Chorion-derived extracellular matrix hydrogel and fibronectin surface coatings show similar beneficial effects on endothelialization of expanded polytetrafluorethylene vascular grafts

Rohringer, Sabrina; Schneider, Karl H.; Eder, Gabriela; Hager, Pia; Enayati, Marjan; Kapeller, Barbara; Kiss, Herbert; Windberger, Ursula; Podesser, Bruno K.; Bergmeister, Helga

doi:10.1016/j.mtbio.2022.100262

Cited by 12 publications

(6 citation statements)

References 93 publications

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“…Few examples of such studies are discussed here in comparison with the ECM-coated nanotextile woven patch. A human placental chorionic ECM hydrogel-coated ePTFE scaffold showed good EC adhesion and proliferation . Likewise, coating of cell-secreted ECM on the ePTFE scaffold with heparin immobilization enabled endothelialization, with selective inhibition of SMCs .…”

Section: Resultsmentioning

confidence: 97%

Section: Assessment Of Collagen Depositionmentioning

confidence: 99%

“…A human placental chorionic ECM hydrogel-coated ePTFE scaffold showed good EC adhesion and proliferation. 28 Likewise, coating of cell-secreted ECM on the ePTFE scaffold with heparin immobilization enabled endothelialization, with selective inhibition of SMCs. 2 Similarly, endothelial ECM-based biomimetic coating provided on hyaluronic acid micropatterned surface and further decellularized in a layer-by-layer pattern displayed good proendothelialization function and tissue compatibility.…”

Section: Assessment Of Collagen Depositionmentioning

confidence: 99%

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Human Adipose-Derived Mesenchymal Stem Cell-Secreted Extracellular Matrix Coating on a Woven Nanotextile Vascular Patch for Improved Endothelial Cell Response

Gopal

Nandakumar

Reshmi

et al. 2023

ACS Appl. Bio Mater.

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Biomedical implants possessing the structural and functional characteristics of extracellular matrix (ECM) are pivotal for vascular applications. This study investigated the potential of recreating a natural ECM-like structural and functional environment on the surface of biodegradable polymeric nanotextiles for vascular implants. Human adipose-derived mesenchymal stem cells (MSCs) were grown on a suitably engineered polycaprolactone (PCL) nanofibrous textile and were allowed to modify its surface through the deposition of MSC-specific ECM. This surface-modified nanotextile showed mechanical characteristics and functionality appropriate for vascular patch material. The uniformity of ECM coating significantly improved the viability, proliferation, and migration of human endothelial cells compared to bare and xenogeneic collagen-coated PCL nanotextile patches. Thus, a polymeric nanotextile, which is surface modified using MSC-driven ECM, provided a rapid and improved endothelialization, thereby suggesting its potential for vascular patch applications.

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Section: Resultsmentioning

confidence: 97%

Section: Assessment Of Collagen Depositionmentioning

confidence: 99%

Section: Assessment Of Collagen Depositionmentioning

confidence: 99%

See 1 more Smart Citation

Human Adipose-Derived Mesenchymal Stem Cell-Secreted Extracellular Matrix Coating on a Woven Nanotextile Vascular Patch for Improved Endothelial Cell Response

Gopal

Nandakumar

Reshmi

et al. 2023

ACS Appl. Bio Mater.

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“…Chemical coupling of VEGF to graft material has also been used in efforts to enhance graft re-endothelialization [ 41 ]. Importantly, we recently found that a luminal coating of placental ECM hydrogel on ePTFE vascular grafts more strongly promotes re-endothelialization than that achieved with fibronectin coats [ 42 ], and in future work, we aim to investigate whether we can apply this method to submillimeter grafts with similar success.…”

