Chromatography: Separation Techniques in Biology

Lough, W. J.; Carlile, Mark

doi:10.1002/9781119483977.ch7

Cited by 1 publication

(1 citation statement)

References 110 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liquid chromatography-mass spectrometry (LC-MS) and liquid chromatography with tandem mass spectrometry (LC-MS/MS) involve the chromatographic separation of analytes followed by the detection of their m/z value. With the help of high-performance liquid chromatography or ultra-performance liquid chromatography, the complexity of analytes extracted from the biological samples is effectively reduced and more analytes can be detected by MS ( 56 ). To provide a broad coverage of molecules with different chemical and physical properties, different chromatographic platforms are developed, including reversed-phase chromatography, hydrophobic-interaction chromatography and ion exchange chromatography ( 57 – 59 ).…”

Section: Introductionmentioning

confidence: 99%

Applications of spatially resolved omics in the field of endocrine tumors

et al. 2023

View full text Add to dashboard Cite

Endocrine tumors derive from endocrine cells with high heterogeneity in function, structure and embryology, and are characteristic of a marked diversity and tissue heterogeneity. There are still challenges in analyzing the molecular alternations within the heterogeneous microenvironment for endocrine tumors. Recently, several proteomic, lipidomic and metabolomic platforms have been applied to the analysis of endocrine tumors to explore the cellular and molecular mechanisms of tumor genesis, progression and metastasis. In this review, we provide a comprehensive overview of spatially resolved proteomics, lipidomics and metabolomics guided by mass spectrometry imaging and spatially resolved microproteomics directed by microextraction and tandem mass spectrometry. In this regard, we will discuss different mass spectrometry imaging techniques, including secondary ion mass spectrometry, matrix-assisted laser desorption/ionization and desorption electrospray ionization. Additionally, we will highlight microextraction approaches such as laser capture microdissection and liquid microjunction extraction. With these methods, proteins can be extracted precisely from specific regions of the endocrine tumor. Finally, we compare applications of proteomic, lipidomic and metabolomic platforms in the field of endocrine tumors and outline their potentials in elucidating cellular and molecular processes involved in endocrine tumors.

show abstract

Section: Introductionmentioning

confidence: 99%