1987
DOI: 10.1002/bmc.1130020602
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Chromatographic methods for bile acid analysis

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Cited by 32 publications
(14 citation statements)
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“…The derivatization of bile acids often involved two major reactions, the esterification of the carboxyl group and ether formation of the hydroxyl group as mentioned previously. Methyl, ethyl, n-propyl, n-butyl and isobutyl esters all had been used in the derivatization of carboxylic group for the GC analysis of bile acids [28,29]. Marschall et al [30] compared three different methods for derivatization: (i) diazomethane in diethyl ether, (ii) trimethylsilyldiazomethane in toluene and (iii) concentrated hydrochloric acid in dimethoxypropane for methylation of bile acids in GC-MS analysis.…”
Section: Optimization Of the Derivatization Condition For Bile Acidsmentioning
confidence: 99%
“…The derivatization of bile acids often involved two major reactions, the esterification of the carboxyl group and ether formation of the hydroxyl group as mentioned previously. Methyl, ethyl, n-propyl, n-butyl and isobutyl esters all had been used in the derivatization of carboxylic group for the GC analysis of bile acids [28,29]. Marschall et al [30] compared three different methods for derivatization: (i) diazomethane in diethyl ether, (ii) trimethylsilyldiazomethane in toluene and (iii) concentrated hydrochloric acid in dimethoxypropane for methylation of bile acids in GC-MS analysis.…”
Section: Optimization Of the Derivatization Condition For Bile Acidsmentioning
confidence: 99%
“…A portion of each bile extract was subjected to HPLC analysis (18 Typical negative-ion FAB-MS spectra obtained from the bile of CF patients with associated liver disease, before (top spectrum) and after (bottom spectrum) UDCA administration. The following cholanoic acid structures are indicated from the ions: m/z 432 (glycomonohydroxy), m/z 448 (glyco-dihydroxy), m/z 464 (glyco-trihydroxy), m/z 480 (glyco-tetrahydroxy), m/z 498 (tauro-dihydroxy), m/z 514 (tauro-hydroxy), m/z 528 (glyco-dihydroxy-sulfate).…”
Section: Fast Atom Bombardment-mass Spectrometry Of Intactmentioning
confidence: 99%
“…There have been a number of reports on the liquid chromatographic separation, identification and quantification of bile acids in biological fluids (Swobodnik et al., 1987;Hasegawa et al, 1984;Li et al, 1989;Street and Setchell, 1989;Zhang et al, 1992;Chen ef al., 1992). At present, reversed phase high performance liquid chromatography (RP-HPLC) is the method of choice for the assay of free and conjugated bile acids in biological samples at elevated levels.…”
Section: Introductionmentioning
confidence: 97%