Cholinergic Enhancing and Antioxidant Effect of Vigna subterranea (L.) Verdc. (Fabaceae) Landrace Aqueous Extract on Scopolamine-Induced Amnesia in Male Swiss Mice

Abstract

The present study was conducted to investigate protective effects of the aqueous extract of V. subterranea seeds landrace on amnesia induced by scopolamine in mice. V. subterranea aqueous extract (100, 200 and 400 mg/kg BW) was administered by gavage for nine consecutive days and memory impairment was induced by repeated intraperitoneal injection of scopolamine (1.5 mg/kg). The Y-maze (YM), Morris water maze (MWM), novel object recognition paradigm (NOR) and the T maze (TM) were used to assess learning, memory and retention. Superoxide dismutase (SOD), Catalase (CAT), Malondialdehyde (MDA) levels and Acetylcholine esterase activity was also evaluated in the mice hippocampi homogenates. V. subterranea aqueous extract (400 mg/kg) significantly increased the percentage of spontaneous alternation in the YM task and decreased escape latency in the MWM. Moreover, this dose brought about a significantly improvement in the time spent in the preferred TM arm and discrimination index in the NOR tasks despite repeated scopolamine injection. Additionally, low acetylcholine esterase levels, reduced lipid peroxidation (malondialdehyde) but increased antioxidant enzymes (catalase and superoxide dismutase) activity was observed in hippocampi homogenate of mice pre-treated with the extract. A protective action against hippocampal cell damage was also evident. This finding suggests that the aqueous extract of V. subterranea seed landrace may improve learning and memory.

Consumption of underutilised grain legumes and the prevention of type II diabetes and cardiometabolic diseases: Evidence from field investigation and physicochemical analyses

“…This survey highlights the therapeutic potentials of Bambara groundnut seeds in the prevention of neurodegenerative diseases, diabetes, cardiometabolic diseases, and many other ailments. The primary collected data are in agreement with the in vivo work carried out on the study of the neuroprotective effects of BB' extracts [25]. According to Abaissou et al [25], aqueous extracts have a favourable effect on intelligence and memorisation.…”

“…The primary collected data are in agreement with the in vivo work carried out on the study of the neuroprotective effects of BB' extracts [25]. According to Abaissou et al [25], aqueous extracts have a favourable effect on intelligence and memorisation. Likewise, the potential antidiabetic and antihypertensive properties have been con rmed by Mune et al [26]; Arise and Malomo [27].…”

Cashew (Anacardium occidentale) Extract: Possible Effects on Hypothalamic–Pituitary–Adrenal (HPA) Axis in Modulating Chronic Stress

“…It was divided into four quadrants: north, south, east, and west. The testing procedure was conducted over 8 days, following the method employed by Ngatanko et al [79].…”