Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo

Park, Jin-Sung; Lee, Hyun Jae; Lee, Dong Hoon; Jo, Ho Seung; Jeong, Junhui; Kim, Dong Hee; Nam, Dae Cheol; Lee, Choong Jae; Hwang, Sun‐Chul

doi:10.4062/biomolther.2015.045

Cited by 30 publications

(25 citation statements)

References 32 publications

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“…Chlorogenic acid [17] and Caffeic acid [43] have inhibitory effects on the inflammatory proliferation of synoviocytes. Wogonin can down-regulate the production of MMP-3, acting directly on articular chondrocytes [44]. Wogonoside inhibits LPS-induced angiogenesis both in vitro and in vivo [45].…”

Section: Discussionmentioning

confidence: 99%

Herbal formula Xian-Fang-Huo-Ming-Yin regulates differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis mice

Li¹,

Wei²,

Li³

et al. 2017

BMC Complement Altern Med

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BackgroundXian-Fang-Huo-Ming-Yin (XFHM), a traditional herbal formula, has been used to treat sores and carbuncles for hundreds of years in Asia. Nowadays, its clinical effects in treatment of rheumatoid arthritis (RA) have been validated. In this study, we want to study its possible molecular mechanisms of regulating the differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis (CIA) mice for RA treatment.MethodsA high performance liquid chromatography-electrospray ionization/mass spectrometer (HPLC-ESI/MSn) system was used to analyze the constituents of XFHM granules. An arthritics mouse model was induced by collagen and leflunomide (LEF) was used as a positive control medicine. Pathological changes at the metatarsophalangeal joint were studied through Safranin O and immunohistochemical staining. The differentiation of T, B and NK cells was examined by flow cytometry and pro-inflammatory cytokines were assayed using an Inflammation Antibody Array assay. The expression of key molecules of the nuclear factor κB (NF-κB) and Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathways in spleen were studied by western-blot analysis.ResultsIn our study. 21 different dominant chemical constituents were identified in XFHM. Treatment with XFHM suppressed the pathological changes in arthrosis of CIA. Additionally, XFHM down-regulated the proliferation and differentiation of CD3+ T cells and CD3−CD19+ B cells significantly. However, XFHM had no significant effect on CD3−NK1.1+ NK cells. Further study showed that the production of pro-inflammatory cytokines had been suppressed by inhibiting the activation of NF-κB and JAK/STAT signaling.ConclusionsXFHM can regulate and maintain the immunologic balance of lymphocytic immunity and inhibit the production of pro-inflammatory cytokines, thus suppressing the pathological changes of RA. Therefore, XFHM may be used as an application of traditional medicine against RA in modern complementary and alternative therapeutics.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1526-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Herbal formula Xian-Fang-Huo-Ming-Yin regulates differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis mice

Li¹,

Wei²,

Li³

et al. 2017

BMC Complement Altern Med

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“…Imbalance between MMPs and TIMPs is a salient feature of OA progression, leading to disruption of the balance between ECM biosynthesis and degradation [28]. Therefore, exploration of the mechanisms through which the proteolytic activity, production and expression of MMPs are inhibited by plant-derived natural products may lead to new therapeutic strategies [34]. To determine whether OA-F2 may affect the destruction of articular cartilage, we examined the expression of the OA biomarkers MMP-3, MMP-9 and TIMP-1 in the synovium of collagenase-induced OA rats.…”

Section: Discussionmentioning

confidence: 99%

New herbal composition (OA-F2) protects cartilage degeneration in a rat model of collagenase induced osteoarthritis

