Chromate tolerance and accumulation in Chlorella vulgaris L.: Role of antioxidant enzymes and biochemical changes in detoxification of metals

Rai, Umesh; Singh, Naveen; Upadhyay, Atul Kumar; Verma, S. R.

doi:10.1016/j.biortech.2013.03.043

Cited by 91 publications

(52 citation statements)

References 26 publications

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“…In the present study, Cd(II) stress could damage the biosynthesis of chlorophyll in C. vulgaris , which is in agreement with results of Küpper et al (2003), Rai et al (2013), and Çelekli et al (2016). The increase of Chlorophyll a / b suggesting that the Chl b is more sensitive than Chl b under 0.5 mg L −1 , the decrease suggesting that cadmium can cause some Chl a to be converted to Chl b by oxidation of the methyl group on the ring II (Chettri et al 1998).…”

Section: Discussionsupporting

confidence: 94%

“…Pigment reduction was reported for S. quadricauda exposed to Cu (Kováčik et al 2010) C. pyrenoidosa with perfluorooctanoic acid exposure (Xu et al 2013), S. obliquus exposed to Cu (Chen et al 2012) and carbamazepine (Zhang et al 2012), and C. vulgaris exposed to Cr (Rai et al 2013) and dichloromethane and dichloroethane (Wu et al 2014). In the present study, Cd(II) stress could damage the biosynthesis of chlorophyll in C. vulgaris , which is in agreement with results of Küpper et al (2003), Rai et al (2013), and Çelekli et al (2016).…”

Section: Discussionmentioning

confidence: 99%

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The effect of cadmium on the growth and antioxidant response for freshwater algae Chlorella vulgaris

et al. 2016

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The objective of the present work was to evaluate the effect of exogenously applied cadmium on the physiological response of green algae Chlorella vulgaris. The study investigated the long-term effect (18 days) of cadmium on the levels of algae biomass, assimilation pigment composition, soluble protein, oxidative status (production of hydrogen peroxide and superoxide anion), antioxidant enzymes (such as superoxide dismutase, peroxidase, catalase and glutathione reductase enzyme) in C. vulgaris. The results showed that growth, the amount of chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoids gradually decreased with increasing cadmium over 18 days exposure. Cadmium at concentration of 7 mg L−1 inhibited algal growth expressed as the number of cells. Our research found that C. vulgaris has a high tolerance to cadmium. Contents of chlorophylls (Chl a and Chl b) and carotenoids (Car) of C. vulgaris was significantly decline with rising concentration of cadmium (p < 0.05). The decrease of 54.04 and 93.37 % in Chl a, 60.65 and 74.32 % in Chl b, 50.00 and 71.88 % in total carotenoids was noticed following the treatment with 3 and 7 mg L−1 cadmium doses compared with control treatment, respectively. Cadmium treatments caused a significant change in the physiological competence (calculated as chlorophyll a/b) which increased with increasing Cd(II) doses up to 1 mg L−1 but decreased at 3 mg L−1. While accumulation of soluble protein was enhanced by presence of cadmium, the treatment with cadmium at 3 and 7 mg L−1 increased the concentration of soluble proteins by 88, 95.8 % in C. vulgaris, respectively. Moreover, low doses of cadmium stimulated enzymatic (superoxide dismutase, catalase and glutathione reductase) in C. vulgaris, The content of peroxidase increased with the increasing cadmium concentration, and had slightly decreased at the concentration of 7 mg L−1, but was still higher than control group, which showed that cadmium stress at high concentration mainly peroxidase works in C. vulgaris. And therefore, suppressed reactive oxygen species (hydrogen peroxide and superoxide) accumulated. The present study also showed that cadmium increased oxidative stress and induced antioxidant defense systems against reactive oxygen species. The observation in here analyzed C. vulgaris after exposure to cadmium indicate that hydrogen peroxide, superoxide and peroxidase in the alga with exposure to Cd(II) seemed to be parameters as biomarkers for metal-induced oxidative stress.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

The effect of cadmium on the growth and antioxidant response for freshwater algae Chlorella vulgaris

et al. 2016

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“…The amount of MDA varied significantly ( p < 0.05) from 4.87 to 5.99 μg/g ( p < 0.05), when Cd 2+ concentrations increased from 0 to 30 mg/L. Increasing MDA content of algae due to exposure of heavy metal has been previously studied by many researchers .…”

Section: Resultsmentioning

confidence: 95%

Morphological and Biochemical Responses of Spirogyra setiformis Exposed to Cadmium

Çelekli

Gültekin

Bozkurt

2015

CLEAN Soil Air Water

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“…Detoxification usually is a result of multiple mechanisms joint action. Several studies have indicated that Cd stress can hinder chlorophyll biosynthesis (Küpper et al, 2007; Redondo-Gómez et al, 2009; Rai et al, 2013; Çelekli et al, 2015; Li et al, 2015; Cheng et al, 2016). In the present study, GmORG3 reduced the damage of Cd stress in the transgenic tobacco plants and increased the chlorophyll content.…”

Section: Discussionmentioning

confidence: 99%

The Soybean Basic Helix-Loop-Helix Transcription Factor ORG3-Like Enhances Cadmium Tolerance via Increased Iron and Reduced Cadmium Uptake and Transport from Roots to Shoots

Liu

et al. 2017

Front. Plant Sci.

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Cadmium (Cd) is one of the most dangerous heavy metal pollutants in the environment and is toxic to animal and plant cells. On the other hand, iron (Fe) is an essential element for plant growth and development. The chlorosis of plant leaves under cadmium stress is similar to the typical symptom of iron deficiency. Recently, several Arabidopsis basic/helix-loop-helix (bHLH) transcription factors have been identified that are involved in the interactions between Cd and Fe. In the present study, over-expression the ORG3-like gene GmORG3, a bHLH transcription factor OBP3-responsive gene (ORG), enhanced Cd tolerance and stabilized Fe homeostasis. The domain analysis of GmORG3 showed that the protein contains a conserved 61-residue bHLH domain belonging to subfamily II. Moreover, subcellular localization experiments showed that GmORG3 is a nucleoprotein. GmORG3 was transcribed only in soybean roots and was significantly induced by external Cd stress in soybean plants. Heterologous expression of GmORG3 enhanced Cd tolerance in yeast. Furthermore, the overexpression of GmORG3 in soybean mosaic seedlings using a hairy root system showed that overexpressing plants increased the translocation ratio of Fe but reduced Cd translocation from the roots to shoots. In addition, the ectopic expression of GmORG3 in tobacco reduced phytotoxic effects induced by Cd stress and Fe deficiency, including the blockage of root elongation and decreased chlorophyll content. By integrating all these results, we found that GmORG3 plays an important role in response to Cd stress. The results provide new insights into the molecular mechanisms of Cd tolerance in soybean.

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Chromate tolerance and accumulation in Chlorella vulgaris L.: Role of antioxidant enzymes and biochemical changes in detoxification of metals

Cited by 91 publications

References 26 publications

The effect of cadmium on the growth and antioxidant response for freshwater algae Chlorella vulgaris

The effect of cadmium on the growth and antioxidant response for freshwater algae Chlorella vulgaris

Morphological and Biochemical Responses of Spirogyra setiformis Exposed to Cadmium

The Soybean Basic Helix-Loop-Helix Transcription Factor ORG3-Like Enhances Cadmium Tolerance via Increased Iron and Reduced Cadmium Uptake and Transport from Roots to Shoots

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