Chorioallantoic Membrane Angiogenesis Model for Tissue Engineering: A New Twist on a Classic Model

Borges, J.; Tegtmeier, F. T.; Padron, Nestor Torio; Mueller, Matthias; Lang, Eva; Stark, G. B.

doi:10.1089/107632703322066624

Cited by 79 publications

(68 citation statements)

References 21 publications

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“…Prior studies have reported vascularizaton of tissue engineered constructs using both synthetic and naturally-derived constructs containing growth factors and/or cells (for examples see (Borges et al, 2003a;Borges et al, 2003b;Elcin and Elcin, 2006;Ganta et al, 2003;Kaigler et al, 2006;Murphy et al, 2004;Tanihara et al, 2001;Yao et al, 2004;Zisch et al, 2003)). However, the primary focus of these studies did not address the manner of directed vascular invasion into the matrix, but only upon the end extent of vacularization.…”

Section: Discussionmentioning

confidence: 99%

“…The chorioallantoic membrane (CAM) assay, used for more than half a century, has become a mainstay in the study of blood vessel development (Auerbach et al, 2003;Cruz et al, 2000;Cruz et al, 1997;DeFouw and DeFouw, 2000a;DeFouw and DeFouw, 2000b;Djonov et al, 2000a;Djonov et al, 2000b;McDonnell et al, 2005;Rizzo and DeFouw, 1996;Rizzo et al, 1995;Rizzo et al, 1993). The CAM assay has also proved useful for biological devices and in tissue engineering applications, particularly those concerning biocompatibility studies and angiogenic responses to tissue-engineered constructs (Borges et al, 2003a;Borges et al, 2003b;Nguyen et al, 1994;Ribatti et al, 2001;Rickert et al, 2003;Valdes et al, 2003;Valdes et al, 2002;Wong et al, 2003). In this work we extend the CAM assay in the development of a histological technique to specifically examine direct vascular invasion into fibrin-based constructs.…”

Section: Introductionmentioning

confidence: 99%

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Improved growth factor directed vascularization into fibrin constructs through inclusion of additional extracellular molecules

Smith

Melhem

Magge

et al. 2007

Microvascular Research

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Using the chick chorioallantoic membrane assay (CAM) and a novel histological technique we investigated the ability of blood vessels to directly invade fibrin-based scaffolds. In our initial experiments utilizing vascular endothelial growth factor (VEGF 165 ) we found no direct invasion. Instead, the fibrin was completely degraded and replaced with highly vascularized new tissue. Addition of fibroblast growth factor-2 (FGF-2), bone morphogenic protein-2 (BMP-2), or plateletderived growth factor-BB (PDGF-BB) to the fibrin construct also did not result in construct vascularization. Because natural and regenerating tissues exhibit complex extracellular matrices (ECMs), we hypothesized that a more complex scaffold may improve blood vessel invasion. Addition of fibronectin, hyaluronic acid, and collagen type I within 20 mg/mL fibrin constructs resulted in no significant improvement. However, the same additive concentrations within 10 mg/ mL fibrin constructs resulted in dramatic improvements, specifically with hyaluronic acid. Overall, we believe these results indicate the importance of structural and functional cues of not only in the initial scaffold but also as the construct is degraded and remodeled. Furthermore, the CAM assay may represent a useful model for understanding ECM interactions as well as for screening and designing tissue engineered scaffolds.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improved growth factor directed vascularization into fibrin constructs through inclusion of additional extracellular molecules

Smith

Melhem

Magge

et al. 2007

Microvascular Research

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“…The CAM system has proved to be an effective method of confirming the angiogenic properties of novel scaffold materials, with or without cells or angiogenic factors, for potential application in critical size bone grafts as well as for assessing the susceptibility of these materials to vessel invasion (Baiguera et al, 2012;Borges et al, 2003;Kanczler et al, 2007). Exemplar studies include: the testing of 45S5 Bioglass-derived glass-ceramic scaffolds for biocompatibility and bone induction (Vargas et al, 2009); spheroidal osteoblast and endothelial cell cocultures for induction of perfused blood vessel networks into scaffolds (Steffens et al, 2009); porous polycaprolactone (PCL) scaffolds incorporating VEGF for stimulation of angiogenesis (Singh et al, 2012); and DegraPol ® foam scaffolds seeded with human osteoblasts and endothelial cells for angiogenic induction (Buschmann et al, 2011).…”

