Chromosome Counts in a Polyploid Series of Saccharomyces

McClary, Dan O.; Williams, Marion A.; Lindegren, Carl C.; Ogur, Maurice

doi:10.1128/jb.73.3.360-364.1957

Cited by 24 publications

(6 citation statements)

References 20 publications

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“…HCL-CIE~SA'S : In hydrolyzed preparations stained with Giemsa's, the chromatin occupies the same portion of the nucleus as in Feulgen-acetocarmine preparations. We have confirmed the earlier observations of others and ourselves (29,38,44) that this portion of the nucleus becomes transformed into a cluster of chromosomes at the beginning of meiosis. Many nuclei in acid-Giemsa preparations are traversed by the same clear, narrow fissures which we have already encountered in the nuclei of Feulgen specimens.…”

Section: Observations On Living Cellssupporting

confidence: 92%

“…They are most strongly Feulgen positive at the time when the D N A content of the cells abruptly doubles (52,53,54), that is, in the compact "resting" nuclei of cells with small buds. There is no further increase in D N A content until the emergence of the next generation 148 T h E JOURNAL OF CELL BIOLOGY • VOLUME 29,1966 of buds. The pale Feulgen reaction of dividing nuclei ( Figs.…”

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confidence: 99%

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A Fiber Apparatus in the Nucleus of the Yeast Cell

Robinow

Marak

1966

The Journal of Cell Biology

338

119

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The structure and mode of division of the nucleus of budding yeast cells have been studied by phase-contrast microscopy during life and by ordinary microscopy after Helly fixation. The components of the nucleus were differentially stained by the Feulgen procedure, with Giemsa solution after hydrolysis, and with iron alum haematoxylin. New information was obtained in ceils fixed in Helly's by directly staining them with 0.005% acid fuchsin in 1% acetic acid in water. Electron micrographs have been made of sections of cells that were first fixed with 3% glutaraldehyde, then divested of their walls with snail juice, and postfixed with osmium tetroxide. Light and electron microscopy have given concordant information about the organization of the yeast nucleus. A peripheral segment of the nucleus is occupied by relatively dense matter (the ':peripheral duster" of Mundkur) which is Feulgen negative. The greater part of the nucleus is filled with fine-grained Feulgen-positive matter of low density in which chromosomes could not be identified. Chromosomes become visible in this region under the light microscope at meiosis. In the chromatin lies a short fiber with strong affinity for acid fuchsin. The nucleus divides by elongation and constriction, and during this process the fiber becomes long and thin. Electron microscopy has resolved it into a bundle of dark-edged 150 to 180 A filaments which extends between "centriolar plaques" that are attached to the nuclear envelope.

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Section: Observations On Living Cellssupporting

confidence: 92%

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confidence: 99%

A Fiber Apparatus in the Nucleus of the Yeast Cell

Robinow

Marak

1966

The Journal of Cell Biology

338

119

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“…This preparation had been squashed. mon with McClary et al (22) I have seen countable chromosomes in baker's yeast only at meiosis. This work will be described in a separate paper.…”

Section: Figure 57mentioning

confidence: 77%

MITOSIS IN THE YEAST LIPOMYCES LIPOFER

Robinow

1961

The Journal of Cell Biology

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A study of mitosis in Lipomyces has been carried out because preliminary observations by Ganesan and Roberts, 1959 (9), had indicated that the nucleus of this yeast might be unusually favourable for morphological observations. This impression has proved correct.The chromosomes of Lipomyces are visible as separate, countable bodies for the greater part of mitosis. The pattern of mitosis differs from the common one in that in Lipomyces the proper distribution of sister chromosomes is accomplished without the help of a spindle apparatus. At the end of prophase sister chromosomes are found in pairs which align themselves parallel to one another to form a palisade or stack whose long axis coincides with the axis of the impending division. At anaphase-telophase the stack of paired chromosomes fuses into a seemingly homogeneous cord which divides by constriction.

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“…To remove Ficoll and excess water during removal of nonlipid material (12), three washings with KCl were required instead of one. Total phospholipids in the extract were determined by assaying the phosphorus content of a portion (25 ,ul) of the lipid extract by the method of Chen et al (6), the phosphorus content being multiplied by 25 to give the phospholipid content. Sterols and sterol esters (after hydrolysis) were assayed as their acetates by gas-liquid chromatography with cholestane as an internal standard.…”

Section: Methodsmentioning

confidence: 99%

“…Cytochemical techniques revealed that yeast vacuoles concentrate cationic dyes such as neutral red (14) and contain lipid (31). The functions of these subcellular structures were for long the subject of intense dispute, not least because Lindegren and his colleagues (25) insisted that the yeast vacuole and nucleus were one and the same organelle. Sentandreu and Northcote (34) and Wiemken et al.…”

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Isolation and Properties of Two Classes of Low-Density Vesicles from Saccharomyces cerevisiae

et al. 1977

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A mixture of small (0.43-Am diameter) and large (0.62-,um diameter) lowdensity vesicles from spheroplasts of Saccharomyces cereuisiae was fractionated by rate centrifugation in a gradient of 0 to 8% (wt/vol) Ficoll to yield fractions rich (90 to 95%) in small or large vesicles. The large, but not small, vesicles swelled when diluted into mannitol solutions containing less than 0.4 M mannitol. The pH-electrophoretic mobility curve of the large vesicles showed that they are probably enclosed in a phospholipid-protein membrane. The dyes neutral red and toluidine blue, accumulated into large vesicles by intact cells and spheroplasts, were largely lost from large vesicles when these were separated from stained spheroplasts. Sudan black III stained small and large vesicles, both classes of vesicle retaining the stain on separation. Fractions rich in large vesicles contained proportionately more phospholipid and less free sterols, diacylglycerols, and free fatty acids compared with those enriched in small vesicles. The two classes of vesicles contained about the same proportions of esterified sterols and triacylglycerols. The free fatty acids in both small and large vesicles were free from unsaturated fatty-acyl residues; diacylglycerols and triacylglycerols contained appreciable proportions of unsaturated fatty-acyl residues. Small vesicles were richer in lipase activity, whereas the larger vesicles contained greater f3-glucanase and a-mannosidase activities. Phospholipase activity could not be detected in any of the fractions.

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Chromosome Counts in a Polyploid Series of Saccharomyces

Cited by 24 publications

References 20 publications

A Fiber Apparatus in the Nucleus of the Yeast Cell

A Fiber Apparatus in the Nucleus of the Yeast Cell

MITOSIS IN THE YEAST LIPOMYCES LIPOFER

Isolation and Properties of Two Classes of Low-Density Vesicles from Saccharomyces cerevisiae

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