Chromatographic Study on the Essential Oil of Tetraclinis Articulata Vahl Masters from Morocco

Abstract

With a view to contributing to the evaluation of Moroccan flora through the identification of new potentially interesting at a biological and therapeutic level, we have indertaken a study of the essential oil in the sawdust wood of the Tetraclinis articulata Vahl Masters from the Khemisset region (north-west Morocco). The study of the sawdust's essential oil, completed by GC and GC/MS, identified 22 components, the main five being: α-acorénol (20.9%), cédrol (17.9%), totarol (8.8%), α-cédrène (8.7%) and β-acorénol (7.4%). This essential oil was fractionated on an open silica column using an eluent of increasing polarity. Five fractions were collected in this way.The fraction (FH) eluted with the pentane comprised sesquiterpene hydrocarbons whereas the four oxygenated fractions (FO1 to FO4) eluted with a diethyl oxide/pentane mix of increasing polarity were dominated by a diterpene phenol and two sesquiterpene alcohols respectively: totarol in the FO1 (42.4%), α-acorénol in the FO2 (34.9%) and FO3 (54.0%), and cédrol in the FO4 (58.2%). The fractionation of the oil has allowed us to concentrate constituents likely to be valued for their biological and organoleptic properties.

Preservation of poultry meat using Tetraclinis articulata essential oil during refrigerated storage

“…Monoterpene hydrocarbons (51.63%) represented the main fraction of Tetraclinis articulata EO where α-pinene (36.47%) and D-limonene (5.38%) were the main components of this fraction. These results were in accordance with those determined by Bourkhiss et al (2007) who detected lower contents of α-pinene (30.22%) and D-limonene (22.29%) in Tetraclins articulata EO in Morocco. Regarding sesquiterpenes class, δ-elemene (15.14%), trans-caryophyllene (2.81%) and germacrene-D (2.48%) constituted the main components (Table 1).…”

“…The extraction of Tetraclinis articulata EO showed a yield value of 0.44%. Our results were remarkably higher in comparison to those reported in the literature where the extraction yields of Tetraclinis articulata harvested from Algeria and Morocco regions were respectively 0.17% and 0.22% (w/w) (Bourkhiss et al, 2007; Boussaïd et al, 2015). This difference could be due to many factors notably geo-climatic, the origin, the organ of the plant as well as the drying time (Şanli and Karadoğan, 2016).…”

“…This difference could be due to many factors notably geo-climatic, the origin, the organ of the plant as well as the drying time (Şanli and Karadoğan, 2016). On the other hand, the use of ultrasound for the sono-clevenger could improve the extraction and allowed rapid and complete recovery of the matrix to obtain better yields (Pingret et al, 2014). Ultrasonic extraction has been proven to achieve higher extraction yields and reduce energy consumption and time of extraction (Nora and Borges, 2017).…”