2004
DOI: 10.1074/jbc.m410346200
|View full text |Cite
|
Sign up to set email alerts
|

Chromatin Domain Boundaries Delimited by a Histone-binding Protein in Yeast

Abstract: When located next to chromosomal elements such as telomeres, genes can be subjected to epigenetic silencing. In yeast, this is mediated by the propagation of the SIR proteins from telomeres toward more centromeric regions. Particular transcription factors can protect downstream genes from silencing when tethered between the gene and the telomere, and they may thus act as chromatin domain boundaries. Here we have studied one such transcription factor, CTF-1, that binds directly histone H3. A deletion mutagenesi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
34
0
1

Year Published

2005
2005
2025
2025

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 29 publications
(36 citation statements)
references
References 67 publications
1
34
0
1
Order By: Relevance
“…This observation is consistent with previous studies where CTF/NF1 proteins act as barrier elements that block the propagation of silent chromatin structures, and thus protect transgenes from silencing effects mediated by repressive chromatin structures. 31,32,43 Hence, CTF/NF1 binding sites act both as enhancer-blockers and as barrier insulator elements. Maintenance of a euchromatic status of the provirus should favor the production of retroviral vectors.…”
Section: Discussionmentioning
confidence: 99%
See 3 more Smart Citations
“…This observation is consistent with previous studies where CTF/NF1 proteins act as barrier elements that block the propagation of silent chromatin structures, and thus protect transgenes from silencing effects mediated by repressive chromatin structures. 31,32,43 Hence, CTF/NF1 binding sites act both as enhancer-blockers and as barrier insulator elements. Maintenance of a euchromatic status of the provirus should favor the production of retroviral vectors.…”
Section: Discussionmentioning
confidence: 99%
“…16,32 Nevertheless, whether these binding sites may also function as barrier elements upon transgene integration at internal chromosomal loci has not been assessed. The CTF/NF1 adeno (5-bp spacing) or CTCF nat binding sites were sub-cloned on each side of an SV40 promoter/GFP gene cassette (Figure 4a), to address the potential barrier properties of these sequences at random chromosomal locations.…”
Section: Ctf/nf1 As Insulators For Gene Transfer a Gaussin Et Almentioning
confidence: 99%
See 2 more Smart Citations
“…Une autre observation intéressante a été que le recrutement expérimental, entre les télomères et un gène rapporteur, de CTF1 (CCAAT-box-binding transcription factor 1), un facteur de transcription doté d'activité de remodelage de la chromatine, était capable de protéger le transgène de la répression transcriptionnelle induite par l'HC télomérique [17]. Des observations similaires avaient été faites au préalable chez la levure [18], suggérant à nouveau une conservation des mécanismes de régulation du TPE. La démonstration de l'existence d'un TPE sur des transgènes avait-elle la moindre signification physiologique ?…”
Section: L'effet De Position Télomérique Chez L'hommeunclassified