“…The neuronal high-affinity choline transporter (CHT; SLC5A7 ) is the rate-limiting step for the synthesis of ACh (Ennis & Blakely, 2016; Ferguson et al, 2004; Guyenet, Lefresne, Rossier, Beaujuan, & Glowinski, 1973; Haga, 2014; Parikh, St. Peters, Blakely, & Sarter, 2013; Sarter & Parikh, 2005; Simon, Atweh, & Kuhar, 1976). As such, the CHT is a major determinant of the capacity of cholinergic neurons to sustain elevated levels of cholinergic neuromodulation (Paolone, Angelakos, et al, 2013; Paolone, Mallory, et al, 2013).…”