Chromosome abnormalities in non‐small cell lung cancer pleural effusions: Cytogenetic indicators of disease subgroups

Abstract

A cytogenetic study of pleural effusions (PE) containing metastatic or invasive tumor cells from 11 patients with non-small cell lung cancer (NSCLC) (3 squamous cell carcinomas [SQC] and 8 adenocarcinomas [ADC] including 1 giant cell variant) was performed to identify non-random chromosome abnormalities. Numerical abnormalities seen in > or = 30% of cases included gain of chromosomes 7 and 20, and loss of chromosomes 4, 9, 10, 13, 15, 16, 18, 19, 21, and 22. The most frequent structural abnormality involved rearrangement in 1p with breakpoints clustering at 1p10-p13. Other recurrent breakpoint regions, seen in > or = 30% of cases, occurred in chromosome region 3p10-p21, 3q11-q25, 6p11-p25, 6q13-q23, 7q11-q36, 9q32-q34, 11p11-p13, 11q13-q24, 13p/14p and/or 15p, 17p and 19p, with, in particular, apparent loss of 6q21-q27, 3p21-p26, 7q21-q22, 9p22-p24 (shortest regions of common overlap) and 17p. There was also recurrent gain of 1q23-q44, 8q13-q24, and 11q13-q23. These abnormalities were not restricted to a particular histological subtype, with the exception of +8 and a breakpoint in 9q32-q34, which were seen only in ADC. The 9q32-q34 breakpoint observed in 4 ADC PE (including 1 giant cell variant) represents a new observation in NSCLC. These findings, when compared to those reported for primary NSCLC indicate cytogenetic differences between the two which may be associated with pleural invasion of NSCLC.

Comparison of DNA copy number changes in malignant mesothelioma, adenocarcinoma and large-cell anaplastic carcinoma of the lung

“…Gains of genetic material in the long arm of chromosome 8 are not often found in cytogenetic analyses. However, the presence of isochromosome 8q has been associated with primary adenocarcinomas (Jin et al, 1988) and gains in 8q have been reported to be frequent in pleural effusions from NSCLC patients (Lukeis et al, 1993). In our CGH analysis, this particular aberration (including three high-level amplifications) occurred in 65% of the informative tumours.…”

Allelic imbalance and instability of microsatellite loci on chromosome 1p in human non-small-cell lung cancer

“…The terms 'allelic imbalance', 'loss of heterozygosity' and 'allelespecific deletions' are used equally because cytogenetic studies have rarely shown lp amplification in NSCLC, whereas deletions have been found (Testa and Siegfried, 1992;Lukeis et al, 1993). Furthermore, gene LMYC (lp32) is rarely amplified (< 1%) in these tumours (Yokota et al?…”

“…Cytogenetic analysis has shown frequent structural rearrangements at lpl3 (Testa and Siegfried, 1992;Lukeis et al, 1993;Johansson et al, 1994). Balanced translocations seemed to be relatively rare in NSCLC.…”

“…Cytogenetic analysis of NSCLC has shown a consistent deletion at lpl3 (Testa and Siegfried, 1992;Lukeis et al, 1993;Johansson et al, 1994), although the use of cytogenetic analysis is complicated for these tumours because of a low mitotic index and extremely complex karyotypes with many additional chromosomes (Testa and Siegfried, 1992).…”

A novel epithelial-expressed ETS gene, ELF3: human and murine cDNA sequences, murine genomic organization, human mapping to 1q32.2 and expression in tissues and cancer

“…Cell lines PL27 and L162 (Lukeis et al, 1993) were obtained from Dr S Hasthorpe (Royal Childrens Hospital, Melbourne) and maintained as for COS cells. Other cell lines were obtained from ATCC.…”