Chromatography with a two-dimensional column

Role of chromatographic techniques in proteomic analysis

“…A paper chromatography performed in two dimensions is an example. In terms of peak capacity, this system has a resolving power of several hundreds [47,48]. Even though two-dimensional column systems to further enhance peak capacity were proposed at that time, they did not surpass other two-dimensional separation techniques.…”

Construction and initial evaluation of an apparatus for spatial comprehensive two-dimensional liquid-phase separations

“…Two-dimensional thin-layer chromatography (2D-TLC) is a form of spatial chromatography. A generalization of the concept has been published and patented [25,26]. However, the practical implementations have thus far been quite limited [27,28].…”

“…Here, the mobile phase does not flow due to capillary forces under atmospheric conditions, as in conventional TLC. Instead, a closed bed compartment and a pump are used in such a way that the mobile-phase velocity is kept constant [25]. Two-dimensional gel electrophoresis (2-DE) is another example of two-dimensional spatial separation.…”

Two-Dimensional Separation Processes

“…Computer analysis of the data will be required. Up to 900 peaks should be separable with this apparatus, but no experimental results were presented (6,7). A theoretical investigation of both two-and three-dimensional columns has been presented (7).…”

“…In applying this to two-dimensional systems, the solutes are retained in the system during the first development and eluted during the second development (6,7). A twedimensional column system using a 1024 pixel array of diodes as the detector is shown schematically in Fig.…”