Chromosomal aberrations by fluorescence in situ hybridization (FISH)—Biomarker of exposure to carcinogenic PAHs

Frequency of chromosomal aberrations in Prague mothers and their newborns

“…In most of them an effect of environmental pollutants on the levels of aberrations was detected: in city policemen from Prague, Czech Republic, the levels of F G /100 decreased significantly along with the drop in exposure to c-PAHs in different seasons [4]. Similarly, in police officers from Prague and Sofia, Bulgaria, the levels of stable aberrations were significantly higher than in controls exposed to lower air pollution measured by personal monitors [3]. On the other hand, Pedersen et al [28] did not find any difference between groups of adults and children residing in two regions of the Czech Republic, probably due to their unusually low exposure to pollutants 1 month before the sampling period.…”

“…Our results indicate that exposure to above-median levels of c-PAHs was not accompanied by increased frequencies of either type of aberration in either group, probably due to the very narrow range of c-PAH concentrations during the study months. Several studies analyzing the effect of environmental exposures on the levels of stable and unstable aberrations have been published [3,4,16,17,28]. In most of them an effect of environmental pollutants on the levels of aberrations was detected: in city policemen from Prague, Czech Republic, the levels of F G /100 decreased significantly along with the drop in exposure to c-PAHs in different seasons [4].…”

“…According to results by Binkova et al [2] the genotoxicity of c-PAHs adsorbed onto airborne particles poses the most significant health risk from air pollution in the Czech Republic. Environmental exposure to c-PAHs has been associated with an increase in the genomic frequency of translocations in peripheral lymphocytes [3,4]. Recent studies have focused on the most polluted and urbanized places around the world [5][6][7].…”

Impact des facteurs individuels et environnementaux sur le taux d’aberrations chromosomiques de type translocations Partie 1 : âge, sexe, tabac, alcool

“…Sur les 10 publications qui étudient l'impact du tabac sur le nombre de translocations, sept ne montrent pas d'augmentation significative du taux de translocations après une consommation excessive de tabac (plus de 20 cigarettes par jour) (Beskid et al, 2007 ;Maeng et al, 2004 ;Pressl et al, 1999 ;Tawn et Cartmell, 1989 ;Tucker et al, 2003 ;Van Diemen et al, 1995 ;Whitehouse et al, 2005). La consommation de cigarettes par jour varie selon le terme employé.…”

“…Et ainsi augmenter la spécificité de la dosimétrie biologique rétrospective. 35 Burim, 2004Badr, 1982Ramsey, 1995Pressl, 1999Bothwell, 2000Tucker, 2003Beskid, 2007 Van Diemen, Tawn et Carmell, 1989Sigurdson et al, 2008 Comparison between the translocation rate generated by different factors and that generated by irradiation in vitro.…”