Chromatographic Method for Determination of the Amino Acid Content in Dioscorea bulbifera L. Tubers by RP-HPLC

Bhat, Musadiq Hussain; Fayaz, Mufida; Kumar, Amit; Dar, Alamgir A.; Jain, Ashok Kumar

doi:10.15171/ps.2019.10

Cited by 11 publications

(8 citation statements)

References 17 publications

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“…Its simplicity, ecofriendly and cost-effectiveness are the advantages of using GC instead of other techniques for the quantification of pesticides from natural sources. In continuation of our previous study (Bhat et al, 2019;Dangroo et al, 2014;Dangroo, Singh, et al, 2016;Dar et al, 2014Dar et al, , 2015Dar et al, , 2016Fayaz et al, 2018;Khan et al, 2013;Singh et al, 2018) on the application of chromatography for fingerprinting and chromatographic method development to assure the quality of vegetables/medicinal plants, a validated analytical method has been developed for the quantification of cypermethrin. Besides the development of a validated method, risk assessment and determination of the dissipation behaviour, persistence and half-life period of cypermethrin on green pea were carried out.…”

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confidence: 99%

Risk assessment, development and validation of a GC‐ECD‐based method for the quantification of cypermethrin from green pea

Jan

Dar

Wani

et al. 2022

Biomedical Chromatography

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The present work describes the persistence, dissipation behaviour, half‐life, risk assessment and novel gas chromatography method for the residue estimation of cypermethrin in green pea by spraying cypermethrin 10EC at 50 g a.i. ha−1 at fruiting stage followed by another application at a 10 day interval. The sample extraction and cleanup was followed bya modified quick, easy, cheap, effective, rugged, and safe method, and the residues of cypermethrin were determined using a validated gas chromatography method. The initial deposits were found to be 1.21 mg kg−1 following the application of insecticide at 50 g a.i. ha−1. Cypermethrin residues declined to below the detection limit of 0.05 mg kg−1 after 15 days at the recommended dosage. The half‐life of cypermethrin was 2.66 days at 50 g a.i. ha−1. For risk assessment studies, the waiting period of 15 days is recommended as safe for consumption for the insecticide. The GC‐ECD method was validated according to the SANTE guidelines by various analytical parameters including linearity, accuracy, detection and quantification limits. The developed method is simple, selective and repeatable, and can be used for the standardization of pesticides on fruits and vegetables.

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mentioning

confidence: 99%

Risk assessment, development and validation of a GC‐ECD‐based method for the quantification of cypermethrin from green pea

Jan

Dar

Wani

et al. 2022

Biomedical Chromatography

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“…27,[55][56][57] The instrumental methods of analysis are based upon the measurement of some physical properties of a substance to determine its chemical composition, quality, and quantification. 25,56,58 Presently, many sophisticated techniques have emerged, among which spectrophotometric and chromatographic techniques are being most widely used for the sample analysis. 48,59,60 The main objective of these methods is the analysis of the drug/marker which is of interest alone in the matrix of the excipients, additive, degradation products, impurities and so forth, and also other drugs in case of the combination products.…”

Section: Chromatographymentioning

confidence: 99%

Recent bioanalytical methods for the isolation of bioactive natural products from genus Codonopsis

Dar

Abrol

Singh

et al. 2023

Phytochemical Analysis

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Introduction Chromatography and spectroscopy are nowadays well‐validated techniques allowing to isolate and purify different class of natural products from the genus Codonopsis. Several categories of phytochemicals with drug like properties have been selectively extracted, isolated, characterised by this methodology. Objectives The present review aims to provide up‐to‐date and comprehensive information on the chromatography, phytochemistry and pharmacology of natural products of Codonopsis with an emphasis on the search for natural products having various biological activities and the semi‐synthetic derivatives of bioactive ones and to highlight current gaps in knowledge. Materials and methods A literature search was performed in the SciFinder Scholar, PubMed, Medline, and Scopus databases. Results During the period covered in this review, several classes of compounds have been reported from genus Codonopsis. Codonopsis pilosula and Codonopsis lanceolata are the most popular in the genus especially as per phytochemical and bioactive studies. Phytochemical investigation demonstrates that Codonopsis species contain mainly xanthones, flavonoids, alkaloids, polyacetylenes, phenylpropanoids, triterpenoids and polysaccharides, which contribute to numerous bioactivities. The major bioactive compounds isolated were used for semi‐synthetic modification to increase the chance to discover lead compound. Conclusions It can be concluded that genus Codonopsis has been used as traditional medicines and food materials around the world over years due to chemical constituents with diverse structural types, exhibiting extensive pharmacological activities in immune system, blood system, cardiovascular system, central nervous system, digestive system, and so forth, with almost no obvious toxicity and side effect. Therefore, Codonopsis can be used as a promising ethnopharmacological plant source.

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“…The amount of total free amino acids was estimated using a standard graph and expressed in %. Individual amino acid profiling was done using RP HPLC method (Bhat et al, 2019).…”

Section: Total and Individual Amino Acid Profilingmentioning

confidence: 99%

Nutritional prospective of Sesbania species: an underutilized wild legume from Northern Western Ghats, Maharashtra, India

Patil

2022

Acta fytotechn zootechn

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Licensed under a Creative Commons Attribution 4.0 International License Nutrition plays very important role in longevity of life. Processed food has created various health disorders at a very alarming rate. Wild genotypes have potent nutritive characteristics which are relatively less explored. In the current investigation, two species of Sesbania were evaluated for their potent nutritional value. Sesbania grandiflora and Sesbania bispinosa seeds contained 37.78% and 30.2% crude proteins, 4.24% and 2.06% albumins, 7.20% and 5.16% globulins, 1.75% and 8.34% free amino acids, 34.91% and 59.08% total carbohydrates and 5.2% and 5.02% total lipids respectively. DPPH Radical scavenging assay revealed notable antioxidant potential with 62.27% and 65.45% inhibition respectively. Antinutrient analysis indicated the presence of total free phenols (0.29% and 0.63%), tannins (0.28% and 0.34%), phytic acid (1.70% and 1.59%) and trypsin inhibitor activity (41.8 and 54.4 TIU mg -1 ) respectively. Both Sesbania species had excellent nutritional potential as well as antioxidant activity and a low antinutrient profile. Based on the findings, Sesbania species may be investigated further as a potential alternative food source in the near future, particularly in economically disadvantaged areas, and may pave the way toward the wider aim of food security.

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Chromatographic Method for Determination of the Amino Acid Content in Dioscorea bulbifera L. Tubers by RP-HPLC

Cited by 11 publications

References 17 publications

Risk assessment, development and validation of a GC‐ECD‐based method for the quantification of cypermethrin from green pea

Risk assessment, development and validation of a GC‐ECD‐based method for the quantification of cypermethrin from green pea

Recent bioanalytical methods for the isolation of bioactive natural products from genus Codonopsis

Nutritional prospective of Sesbania species: an underutilized wild legume from Northern Western Ghats, Maharashtra, India

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