Section: Discussionmentioning

confidence: 99%

Acellular Human Placenta Small-Diameter Vessels as a Favorable Source of Super-Microsurgical Vascular Replacements: A Proof of Concept

et al. 2023

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In this study, we aimed to evaluate the human placenta as a source of blood vessels that can be harvested for vascular graft fabrication in the submillimeter range. Our approach included graft modification to prevent thrombotic events. Submillimeter arterial grafts harvested from the human placenta were decellularized and chemically crosslinked to heparin. Graft performance was evaluated using a microsurgical arteriovenous loop (AVL) model in Lewis rats. Specimens were evaluated through hematoxylin-eosin and CD31 staining of histological sections to analyze host cell immigration and vascular remodeling. Graft patency was determined 3 weeks after implantation using a vascular patency test, histology, and micro-computed tomography. A total of 14 human placenta submillimeter vessel grafts were successfully decellularized and implanted into AVLs in rats. An appropriate inner diameter to graft length ratio of 0.81 ± 0.16 mm to 7.72 ± 3.20 mm was achieved in all animals. Grafts were left in situ for a mean of 24 ± 4 days. Decellularized human placental grafts had an overall patency rate of 71% and elicited no apparent immunological responses. Histological staining revealed host cell immigration into the graft and re-endothelialization of the vessel luminal surface. This study demonstrates that decellularized vascular grafts from the human placenta have the potential to serve as super-microsurgical vascular replacements.

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“…Endothelial progenitor cells (EPCs) were isolated as previously described. [ 80 ] In brief, freshly donated blood from healthy male and female donors (45 mL) was collected by venipuncture into sodium citrate tubes (Vacuette, Greiner Bio‐One, Austria) after informed consent and approval by the local ethics committee of the Medical University of Vienna (EK nr. 2321/2020).…”

Section: Methodsmentioning

confidence: 99%

Biodegradable, Self‐Reinforcing Vascular Grafts for In Situ Tissue Engineering Approaches

Rohringer

Grasl

Ehrmann

et al. 2023

Adv Healthcare Materials

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Clinically available small‐diameter synthetic vascular grafts (SDVGs) have unsatisfactory patency rates due to impaired graft healing. Therefore, autologous implants are still the gold standard for small vessel replacement. Bioresorbable SDVGs may be an alternative, but many polymers have inadequate biomechanical properties that lead to graft failure. To overcome these limitations, a new biodegradable SDVG is developed to ensure safe use until adequate new tissue is formed. SDVGs are electrospun using a polymer blend composed of thermoplastic polyurethane (TPU) and a new self‐reinforcing TP(U‐urea) (TPUU). Biocompatibility is tested in vitro by cell seeding and hemocompatibility tests. In vivo performance is evaluated in rats over a period for up to six months. Autologous rat aortic implants serve as a control group. Scanning electron microscopy, micro‐computed tomography (µCT), histology, and gene expression analyses are applied. TPU/TPUU grafts show significant improvement of biomechanical properties after water incubation and exhibit excellent cyto‐ and hemocompatibility. All grafts remain patent, and biomechanical properties are sufficient despite wall thinning. No inflammation, aneurysms, intimal hyperplasia, or thrombus formation are observed. Evaluation of graft healing shows similar gene expression profiles of TPU/TPUU and autologous conduits. These new biodegradable, self‐reinforcing SDVGs may be promising candidates for clinical use in the future.

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Chorion-derived extracellular matrix hydrogel and fibronectin surface coatings show similar beneficial effects on endothelialization of expanded polytetrafluorethylene vascular grafts

Cited by 12 publications

References 93 publications

Human Adipose-Derived Mesenchymal Stem Cell-Secreted Extracellular Matrix Coating on a Woven Nanotextile Vascular Patch for Improved Endothelial Cell Response

Human Adipose-Derived Mesenchymal Stem Cell-Secreted Extracellular Matrix Coating on a Woven Nanotextile Vascular Patch for Improved Endothelial Cell Response

Acellular Human Placenta Small-Diameter Vessels as a Favorable Source of Super-Microsurgical Vascular Replacements: A Proof of Concept

Biodegradable, Self‐Reinforcing Vascular Grafts for In Situ Tissue Engineering Approaches

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