Chromatographic techniques: types, principles, and applications

Kumari, V. B. Chandana; Patil, Shashank M.; Ramu, Ramith; Shirahatti, Prithvi S.; Kumar, Naveen; Sowmya, B.; Egbuna, Chukwuebuka; Uche, Chukwuemelie Zedech; Patrick-Iwuanyanwu, Kingsley C.

doi:10.1016/b978-0-12-822654-4.00013-0

Cited by 13 publications

(4 citation statements)

References 96 publications

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“…Column chromatography is used to separate impurities and purify biological mixtures. It is also used to isolate active molecules and extract metabolites from various samples [157]. The solid and liquid samples can be separated and purified by this method.…”

Section: Column Chromatographymentioning

confidence: 99%

Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

Susanti,

Pratiwi,

Rosandi

et al. 2024

Plants

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In recent years, discovering new drug candidates has become a top priority in research. Natural products have proven to be a promising source for such discoveries as many researchers have successfully isolated bioactive compounds with various activities that show potential as drug candidates. Among these compounds, phenolic compounds have been frequently isolated due to their many biological activities, including their role as antioxidants, making them candidates for treating diseases related to oxidative stress. The isolation method is essential, and researchers have sought to find effective procedures that maximize the purity and yield of bioactive compounds. This review aims to provide information on the isolation or separation methods for phenolic compounds with antioxidant activities using column chromatography, medium-pressure liquid chromatography, high-performance liquid chromatography, counter-current chromatography, hydrophilic interaction chromatography, supercritical fluid chromatography, molecularly imprinted technologies, and high-performance thin layer chromatography. For isolation or purification, the molecularly imprinted technologies represent a more accessible and more efficient procedure because they can be applied directly to the extract to reduce the complicated isolation process. However, it still requires further development and refinement.

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Section: Column Chromatographymentioning

confidence: 99%

Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

Susanti,

Pratiwi,

Rosandi

et al. 2024

Plants

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“…Paper chromatography, invented by Martin and Synge in 1943, can be considered the first paper-based sensor . Later, in the 1970s, the first LF test was commercialized: the pregnancy test, which is one of the most used POCTs. , Following this milestone, the first infectious disease LF test was commercialized in the late 1980s for the detection of Group A Streptococcus pyogenes from throat swab samples .…”

Section: Lateral Flowmentioning

confidence: 99%

Toward Integrated Molecular Lateral Flow Diagnostic Tests Using Advanced Micro- and Nanotechnology

2022

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“…this separation technique is the difference between compounds in terms of their interaction with two phases, one fixed stationary phase and another moving mobile phase (Tarafder and Miller, 2021;Kumari et al, 2022). Chromatography is classified based on the type of the stationary and mobile phases used as well as the separation apparatus working mode.…”

Section: Main Principles For the Separation And Identification Of Dif...mentioning

confidence: 99%

Chromatographic Techniques to Separate and Identify Bioactive Compounds in Microalgae

et al. 2022

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Microalgae are potential sources for the sustainable production of valuable chemicals including polyphenols, pigments, and ω-3 PUFAs. However, successful exploitation of these high value compounds in the food, healthcare and pharmaceutical sectors depends greatly on their effective separation, identification, and analysis after recovery from the biomass. The findings of this review paper illustrated that chromatographic methods coupled to different types of detectors have been used as a crucial part of research on microalgal polyphenols, Omega-3 Polyunsaturated Fatty Acids (ω-3 PUFAs), and pigments production through identification, measurement, sample preparation, and purification practices. Therefore, it is important to provide a comprehensive review regarding the current research in the field. The basic operating principles, parametric optimisation and detection units of common (liquid chromatography and gas chromatography) and novel chromatographic techniques (counter current chromatography, expanded bed adsorption chromatography and supercritical fluid chromatography) used to separate, identify, and quantify polyphenols, PUFAs and pigments from microalgae matrices are comprehensively reviewed.

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Chromatographic techniques: types, principles, and applications

Cited by 13 publications

References 96 publications

Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

Toward Integrated Molecular Lateral Flow Diagnostic Tests Using Advanced Micro- and Nanotechnology

Chromatographic Techniques to Separate and Identify Bioactive Compounds in Microalgae

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