Chromatographic Fingerprint Analysis and Characterization of Constituents in Shenqi Fuzheng Injection by HPLC-DAD-ELSD and UFLC-DAD-Q-TOF Tandem Mass Spectrometry Techniques

Abstract

Summary. Shenqi Fuzheng Injection (SFI) is a traditional Chinese medicine injection, widely used to enhance immune function of clinical cancer patients undergoing chemotherapy. In this study, a high-performance liquid chromatography-diode array detection-evaporative light scattering detection (HPLC-DAD-ELSD) method was established for quality control of SFI, which could simultaneously semiquantitatively reflect the constituents displayed in the chromatographic profile of SFI. The relative retention time and relative peak areas of the 21 common peaks related to the reference peak were calculated. The validity and advantage of this method were validated by systematically comparing chromatograms of 10 batches of SFI samples with the analytical methods of principal component analysis and angle cosine method recommended by the State Food and Drug Administration of China. Moreover, a total of 21 constituents of SFI were identified or tentatively characterized in the fingerprint via ultrafast liquid chromatography-diode array detection-quadrupole time-of-flight (UFLC-DAD-Q-TOF) tandem mass spectrometry technique on the basis of the retention time, ultraviolet spectra, fragmentation patterns, and reported literatures. All the results proved that the technique was useful in comprehensive quality evaluation of SFI and further study.

Identification and Pharmacokinetics of Multiple Potential Bioactive Constituents after Oral Administration of Radix Astragali on Cyclophosphamide-Induced Immunosuppression in Balb/c Mice

“…All compounds, except Peaks 15, 24, 25, 27, 28 and 39, have been reported in our previous studies [ 18 , 20 ]. By comparing the individual peak chromatographic behavior with those of reference standards, Peaks 15, 24 and 39 were unequivocally confirmed as hydroxylbenzoic acid, syringin and formononetin, respectively.…”

“…In recent decades, researchers have developed some methods to clarify the chemical basis for TCMs, such as fingerprint chromatography [ 26 ], serum pharmacology [ 27 ] and serum pharmacochemistry [ 28 ]. In the present research, serum pharmacochemistry was used based on our previous studies [ 14 , 18 , 20 ], trying to profile the potentially active constituents in RA by the ultra-fast liquid chromatography (UFLC)-DAD-Q-TOF-MS/MS technique.…”

“…The animal model of cyclophosphamide-induced immunosuppression in Balb/c mice in the present experiment has been published in previous reports [ 13 , 20 ]. Following randomization into study groups, mice (6/group/male) received intraperitoneal injection once daily of cyclophosphamide (i.p.…”

Antileukemic activity of the chemical constituents from Scutellaria barbata D. Don

“…The characteristic fragment ions and fragmentation pattern were summarized. Unknown compounds were identified by comparing with the information of isolated compounds and previously reported literatures [23][24][25]. The compounds in the S. barbata D. Don extract were Fig.…”

Quality evaluation of Shenmaidihuang Pills based on the chromatographic fingerprints and simultaneous determination of seven bioactive constituents

“…Chromatographic fingerprint analysis has been accepted as a strategy for quality assessment of herbal medicines and preparations by the WHO, the FDA, and China Food and Drug Administration (CFDA). It combines chromatographic techniques, such as CE , GC and HPLC , with sensitive and selective detectors like photodiode array detection (PDA) , evaporative light‐scatter detection (ELSD) and MS to construct specific patterns of recognition for multiple compounds in herbal drugs. The entire pattern of compounds can then be evaluated to determine not only the absence or presence of desired markers or actives but the complete set of ratios of all detectable analytes .…”