Chromatographic Fingerprinting and Quantitative Analysis of Marker in the Extract of Gloriosa superba Tubers Collected from Some Region of Chhattisgarh

Abstract

Background: Gloriosa superba (Family: Liliaceae) is commonly known as Kalihari in India and has been used by several indigenous communities to treat a snake bite, skin diseases and joint pain. It has been also scientifically reported for many pharmacological activities such as hypoglycaemic, hepatoprotective, anticancer, anti-inflammatory. Present work is an effort to develop validated HPTLC method for the detection and quantification of chief constituent in the tuber extract of Gloriosa superba. Methods: HPTLC analysis of tuber extract has been performed on Silica gel 60 F 254 (10 cm×10 cm) plates with mobile phase consisting toluene, ethyl acetate and diethylamine (02:08:02, v/v/v). Densitometric scanning of the plate was performed at 371nm by using CAMAG TLC scanner III equipped with visionCATS version 2.4.17207.2 (CAMAG) and developed method was also validated for accuracy, precision and robustness as per ICH guidelines. Results: present work has confirmed the rich content of colchicine in tuber extract of Gloriosa superba. The calibration curve was linear in the selected range of 0.4-1.2 µg/spot and regression equation found to be y = 0.0285x + 0.0074. the correlation coefficient (r) was 0.9978 for the regression equation. The LOQ and LOD was 0.170 µg /spot and 0.056 µg /spot respectively. The average recovery of colchicine at three levels was 99.5, 98.6 and 99.6 %, which indicated the remarkable reproducibility of the method. Conclusion: findings revealed that the developed method is simple, precise, and accurate for quantitative analysis of Gloriosa superba; and it might be useful for quality control of herbal medicine.