Chromatic Aberration Correction

Leary, Rowan K.; Brydson, Rik

doi:10.1016/b978-0-12-385861-0.00003-8

Cited by 12 publications

(2 citation statements)

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“…The spherical aberration was removed using correctors and aspherical lenses ( Fan et al, 2017 ). To remove chromatic aberration with a FEG and a monochromator, a chromatic aberration correctors composed of an electromagnetic hexapole or quadra/octa-pole was also applied ( Leary and Brydson, 2011 ). Eliminating these aberrations using correctors enhances the image quality of the focused beam ( Freitag et al, 2005 ).…”

Section: Technical Advancements In Cryo-em For High-resolution Struct...mentioning

confidence: 99%

Cryo-electron microscopy-based drug design

Cebi,

Lee,

Subramani

et al. 2024

Front. Mol. Biosci.

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Structure-based drug design (SBDD) has gained popularity owing to its ability to develop more potent drugs compared to conventional drug-discovery methods. The success of SBDD relies heavily on obtaining the three-dimensional structures of drug targets. X-ray crystallography is the primary method used for solving structures and aiding the SBDD workflow; however, it is not suitable for all targets. With the resolution revolution, enabling routine high-resolution reconstruction of structures, cryogenic electron microscopy (cryo-EM) has emerged as a promising alternative and has attracted increasing attention in SBDD. Cryo-EM offers various advantages over X-ray crystallography and can potentially replace X-ray crystallography in SBDD. To fully utilize cryo-EM in drug discovery, understanding the strengths and weaknesses of this technique and noting the key advancements in the field are crucial. This review provides an overview of the general workflow of cryo-EM in SBDD and highlights technical innovations that enable its application in drug design. Furthermore, the most recent achievements in the cryo-EM methodology for drug discovery are discussed, demonstrating the potential of this technique for advancing drug development. By understanding the capabilities and advancements of cryo-EM, researchers can leverage the benefits of designing more effective drugs. This review concludes with a discussion of the future perspectives of cryo-EM-based SBDD, emphasizing the role of this technique in driving innovations in drug discovery and development. The integration of cryo-EM into the drug design process holds great promise for accelerating the discovery of new and improved therapeutic agents to combat various diseases.

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Section: Technical Advancements In Cryo-em For High-resolution Struct...mentioning

confidence: 99%

Cryo-electron microscopy-based drug design

Cebi,

Lee,

Subramani

et al. 2024

Front. Mol. Biosci.

View full text Add to dashboard Cite

show abstract

“…But when C c correction is required, designers have no option but to use quadrupoles. Recent thinking about chromatic correction is to be found in Zach [34], Leary and Brydson [35] and Section 9.1 of [36] and, in connection with the SALVE and TEAM projects, in [37,38].…”

Section: Chromatic Correctionmentioning

confidence: 99%