Chromatographic and electrophoretic methods for nanodisc purification and analysis

Justesen, Bo Højen; Pomorski, Thomas Günther

doi:10.1515/revac-2014-0014

Cited by 6 publications

(6 citation statements)

References 33 publications

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“…Free flow electrophoresis can also be used as a preparative or analytical tool, combining separation by size and charge. 146,170 Other methods include dynamic light scattering and analytical ultracentrifugation, 171 and also fluorescence correlation spectroscopy. 27…”

Section: Fundamental Structural Features Of Nanodiscsmentioning

confidence: 99%

“…Size exclusion chromatography is commonly used in protocols for the preparation of nanodiscs, as this simple method can provide a good estimate of size and polydispersity. Free flow electrophoresis can also be used as a preparative or analytical tool, combining separation by size and charge. , Other methods include dynamic light scattering and analytical ultracentrifugation, and also fluorescence correlation spectroscopy …”

Section: Structure and Assembly Of Nanodiscsmentioning

confidence: 99%

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Nanodiscs in Membrane Biochemistry and Biophysics

2017

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Membrane proteins play a most important part in metabolism, signaling, cell motility, transport, development, and many other biochemical and biophysical processes which constitute fundamentals of life on molecular level. Detailed understanding of these processes is necessary for the progress of life sciences and biomedical applications. Nanodiscs provide a new and powerful tool for a broad spectrum of biochemical and biophysical studies of membrane proteins and are commonly acknowledged as an optimal membrane mimetic system which provides control over size, composition and specific functional modifications on nanometer scale. In this review we attempted to combine a comprehensive list of various applications of Nanodisc technology with systematic analysis of the most attractive features of this system and advantages provided by Nanodiscs for structural and mechanistic studies of membrane proteins.

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Section: Fundamental Structural Features Of Nanodiscsmentioning

confidence: 99%

Section: Structure and Assembly Of Nanodiscsmentioning

confidence: 99%

Nanodiscs in Membrane Biochemistry and Biophysics

2017

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“…Historically, ND reviews have summarized the formation of nanodiscs, their structure, as well as the effect of nanodiscs on protein function . More recently, reviews have individually covered techniques that have been combined with ND technology, such as nuclear magnetic resonance (NMR) .…”

Section: Introduction To Nanodisc Technologymentioning

confidence: 99%

Recent advances in nanodisc technology for membrane protein studies (2012–2017)

et al. 2017

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Historically, progress in membrane protein research has been hindered due to solubility issues. The introduction of biomembrane mimetics has since stimulated the field’s momentum. One mimetic, the nanodisc, has proved to be an exceptional system for solubilizing membrane proteins. Herein, we critically evaluate the advantages and imperfections from employing nanodiscs in biophysical and biochemical studies. Specifically, we examine the techniques that have been modified to study membrane proteins in nanodiscs. Techniques discussed include fluorescence microscopy, solution state/solid state NMR, electron microscopy, SAXS, and several mass spectroscopy methods. Newer techniques such as SPR, charge sensitive optical detection, and scintillation proximity assays are also reviewed. Lastly, we cover nanodiscs advancing nanotechnology through nanoplasmonic biosensing, lipoprotein-nanoplatelets, and sortase-mediated labeling of nanodiscs.

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“…Thus, Nanodiscs are excellent biomimetic systems for studying the function of membrane proteins with complexes under experimental conditions. [ 15 ] We applied the synthesized TRPV1 nanodiscs to a GFET electrode to develop a TRPV1 activity‐based biomimetic system with a target reagent.…”

Section: Resultsmentioning

confidence: 99%

Reusable Electronic Tongue Based on Transient Receptor Potential Vanilloid 1 Nanodisc‐Conjugated Graphene Field‐Effect Transistor for a Spiciness‐Related Pain Evaluation

et al. 2023

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The sense of spiciness is related to the stimulation of vanilloid compounds contained in the foods. Although, the spiciness is commonly considered as the part of taste, it is more classified to the sense of pain stimulated on a tongue, namely, pungency, which is described as a tingling or burning on the tongue. Herein, first, a reusable electronic tongue based on a transient receptor potential vanilloid 1 (TRPV1) nanodisc conjugated graphene field‐effect transistor is fabricated and spiciness‐related pain evaluation with reusable electrode is demonstrated. The pungent compound reactive receptor TRPV1 is synthesized in the form of nanodiscs to maintain stability and reusability. The newly developed platform shows highly selective and sensitive performance toward each spiciness related vanilloid compound repeatably: 1 aM capsaicin, 10 aM dihydrocapsaicin, 1 fM piperine, 10 nM allicin, and 1 pM AITC. The binding mechanism is also examined by simulation. Furthermore, the elimination of the burning sensation on the tongue after eating spicy foods is not investigated. Based on the synthesis of micelles composed of casein protein (which is contained in skim milk) that remove pungent compounds bound to TRPV1 nanodisc, the deactivation of TRPV1 is investigated, and the electrode is reusable that mimics electronic tongue.

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Chromatographic and electrophoretic methods for nanodisc purification and analysis

Cited by 6 publications

References 33 publications

Nanodiscs in Membrane Biochemistry and Biophysics

Nanodiscs in Membrane Biochemistry and Biophysics

Recent advances in nanodisc technology for membrane protein studies (2012–2017)

Reusable Electronic Tongue Based on Transient Receptor Potential Vanilloid 1 Nanodisc‐Conjugated Graphene Field‐Effect Transistor for a Spiciness‐Related Pain Evaluation

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