Chromogranin A: a surprising link between granule biogenesis and hypertension

Kim, Taeyoon; Loh, Y. Peng

doi:10.1172/jci25706

Cited by 34 publications

(29 citation statements)

References 19 publications

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“…The novel fragment of CgA termed catestatin is a potent inhibitor of CA release, acting as a non-competitive antagonist on the nicotinic cholinergic receptor. CgA, catestatin and CA are co-released into the extra-cellular environment [15]. CA mediates sympathoadrenal activity on cardiovascular target cells to increase blood pressure.…”

Section: Introductionmentioning

confidence: 99%

Relationship between salivary Chromogranin-A and stress induced by simulated monotonous driving

Yamakoshi

Park

Jang

et al. 2009

Med Biol Eng Comput

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Abstract:The purpose of the present study was to evaluate the use of salivary Chromogranin-A (CgA), which is already used in general as a mental stress marker, for studying the stressful situation created by simulated monotonous driving. After informed consent 25 healthy male & female subjects were studied, under constant environment-controlled conditions. We measured the following physiological variables: blood pressure (BP), cardiac output (CO), total peripheral resistance (TPR), normalized pulse volume (NPV) as an index of alpha-adrenergic sympathetic activity to the finger arteriolar vessels, levels of cortisol and CgA during monotonous driving. The induced stress led to the expected decreases in NPV and increases in TPR and BP caused by peripherally-related sympathetic acceleration. However, CgA levels were found to fall gradually in accordance with the gradual increase of subjective rating of stress (SRS) and significantly (p<0.01) decreased over the period of the simulated monotonous driving.Our hypothesis for the gradual decrease of CgA levels during the simulated monotonous driving is as follows. CgA, catestatin and Catecholamines are co-released into the extra-cellular environment. Peripheral sympathetic activity was accelerated by stress resulting from the simulated monotonous driving. Upon peripheral vessel constriction, an increase in total peripheral resistance (TPR) then increased BP which, in turn, activated catestatin. Consequently, secretion of CgA was blocked by the co-secreted catestatin from chromaffin granules. The results obtained strongly indicate that, although CgA has been reported as a possible marker of stress, CgA levels are not increased in the stressful situation of simulated monotonous driving.

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Section: Introductionmentioning

confidence: 99%

Relationship between salivary Chromogranin-A and stress induced by simulated monotonous driving

Yamakoshi

Park

Jang

et al. 2009

Med Biol Eng Comput

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“…Granins are involved in granulogenesis in neuroendocrine cells (6,7) but there has been no published analysis of expression or function of granins in the mast cell system. Our data demonstrate the expression of granins in mast cells, and imply an important role for these proteins in this secretory immunocyte.…”

Section: Discussionmentioning

confidence: 99%

“…The molecular functions of the granins are poorly understood. There is evidence that granins may be the precursors of biologically active peptides (3,(5)(6)(7)(8)(9). However, granins cannot be considered simply as precursors of granule cargo.…”

mentioning

confidence: 99%

“…However, granins cannot be considered simply as precursors of granule cargo. They may act as helper proteins in the packaging of peptide hormones and neuro-peptides (3,(5)(6)(7)(8)(9). The EF-hand containing CgA and CgB may also act as high capacity, low affinity, calcium binding proteins that are closely associated with calcium-regulated intracellular ion channels (10 -13).…”

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confidence: 99%

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Secretogranin III Directs Secretory Vesicle Biogenesis in Mast Cells in a Manner Dependent upon Interaction with Chromogranin A

Prasad

Yanagihara

Small‐Howard

et al. 2008

The Journal of Immunology

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Mast cells are granular immunocytes that reside in the body’s barrier tissues. These cells orchestrate inflammatory responses. Proinflammatory mediators are stored in granular structures within the mast cell cytosol. Control of mast cell granule exocytosis is a major therapeutic goal for allergic and inflammatory diseases. However, the proteins that control granule biogenesis and abundance in mast cells have not been elucidated. In neuroendocrine cells, whose dense core granules are strikingly similar to mast cell granules, granin proteins regulate granulogenesis. Our studies suggest that the Secretogranin III (SgIII) protein is involved in secretory granule biogenesis in mast cells. SgIII is abundant in mast cells, and is organized into vesicular structures. Our results show that over-expression of SgIII in mast cells is sufficient to cause an expansion of a granular compartment in these cells. These novel granules store inflammatory mediators that are released in response to physiological stimuli, indicating that they function as bona fide secretory vesicles. In mast cells, as in neuroendocrine cells, we show that SgIII is complexed with Chromogranin A (CgA). CgA is granulogenic when complexed with SgIII. Our data show that a novel non-granulogenic truncation mutant of SgIII (1–210) lacks the ability to interact with CgA. Thus, in mast cells, a CgA-SgIII complex may play a key role in secretory granule biogenesis. SgIII function in mast cells is unlikely to be limited to its partnership with CgA, as our interaction trap analysis suggests that SgIII has multiple binding partners, including the mast cell ion channel TRPA1.

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“…Chromogranin A (CgA), a major member of the granin family of acidic secretory glycoproteins, is known to be released from the adrenal medulla into the blood with catecholamine (Kim, 2005). Therefore it is considered to be a possible biomarker of SAM system (Nakane, 1999;Winkler, 1992).…”

Section: Salivary Cgamentioning

confidence: 99%

Salivary Hormones, Immunes and Other Secretory Substances as Possible Stress Biomarker

Nomura¹

2012

Biomarker

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Chromogranin A: a surprising link between granule biogenesis and hypertension

Cited by 34 publications

References 19 publications

Relationship between salivary Chromogranin-A and stress induced by simulated monotonous driving

Relationship between salivary Chromogranin-A and stress induced by simulated monotonous driving

Secretogranin III Directs Secretory Vesicle Biogenesis in Mast Cells in a Manner Dependent upon Interaction with Chromogranin A

Salivary Hormones, Immunes and Other Secretory Substances as Possible Stress Biomarker

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