Chondrogenic differentiation of human bone marrow mesenchymal stem cells on polyhydroxyalkanoate (PHA) scaffolds coated with PHA granule binding protein PhaP fused with RGD peptide

You, Ming; Peng, Gongfeng; Li, Jian; Ma, Ping; Wang, Zhihui; Wang, Shu; Peng, Siwu; Chen, Guoqiang

doi:10.1016/j.biomaterials.2010.12.009

Cited by 115 publications

(104 citation statements)

References 48 publications

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“…151 Several studies demonstrated that peptide Arg-Gly-Asp (RGD) attachment increased MSC survival, proliferation, and differentiation. [156][157][158][159][160] Zhang et al demonstrated that RGD attached poly (caprolactone) (PCL) increased MSC proliferation and this was mediated by the activation of the PI3K/Akt pathway. 161 RGD activated an integrin that in turn increased Akt activity.…”

Section: Modification Of Biomaterials Surfacementioning

confidence: 99%

The Key Regulatory Roles of the PI3K/Akt Signaling Pathway in the Functionalities of Mesenchymal Stem Cells and Applications in Tissue Regeneration

Chen

Crawford

Chen

et al. 2013

Tissue Engineering Part B: Reviews

206

161

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Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various cell types and have been widely used in tissue engineering application. In tissue engineering, a scaffold, MSCs and growth factors are used as essential components and their interactions have been regarded to be important for regeneration of tissues. A critical problem for MSCs in tissue engineering is their low survival ability and functionality. Most MSCs are going to be apoptotic after transplantation. Therefore, increasing MSC survival ability and functionalities is the key for potential applications of MSCs. Several approaches have been studied to increase MSC tissue forming capacity including application of growth factors, overexpression of stem cell regulatory genes, and improvement of biomaterials for scaffolds. The effects of these approaches on MSCs have been associated with activation of the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway. The pathway plays central regulatory roles in MSC survival, proliferation, migration, angiogenesis, cytokine production, and differentiation. In this review, we summarize and discuss the literatures related to the roles of the PI3K/Akt pathway in the functionalities of MSCs and the involvement of the pathway in biomaterials-increased MSC functionalities. Biomaterials have been modified in their properties and surface structure and loaded with growth factors to increase MSC functionalities. Several studies demonstrated that the biomaterials-increased MSC functionalities are mediated by the activation of the PI3K/Akt pathway. MSCs are going to be apoptotic after transplantation. Therefore, increasing MSC survival ability and functionalities is the key for potential applications of MSCs. Several approaches have been studied to increase MSC tissue forming capacity including application of growth factors, overexpression of stem cell regulatory genes, and improvement of biomaterials for scaffolds. The effects of these approaches on MSCs have been associated with activation of the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway. The pathway plays central regulatory roles in MSC survival, proliferation, migration, angiogenesis, cytokine production, and differentiation. In this review, we summarize and discuss the literatures related to the roles of the PI3K/Akt pathway in the functionalities of MSCs and the involvement of the pathway in biomaterials-increased MSC functionalities. Biomaterials have been modified in their properties and surface structure and loaded with growth factors to increase MSC functionalities. Several studies demonstrated that the biomaterials-increased MSC functionalities are mediated by the activation of the PI3K/Akt pathway.

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Section: Modification Of Biomaterials Surfacementioning

confidence: 99%

The Key Regulatory Roles of the PI3K/Akt Signaling Pathway in the Functionalities of Mesenchymal Stem Cells and Applications in Tissue Regeneration

Chen

Crawford

Chen

et al. 2013

Tissue Engineering Part B: Reviews

206

161

View full text Add to dashboard Cite

show abstract

“…One of the advantages of 3D systems is the substantial surface area to volume ratio can maximize cell-material contact when compared to monolayer culture systems. An ideal scaffold for cartilage tissue engineering should be biocompatible, non-cytotoxic and have favorable structural features for cell attachment and proliferation (Lefebvre et al, 1997 andMingliang et al, 2011). This study showed that cells attached, proliferated and secreted extracellular matrix in the two 3D porous scaffolds used.…”

Section: Discussionmentioning

confidence: 90%

“…For instance, nowadays it is beyond dispute that scaffolds are sources of instructive signals for cell differentiation, migration, proliferation and orientation, and of paramount role in phenotype maintenance. Therefore, many studies have searched for great biomaterials that can be used as a surrogate for extracellular matrix (ECM) tissue (Ikada et al, 2006;Chiang et al, 2009;Tabata et al, 2009 andMingliang et al, 2011). One of the main goals of Tissue engineering is to create a scaffold that can mimic ECM due to better cells, and micro-environment interactions.…”

Section: Tissue Engineeringmentioning

confidence: 99%

Cartilage Tissue Engineering Using Mesenchymal Stem Cells and 3D Chitosan Scaffolds – In vitro and in vivo Assays

Breyner¹,

Zonari²,

Carvalho³

et al. 2011

Biomaterials Science and Engineering

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“…It has been speculated that hMSC can thrive especially well in the undifferentiated state in presence of RGD [10]. Recently, it has been investigated whether hMSCs can be pushed towards the discogenic pathway by the addition of RGD motive [13,14]. The recent study found a weaker effect of transforming and growth factor β 3 (TGFβ 3 ) [10] if RGD motive was added to an alginate culture system.…”

Section: Introductionmentioning

confidence: 99%

Mechanical Loading Promoted Discogenic Differentiation of Human Mesenchymal Stem Cells Incorporated in 3D-PEG Scaffolds with rhGDF5 and RGD

Guggisberg¹,

Benneker

Keel³

et al. 2015

Int J Stem Cell Res Ther

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Chondrogenic differentiation of human bone marrow mesenchymal stem cells on polyhydroxyalkanoate (PHA) scaffolds coated with PHA granule binding protein PhaP fused with RGD peptide

Cited by 115 publications

References 48 publications

The Key Regulatory Roles of the PI3K/Akt Signaling Pathway in the Functionalities of Mesenchymal Stem Cells and Applications in Tissue Regeneration

The Key Regulatory Roles of the PI3K/Akt Signaling Pathway in the Functionalities of Mesenchymal Stem Cells and Applications in Tissue Regeneration

Cartilage Tissue Engineering Using Mesenchymal Stem Cells and 3D Chitosan Scaffolds – In vitro and in vivo Assays

Mechanical Loading Promoted Discogenic Differentiation of Human Mesenchymal Stem Cells Incorporated in 3D-PEG Scaffolds with rhGDF5 and RGD

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