Chorionic gonadotropin alleviates thioglycollate-induced peritonitis by affecting macrophage function

Abstract

Human chorionic gonadotrophin (hCG) is a hormone produced during pregnancy and present at the implantation site and in the maternal blood. Pregnancy has been proposed to represent a controlled state of inflammation at an early stage at the implantation site and later, systemically extended to the maternal circulation. Earlier, we reported that hCG can inhibit the development of diabetes in NOD mice and LPS-induced septic shock in a murine model. We hypothesize that hCG can contribute to the reduction of inflammation by modifying Mphi function. Here, the TG-induced peritonitis model for inflammation was used to investigate the effect of hCG on cytokine production and cell recruitment in vivo. hCG pretreatment in TG-induced peritonitis increased the number of peritoneal cells, especially PMN and monocytes, compared with mice injected with TG only. This increased cell number was partially explained by increased cell survival induced by hCG. Despite the cellular infiltrate, hCG pretreatment decreased i.p. TNF-alpha, IL-6, PTX3, CCL3, and CCL5 levels. By depleting peritoneal resident Mphi using clodronate liposomes prior to the application of hCG and the TG trigger, we established that Mphi are the main responsive cells to hCG, as the suppressed TNF-alpha and IL-6 production and increased PMN influx are abolished in their absence. Together, these data suggest that hCG contributes to the controlled inflammatory state of pregnancy by regulating Mphi proinflammatory function.

Evaluation of 3-(3-chloro-phenyl)-5-(4-pyridyl)-4,5-dihydroisoxazole as a Novel Anti-Inflammatory Drug Candidate

“…to induce peritonitis. Similar to previous data [17], 4 h after thioglycollate injection, we found an increase of inflammatory cells (Figure 8A), mainly consisting of neutrophils (81%), as shown in Figure 8B. In the control group without thioglycollate injection, only resident macrophages were found in the peritoneal cavity (Figure 8B, first column).…”

Dietary β-glucan Leads to Increased TNF-α Production in the Lung

“…We report that leukocytes from mice that consumed β-glucan expressed higher levels of TNF-α. This is consistent with the fact that interperitoneal injection with thioglycollate broth has been shown to induce an inflammatory response that includes not only recruitment of inflammatory cells but also increases in the level of inflammatory cytokines such as TNF-α (Wan et al, 2009). Fig.…”

Oral Administration of Nano-Emulsion Curcumin in Mice Suppresses Inflammatory-Induced NFκB Signaling and Macrophage Migration

“…Intraperitoneal injection of sterile thioglycollate medium in mice has been shown to induce an acute inflammatory response in the peritoneum. While the initial cells mediating the inflammation over the first 24 h consist primarily of neutrophils, macrophages predominate the peritoneal infiltrate after 3 days [25]. At later time points, T-cells and B-cells play important roles in the immune response, but are detected at lower levels [26].…”