Chromatographic and solid state nuclear magnetic resonance study of the changes in reversed-phase packings for high-performance liquid chromatography at different eluent compositions

Claessens, H.A.; Haan, J.W. de; Ven, L.J.M. van de; Bruyn, P.C. de; Cramers, C.A.M.G.

doi:10.1016/s0021-9673(00)94595-3

Cited by 36 publications

(17 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar conclusion was drawn in earlier pubications from this (2,3) and other ( 4 ) laboratories. Although hydrolysis and dissolution are rather slow processes in LC practice, the RP-HPLC phase deteriorates after a period of intensive use.…”

Section: Introductionsupporting

confidence: 90%

Influence of alkyl chain length on the stability of n-alkyl-modified reversed phases. 2. Model dissolution study

Hetem¹,

Haan²,

Claessens³

et al. 1990

Anal. Chem.

View full text Add to dashboard Cite

Document VersionPublisher's PDF, also known as Version of Record (includes final page, issue and volume numbers) Please check the document version of this publication:• A submitted manuscript is the author's version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website.• The final author version and the galley proof are versions of the publication after peer review.• The final published version features the final layout of the paper including the volume, issue and page numbers. G., & Kinkel, J. N. (1990). Influence of alkyl chain length on the stability of n-alkyl-modified reversed phases. 2. Model dissolution study. Analytical Chemistry, 62(21), 2296-2300. DOI: 10.1021/ac00220a008 Link to publicationGeneral rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal ? Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. INTRODUCTIONIn the foregoing, accompanying paper it is made clear that one of the most important reasons for instability of stationary phases for reversed-phase high-performance liquid chromatography (RP-HPLC) is concerned with unwanted chemical interactions between the mobile phase and the stationary phase ( I ) . A similar conclusion was drawn in earlier pubications from this (2,3) and other ( 4 ) laboratories. Although hydrolysis and dissolution are rather slow processes in LC practice, the RP-HPLC phase deteriorates after a period of intensive use. This is observed by changes in capacity factors, selectivity, and column efficiency.

show abstract

Section: Introductionsupporting

confidence: 90%

Influence of alkyl chain length on the stability of n-alkyl-modified reversed phases. 2. Model dissolution study

Hetem¹,

Haan²,

Claessens³

et al. 1990

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…After a chromatographic test to ensure reproducibility of the packing procedure six columns were placed in an apparatus for simulated routine use, while the remaining column was used as a reference column for initial chromatographic characterization. The equipment and procedure for simulated intensive routine use have been formerly described in detail [4]. The basic and acidic aqueous and methanol/aqueous buffers used for ageing were identical as before [6], see Table III.…”

Section: Chromatographymentioning

confidence: 99%

“…The effect of this treatment on the stability of modified octadecyl silane ligands is studied by ageing these phases under conditions which simulate routine use. Changes in properties after ageing are characterized and partially quantified as reported in earlier publications [4,6]. The two different octadecylsilane RP-HPLC stationary phases discussed here were prepared from a single starting silica substrate.…”

Section: Introductionmentioning

confidence: 99%

“…The long term stability of these stationary phases in laboratory practice is limited by hydrolysis of ligands from the surface and hydrolysis of the silica substrate [4][5][6]. The use of aqueous buffer solutions with pH values below 3 or above 8 and eluents with a relatively high salt content drastically decrease the useful lifetime of RP-HPLC columns [1,4,6].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of acid pretreatment of the silica substrate on the stability of octadecyl modified reversed phases

Hetem¹,

Haan²,

Claessens³

et al. 1990

Chromatographia

View full text Add to dashboard Cite

show abstract

“…of repeating units(n) No. of residues Net charge SEC+IEC IEC+RPC Ac-Gly-Gly-Gly-Leu-Gly-Gly-Ala-Gly-Gly-~u-Lys-amide Ac-Gly-Gly-Gly-~u-Gly-Gly-Ala-Gly-Gly-~u-Lys-amide Ac-Gly-Gly-Gly-Leu-Gly-Gly-Ala-Gly-Gly-Leu-Lys-aruide Ac-Gly-Gly-Gly-Leu-Gly-Gly-Ala-Gly-Gly-Leu-Lys-a~de The column performance can be tested by either chromatographic or non-chromatographic methods [21,22]; methods of the former group are usually preferable, as they provide more direct information on the chromatographic performance of the column and also on its changes on ageing.…”

Section: Approaches For Column Selection and Testingmentioning

confidence: 99%

Stationary phases for peptide analysis by high performance liquid chromatography: a review

Štulı́k¹,

Pacáková²,

Suchánková³

et al. 1997

Analytica Chimica Acta

View full text Add to dashboard Cite

A survey is given of modern stationary phases employed in high performance liquid chromatography (HPLC) analysis of peptides. The physico-chemical properties of peptides and their consequences for the selection and optimization of the separation system are briefly discussed, followed by a summary of the approaches to the selection and characterization of stationary phases. The properties and applicability of various stationary phases are then critically reviewed, including aspects such as size-exclusion, ion-exchange, reversed-phase, hydrophobic-interaction, affinity and chiral systems, as well as some specialized separation techniques. Emphasis is placed on the most recent literature. 0 1997 Elsevier Science B.V.

show abstract

Chromatographic and solid state nuclear magnetic resonance study of the changes in reversed-phase packings for high-performance liquid chromatography at different eluent compositions

Cited by 36 publications

References 89 publications

Influence of alkyl chain length on the stability of n-alkyl-modified reversed phases. 2. Model dissolution study

Influence of alkyl chain length on the stability of n-alkyl-modified reversed phases. 2. Model dissolution study

Effect of acid pretreatment of the silica substrate on the stability of octadecyl modified reversed phases

Stationary phases for peptide analysis by high performance liquid chromatography: a review

Contact Info

Product

Resources

About