. These observed effects of methomyl on testes and accessory reproductive organs weight, testis histology, biochemical contents and enzyme activities in testis and epididymis may be due to toxic effect or hormonal imbalance in any of the stage in the hypothalamo-hypophysial-testicular ...

show abstract

Cholesterol in Serum

Cited by 60 publications

References 4 publications

Comparison of 9 Methods for the Determination of Cholesterol

Comparison of 9 Methods for the Determination of Cholesterol

Effects of Palm Oil Consumption on Lipid Profile among Rural Ivorian Youth

Methomyl induced gonadal dysfunction, biochemical contents and enzyme activities in male albino mice

Contact Info

Product

Resources

About