Nirmal

Jagtap

Narkhede

et al. 2017

BMC Complement Altern Med

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BackgroundPrevalence of osteoarthritis (OA) is on rise on the global scale. At present there are no satisfactory pharmacological agents for treating OA. Our previous study showed that Sida cordifolia L. and Zingiber officinale Rosc. had protective effect on cartilage. Here, we describe the effect of OA-F2, a herbal formulation prepared using combination of these two plants in alleviating OA associated symptoms in a rat model of collagenase-induced OA.MethodsOA was induced by intra-articular injection of collagenase type II in wistar rats. Diclofenac (10 mg/kg) was used as a reference control. Rats (n = 6) were divided into 6 groups: Healthy control (HC), osteoarthritic control (OAC), diclofenac (DICLO), OA-F2L (135 mg/kg), OA-F2M (270 mg/kg) and OA-F2H (540 mg/kg). The effects of the 20 days treatment were monitored by parameters like knee diameter, paw volume, paw retraction; serum C-reactive protein (CRP), alkaline phosphatase (ALP) and glycosaminoglycan (GAG). Radiography and histopathology of knee joint were also studied. Additionally, gene expression was studied from isolated synovium tissue proving anti-osteoarthritic potential of OA-F2.ResultsOral administration of OA-F2 has significantly prevented knee swelling compared to OAC; OA-F2 and DICLO, significantly reduced paw volume compared to OAC. Paw latency was remarkably increased by OA-F2 compared to OAC. OA-F2L (−0.670, p < 0.001), M (−0.110, p < 0.05) and H (0.073) has markedly reduced levels of CRP compared to DICLO. OA-F2L (p < 0.05), M (p < 0.001) and H (p < 0.05) significantly reduced ALP levels, compared to DICLO. GAG release in the serum was also significantly lowered in OA-F2 treated group compared to DICLO. Radiological and histopathological observations showed cartilage protection by OA-F2. OA-F2 has upregulated SOD and GPx. Upregulated CAT expression was observed in OA-F2M and H. Considerable down-regulation of expression of MMP-3 and MMP-9 was observed in all the groups. Up-regulation of TIMP-1 was observed in rats treated with OA-F2L, H and DICLO.ConclusionOA-F2 has shown therapeutic effects in rat model of collagenase induced OA by demonstrating cartilage protection through controlling MMPs and improving anti-oxidant levels in arthritic synovium and is a potent candidate for further drug development and treatment for OA.

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“…36 Wogonin has also been shown to suppress key mediators of oxidative stress, inhibit inflammation, and matrix degradation via suppression of major proteases, including matrix metalloproteinase (MMP)-3, MMP-9, and MMP-13, and promote the expression of chondrogenic markers such as COL2A1 and aggrecan (AGC) in OA chondrocytes and cartilage explants. [37][38][39] Above all, wogonin can bind to DNA via incorporation as a DNA intercalator and minor groove binder. 40 In addition to maintaining the morphology of chondrocytes, enhancing chondrocyte pro- Table 1.…”

Section: Introductionmentioning

confidence: 99%

Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis

et al. 2020

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Osteoarthritis, a disorder characterized by articular cartilage deterioration, varying degrees of inflammation, and chondrocyte apoptosis, is the most common chronic joint disease. To slow or reverse its progression, inflammation should be inhibited, and chondrocyte proliferation should be promoted. Tetrahedral framework nucleic acids can be internalized by chondrocytes (even inflammatory chondrocytes) and can enhance their proliferation and migration. Wogonin, a naturally occurring flavonoid, suppresses oxidative stress and inhibits inflammation. In this study, tetrahedral framework nucleic acids were successfully selfassembled and used to load wogonin. We confirmed the effective formation of tetrahedral framework nucleic acid/wogonin complexes by dynamic light scattering, zeta potential analysis, transmission electron microscopy, and fluorescence spectrophotometry. Tetrahedral framework nucleic acids, wogonin, and especially tetrahedral framework nucleic acid/wogonin complexes effectively alleviated inflammation in vitro and in vivo and prevented cartilage destruction. In addition, these materials remarkably downregulated the expression of inflammatory mediators and matrix metalloproteinases, upregulated chondrogenic markers, and promoted tissue inhibitor of metalloproteinase 1 and B-cell lymphoma 2 expression. In vivo, after treatment with tetrahedral framework nucleic acid/wogonin complexes, the bone mineral density in regenerated tissues was much higher than that found in the untreated groups. Histologically, the complexes enhanced new tissue regeneration, significantly suppressed chondrocyte apoptosis, and promoted chondrogenic marker expression. They also inhibited cell apoptosis, increased chondrogenic marker expression, and suppressed the expression of inflammatory mediators in osteoarthritis. Therefore, we believe that tetrahedral framework nucleic acid/wogonin complexes can be used as an injectable form of therapy for osteoarthritis.Bone Research (2020) 8:6; https://doi.

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Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo

Cited by 30 publications

References 32 publications

Herbal formula Xian-Fang-Huo-Ming-Yin regulates differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis mice

Herbal formula Xian-Fang-Huo-Ming-Yin regulates differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis mice

New herbal composition (OA-F2) protects cartilage degeneration in a rat model of collagenase induced osteoarthritis

Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis

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