Chromosome Abnormalities in Rabbit Blastocysts Following Delayed Fertilization

Shaver, Evelyn L.; Carr, David

doi:10.1530/jrf.0.0140415

Cited by 81 publications

(28 citation statements)

References 14 publications

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“…However, in agreement with Shaver & Carr (1967, 1969, 8 h delay gave a relatively high yield of triploids (3-13%), though not so high as reported by them (12-5% after 6-9 h delay).…”

Section: (Iii) Properties Of the M-markersupporting

confidence: 87%

“…In the rabbit, the M-marker segregations showed that nearly all triploidy after 8 h delayed insemination was digynic. The 12 XXX and 24 XXY were in a proportion deviating significantly from the digynic expectation of 50:50 at the time of fertilization (Shaver & Carr (1967,1969 report 3 XXX, 7 XXY after 6-9 h delay). This suggests a higher relative viability of XXY, but a detailed quantitative check on the lines of Beatty (1978) cannot be made until more data are available.…”

Section: (V) the Relative Viability Of XXX And Xx Y Triploidsmentioning

confidence: 75%

“…In non-experimental rabbits not subjected to superovulation, there were 4 3N and 665 non-3N, or 0-60% 3N (0, 18 (Hofsaess & Meacham, 1971); 0, 105 (Martin & Shaver, 1972); 1, 71 (Widmeyer & Shaver, 1972);0,196 (Martin-DeLeon, 1972); 0, 75 (Shaver, 1970); 3, 69 (Fujimoto, Pahlavan & Dukelow, 1974); 0, 131 (Shaver & Carr, 1967, 1969). The same category of rabbits, but after superovulation, yielded 2 3N and 344 non-3N, or 0-58 % 3N (0, 36 (Fujimoto et al 1974); 2, 265 (Fechheimer & Beatty, 1974 'fresh semen'); 0, 43 (present work)).…”

Section: Introductionmentioning

confidence: 99%

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Digynic triploidy in rabbit blastocysts after delayed insemination

Beatty

Coulter

1978

Genet. Res.

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The origin of triploid (3N) 5j-day blastocysts in rabbits is inferred from the segregation of sex chromosomes and of an autosomal M-marker whose properties are described. 39 triploids and no tetraploids were scored among 1454 chromosomally scored blastocysts. A delay of 8 h between an ovulatory injection and subsequent insemination raised the estimated normal incidence of 0-59 % triploid blastocysts to 3-13 %. The increase is ascribed primarily to digyny (17 blastocysts), and to diandry probably mediated by dispermy (1 blastocyst). The triploid components of the two 2N/3N mosaics and the one 3N/6N were digynic. Neither superovulation nor insemination of excessive numbers of spermatozoa could be shown to give rise to triploid embryos. The diandric triploid was XYY, the first of this constitution apparently reported in the rabbit. There was some evidence that XX Y triploid blastocysts up to 5^-day gestation are more viable than XXX. In the 2N/3N mosaics each component had been entered by one spermatozoon, and the diploid component could not have been merely a contamination by diploid maternal somatic cells. In 2N/4N, 2N/4N/8N and 3N/6N mosaics, each polyploid component showed an exact doubling of the marker chromosome constitution of a component of lower ploidy; their origin is ascribed to doubling or redoubling of chromosome number in isolated embryonic cells. With earlier data included, 49-08 (+ S.B. 1-96) % of 652 diploid blastocysts were 1 7 . 460 non-experimental weaned rabbits were all diploid.

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“…However, in agreement with Shaver & Carr (1967, 1969, 8 h delay gave a relatively high yield of triploids (3-13%), though not so high as reported by them (12-5% after 6-9 h delay).…”

Section: (Iii) Properties Of the M-markersupporting

confidence: 87%

Section: (V) the Relative Viability Of XXX And Xx Y Triploidsmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Digynic triploidy in rabbit blastocysts after delayed insemination

Beatty

Coulter

1978

Genet. Res.

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“…Hsu et al (1975) have concluded that although the most obvious secondary con strictions are equivalent to NORs which are in turn equivalent to sites of rDNA, second ary constrictions are not absolutely equated with NORs. In the rabbit, secondary con strictions have been reported on medium to small-sized metacentric chromosomes (Prunieras et al, 1965;Shaver, 1968) in addi tion to the subtelocentric and acrocentric chromosomes 13, 16, and 20 which stain with silver.…”

mentioning

confidence: 99%

Location of the 18S and 28S rRNA cistrons in the genome of the domestic rabbit (<i>Oryctolagus cuniculus</i> L.)

Pa¹

1980

Cytogenet Genome Res

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The genomic location of rDNA in the rabbit was determined by in situ hybridization with ¹²⁵I-labeled ribosomal RNA (rRNA). The results demonstrate the presence of rRNA genes on chromosomes 13, 16, and 20, all of which have secondary constrictions, and on the telomere of the long arm of 21. The number of genes, as assessed by grain counts, was not equally distributed among the chromosomes. Chromosome 20 had the most copies and along with 13 and 16 carried significantly more genes than 21.

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“…After size determina¬ tion, by a filar micrometer eyepiece, the blastocysts were separately placed in fresh culture medium to which colchicine (0-5 µg/ml) had been added. Following incubation for \\ hr at 37°C in 5% C02 in air, chromo¬ some preparations were made by a modification of the method of Shaver & Carr (1967 Nichols, Levan, Hansen-Melander & Melander (1965). The numbering of the autosomal pairs followed that of Shaver & Carr (1967).…”

mentioning

confidence: 99%