Chromatin plates in the interphase nucleus

Chicano, Andrea; Daban, Joan‐Ramon

doi:10.1002/1873-3468.13370

Cited by 7 publications

(18 citation statements)

References 70 publications

(126 reference statements)

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“…(bottom) represents schematically these two compaction states of planar chromatin; it shows part of a chromosome that is compacted (all layers are tightly stacked; panel D) in mitosis and early G1 phase, and more extended with few contacts between layers in late G1, S and G2 phases (panel E). This model is consistent with EM results indicating that the chromatin plates in interphase have a lower tendency to form stacked multilayer structures than chromatin from metaphase chromosomes .…”

Section: Regulation Of Gene Expression In Planar Chromatinsupporting

confidence: 91%

“…Chromatin emanated from disrupted nuclei suspended in buffers containing the cation concentrations found during interphase [18] also showed a laminar morphology [23]. Furthermore, chromatin fragments obtained from nuclei in G1, S and G2 cell cycle phases associate to form plate-like structures [23] in buffers containing physiological concentrations of monovalent cations and Mg 2+ (Fig. 1G, H).…”

Section: Edited By Claus Azzalinmentioning

confidence: 88%

“…The maintenance of the transverse orientation of chromosome bands after their mechanical splitting is also consistent with these observations. Since as indicated in the Introduction, there are evidences showing that chromatin plates do not disappear in interphase , these topological advantages are probably maintained throughout the cell cycle. In agreement with this, cytogenetic experiments using microdissection‐based multicolour banding showed that the band organization of chromosomes is essentially maintained during all the stages of the interphase (Fig.…”

Section: Looping and Topologically Associating Domains: Relationship mentioning

confidence: 90%

“…1G, H). The planar structures observed in interphase are not so well defined as the plates from metaphase chromosomes and show a low tendency to stack together [23].…”

Section: Edited By Claus Azzalinmentioning

confidence: 93%

“…Chromatin plates emanate from soft‐denatured metaphase chromosomes and interphase nuclei , and they can be self‐assembled from chromatin fragments in the presence of physiological concentrations of Mg 2+ . Figures reproduced with permission from the indicated references.…”

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confidence: 99%

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Supramolecular multilayer organization of chromosomes: possible functional roles of planar chromatin in gene expression and DNA replication and repair

Daban

2020

FEBS Letters

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Experimental evidence indicates that the chromatin filament is self‐organized into a multilayer planar structure that is densely stacked in metaphase and unstacked in interphase. This chromatin organization is unexpected, but it is shown that diverse supramolecular assemblies, including dinoflagellate chromosomes, are multilayered. The mechanical strength of planar chromatin protects the genome integrity, even when double‐strand breaks are produced. Here, it is hypothesized that the chromatin filament in the loops and topologically associating domains is folded within the thin layers of the multilaminar chromosomes. It is also proposed that multilayer chromatin has two states: inactive when layers are stacked and active when layers are unstacked. Importantly, the well‐defined topology of planar chromatin may facilitate DNA replication without entanglements and DNA repair by homologous recombination.

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Section: Regulation Of Gene Expression In Planar Chromatinsupporting

confidence: 91%

Section: Edited By Claus Azzalinmentioning

confidence: 88%

Section: Looping and Topologically Associating Domains: Relationship mentioning

confidence: 90%

“…1G, H). The planar structures observed in interphase are not so well defined as the plates from metaphase chromosomes and show a low tendency to stack together [23].…”

Section: Edited By Claus Azzalinmentioning

confidence: 93%

mentioning

confidence: 99%

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Supramolecular multilayer organization of chromosomes: possible functional roles of planar chromatin in gene expression and DNA replication and repair

Daban

2020

FEBS Letters

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Rethinking Models of DNA Organization in Micrometer‐Sized Chromosomes from the Perspective of the Nanoproperties of Chromatin Favoring a Multilayer Structure

Daban

2024

Small Structures

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The long genomic DNA molecules in eukaryotes are fragile and prone to entanglement, and must be tightly folded to fit into the micrometric dimensions of mitotic chromosomes. Histones transform the monotonous linear structure of double‐helical DNA into a chromatin filament formed by many nucleosomes. A physically consistent model for the packaging of the chromatin filament must be compatible with all the constraints imposed by the structural properties of chromosomes. It has to be compatible with 1) the high concentration of DNA and the elongated cylindrical shape of chromosomes and 2) the known self‐associative properties of chromatin, and also with 3) an effective protection of chromosomal DNA from topological entanglement and mechanical breakage. The multilayer chromosome model, in which a repetitive weak interaction between nucleosomes at the nanoscale produces the stacking of many chromatin layers, is compatible with all these constraints. The self‐organization of the multilayer structure of the whole chromosome is consistent with current knowledge of the self‐assembly of micrometric structures from different repetitive building blocks. The multilayer model justifies the geometry of chromosome bands and translocations, and is compatible with feasible physical mechanisms for the control of gene expression, and for DNA replication, repair, and segregation to daughter cells.

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