Chromosomal sublocalization and microsatellite DNA polymorphism of human adrenomedullin gene

Plasma adrenomedullin level is related to a single nucleotide polymorphism in the adrenomedullin gene

“…These previous studies showed significant associations of SNPs or a microsatellite marker of cytosine-adenine (CA) repeat located at the 3 0 -end of ADM with essential hypertension, renal diseases, and dysglycemia (30,(38)(39)(40)(41)(42). However, most of these studies have not examined the association of these genetic variants with plasma ADM. Only two studies have investigated the effect of the microsatellite marker of CA repeat on plasma ADM, but the results of both the studies were negative (39,40). Our study demonstrated for the first time that the common SNP rs4910118, with a MAF of 23.4%, was associated with plasma ADM in Chinese (Hong Kong).…”

Microsatellite DNA polymorphism of human adrenomedullin gene in type 2 diabetic patients with renal failure

“…The number of CA repeats adjacent to AM gene was RESULTS determined by fluorescence-labeled polymerase chain Clinical characteristics of study subjects reaction and polyacrylamide gel electrophoresis (PCR-PAGE) as described previously [12,17]. Genomic DNA Table 1 lists the physical and laboratory findings of was extracted from peripheral leukocytes using Easy the study subjects.…”

“…These allele frequencies were not significantly different in DM-C and CGN-HD. However, adenine (CA) repeat [12]. This microsatellite marker is DM-HD showed significantly different distribution of allele located approximately 4 kb downstream of the AM gene.…”

Variations of Human Adrenomedullin Gene and Its Relation to Cardiovascular Diseases

“…Nucleotide sequencing of genomic DNA adjacent to the human AM gene revealed that there is a microsatellite marker with a variable number of cytosine adenine (CA) repeats at 4 kb downstream from the 3 -end of the AM gene (8,40). We investigated the relation of this microsatellite DNA polymorphism flanking to the 3 -end of the AM gene with genetic predispositions to develop various cardiovascular diseases.…”

“…It was thus determined that the human AM gene is localized on chromosome 11. Then, we carried out further chromosomal sublocalization of the human AM gene using the fluorescence in situ hybridization (FISH) technique (8). The cultured human lymphocytes treated with 5 -bromo-2 deoxyuridine (BrdU) for synchronization were fixed on slides and hybridized with a fluorescence-labeled genomic DNA fragment encoding the full-length human AM gene.…”