Section: El Smith Et Al Chick Limb Organ Culture In the 21 St Centurymentioning

confidence: 99%

“…A wide variety of bioreactor types exist that include rotating oxygen-diffusing vessels, filled with culture media, and perfusion systems incorporating controlled flow back and forth within the construct. These systems have been shown to increase cell proliferation and enhance osteoblast differentiation/osteogenesis (Botchwey et al, 2001;Granet et al, 1998;Kavlock and Goldstein, 2008;Milan et al, 2009;Pound et al, 2006;Sikavitsas et al, 2003). In addition, perfusion systems can be combined with application of mechanical forces using compression bioreactors to maintain viable bone explants (El Haj et al, 1990) and significantly increase osteogenesis in cell-seeded scaffolds (Bolgen et al, 2008).…”

Section: El Smith Et Almentioning

confidence: 99%

A new take on an old story: chick limb organ culture for skeletal niche development and regenerative medicine evaluation

Smith

Kanczler²,

Oreffo³

2013

eCM

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Scientific research and progress, particularly in the drug discovery and regenerative medicine fields, is typically dependent on suitable animal models to develop new and improved clinical therapies for injuries and diseases. In vivo model systems are frequently utilised, but these models are expensive, highly complex and pose a number of ethical considerations leading to the development and use of a number of alternative ex vivo model systems. The ex vivo embryonic chick long bone and limb bud models have been utilised in the scientific research field as a model to understand skeletal development for over eighty years. The rapid development of avian skeletal tissues, coupled with the ease of experimental manipulation, availability of genome sequence and the presence of multiple cell and tissue types has seen such model systems gain significant research interest in the last few years in the tissue engineering field. The models have been explored both as systems for understanding the developmental bone niche and as potential testing tools for tissue engineering strategies for bone repair and regeneration. This review details the evolution of the chick limb organ culture system and presents recent innovative developments and emerging techniques and technologies applied to these models that are aiding our understanding of skeletal developmental and regenerative medicine research and application.

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“…Several studies have shown that mammalian cells and tissues transplanted to avian embryos can respond to local cues and develop into tissues appropriate to their location in the host (Fontaine-Perus et al 1997;Goldstein et al 2002;White and Anderson 1999). This has been used for different applications of the CAM model such as angiogenesis and antiangiogenesis (Ribatti et al 2000(Ribatti et al , 2001Richardson and Singh 2003), wound healing (Ribatti et al 1996), tissue engineering (Borges et al 2003), biomaterials and implants Valdes et al 2002;Zwadlo-Klarwasser et al 2001), testing therapeutics on human leukemia (Taizi et al 2006), short-term transplantation of cryopreserved human ovarian tissue (Martinez-Madrid et al 2009) and biosensors (Valdes et al 2003). Chick embryos have also been used as a host for the study of the migration of mouse embryonic stem cells with and without genetic manipulation (Beauvais-Jouneau et al 1999).…”

Section: Introductionmentioning

confidence: 99%

New system for real time study of in vivo migration and differentiation of stem cells

Haque

Fuhr

2010

Microsyst Technol

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Cell therapy is a promising and emerging field for the treatment of human diseases. However, to understand and optimize cell therapy, the inability to track the cell transplants in vivo remains a major problem. Most cell transplantation techniques involve the use of histological analysis to evaluate cell transfection, proliferation, and migration after sacrifice. In this literature, for the first time an in vivo model has been developed to study stem cells. Explantation culture of chicken embryo into surrogate shells has been modified for high resolution and long term imaging. A special long distance fluorescence microscope and micromanipulation systems has been developed for in vivo application specially aiming for the injection and tracking of fluorescence labeled cells into chicken embryo and track them. By using the developed system, it was possible to image the whole period of embryonic development of chicken embryo.

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Chorioallantoic Membrane Angiogenesis Model for Tissue Engineering: A New Twist on a Classic Model

Cited by 79 publications

References 21 publications

Improved growth factor directed vascularization into fibrin constructs through inclusion of additional extracellular molecules

Improved growth factor directed vascularization into fibrin constructs through inclusion of additional extracellular molecules

A new take on an old story: chick limb organ culture for skeletal niche development and regenerative medicine evaluation

New system for real time study of in vivo migration and differentiation of stem cells

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