Chronic heat stress increases insulin-like growth factor-1(IGF-1) but does not affect IGF-binding proteins in growing pigs

Xin, Hairui; Zhang, Xiaojun; Sun, Dengsheng; Zhang, Chuang; Hao, Ya; Gu, Xianhong

doi:10.1016/j.jtherbio.2018.08.008

Cited by 19 publications

(10 citation statements)

References 57 publications

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“…The rise of LA caused by glycolysis was also observed (Baumgard and Rhoads ). Moreover, the ascension of IGF‐1 level indicated the acceleration of glucose metabolism (Xin et al ). Stress can activate the hypothalamic–pituitary–adrenal (HPA) axis to release stress hormones such as ACTH, cortisol and adrenaline (Gou et al ; Yu et al ).…”

Section: Discussionmentioning

confidence: 99%

Effects of acute heat stress on intestinal microbiota in grow‐finishing pigs, and associations with feed intake and serum profile

Xiong

et al. 2019

J Appl Microbiol

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Aims This study was conducted to assess the effects of acute heat stress (HS) on intestinal microbiota, and the associations with the changes in feed intake (FI) and serum profile. Methods and Results Twenty four individually housed pigs (Duroc × Large White × Landrace, 30 ± 1 kg body weight) were randomly assigned to receive one of three treatments (8 pigs/treatment): (i) thermal neutral (TN) conditions (25 ± 1°C), (ii) HS conditions (35 ± 1°C), (iii) pair‐feeding (PF) with HS under TN conditions. After 24‐h treatment, pigs were monitored to assess FI, and samples of serum and faeces were collected to investigate serum profile, microbial composition and short chain fatty acids (SCFAs). The results showed that HS decreased (P < 0·05) FI compared with the TN group. Compared with TN group, HS changed the serum profile by affecting biochemical parameters and hormones related with energy metabolism and stress response; immune indicators were also altered in HS group. Most of changes in serum profile were independent of FI reduction. Additionally, HS shifted the diversity and composition of faecal microbial community by increasing (P < 0·05) Proteobacteria and decreasing (P < 0·05) Bacteroidetes. Moreover, HS decreased (P < 0·05) the concentrations of propionate, butyrate, valerate, iso‐valerate and total SCFAs in faeces in an FI‐independent manner. Furthermore, the Spearman correlation analysis implied that changes of serum profile have potential correlation with alterations of faecal microbiota and their SCFAs metabolites in acute HS‐treated grow‐finishing pigs. Conclusions Metabolism disorders caused by 24‐h acute HS associated with changes of faecal microbiota and their SCFAs metabolites in an FI‐independent manner in grow‐finishing pigs. Significance and Impact of the Study These results give us a new insight of the intestinal damage caused by acute HS and the underlying mechanisms.

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Section: Discussionmentioning

confidence: 99%

Effects of acute heat stress on intestinal microbiota in grow‐finishing pigs, and associations with feed intake and serum profile

Xiong

et al. 2019

J Appl Microbiol

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“…Thus, decreased cholesterol levels in the liver suggest that long-term heat stress reduced cholesterol uptake from extrahepatic tissues. Over prolonged heat exposure, decreased cholesterol and TCBAs levels in the liver might contribute to altering the lipid metabolism, due to the decrease of feed intake for an extended period and the increase of the insulin concentration [55] may be closely related to HS-adaptation mechanism. The adaptation condition was achieved in the body, owing to the alteration of the metabolism induced by long-term heat exposure, which might adopt an extended period of heat stress as an adaptive and compensatory capability.…”

Section: Discussionmentioning

confidence: 99%

Running Head: Heat Affects Cholesterol and Bile Acid Alterations in Cholesterol and Bile Acids Metabolism in Large White Pigs during Short-Term Heat Exposure

Wei

Wen

Meng

et al. 2020

Animals

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Heat stress influences lipid metabolism independently of nutrient intake. It is not well understood how cholesterol and bile acid (BA) metabolism are affected by heat stress. To investigate the alterations of cholesterol and bile acids when pigs are exposed to short term heat stress, 24 Large White pigs (63.2 ± 9.5 kg body weight, BW) were distributed into one of three environmental treatments: control conditions (CON, 23 °C with ad libitum intake; n = 8), heat stress conditions (HS, 33 °C with ad libitum intake; n = 8), or pair-fed conditions (PF, 23 °C with the same amount to the feed consumed by the HS; n = 8) for three days. Compared with CON pigs, HS pigs reduced the average daily feed intake and average daily gain by 55% and 124%, respectively, and significantly increased rectal temperatures by 0.9 °C and respiration rates more than three-fold. The serum total cholesterol (TC), low-density lipoprotein-cholesterol, and triglycerides (TG) increased (p < 0.05), while hepatic TC, TG, and mRNA of 3-hydroxy-3-methylglutaryl-CoA reductase were reduced on day 3. Furthermore, liver taurine-conjugated BAs (TCBAs), including taurolithocholic acid, taurochenodeoxycholic acid (TCDCA), tauroursodeoxycholic acid, taurohyodeoxycholic acid, and taurocholic acid were elevated in HS pigs compared to CON and PF pigs (p < 0.05), and the level of chenodeoxycholic acid was more significant in the PF group than in the CON and HS groups. The concentration of ursodeoxycholic acid in the serum was higher in HS pigs than CON and PF pigs (p < 0.05), and TCDCA was increased in HS pigs compared with PF pigs (p < 0.05). Altogether, short-term HS reduced hepatic cholesterol levels by decreasing cholesterol synthesis, promoting cholesterol to TCBAs conversion, and cholesterol release to serum in growing pigs. This independently reduced feed intake might serve as a mechanism to protect cells from damage during the early period.

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“…The thermocycler (T100TM Thermal Cycler, Bio-Rad, Hercules, CA, USA) was used to synthesize the complimentary DNA and a CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA, USA) was used for real-time quantitative PCR (qPCR). To obtain the mRNA abundance of target and housekeeping (β-actin) [ 58 ] genes, specific primers ( Supplementary Table S3 ) for following genes were obtained from previous studies or designed in-house: MUC1 [ 59 ], mucin 2 ( MUC2 ) [ 59 ], EGFR [ 60 ], insulin-like growth factor-1 receptor ( IGF-1R ) [ 61 ], caspase-9 [ 62 ], Ki-67 [ 63 ], PPARγ [ 64 ], IGFBP1 [ 65 ], IGF-1 [ 66 ], mTOR [ 67 ], ATF4 [ 68 ], CSNK2A1 [ 69 ], OCLN [ 70 ], ZO1 [ 70 ], PRKCA , and GCN2 . The qPCR program was followed by a melt curve program.…”

Section: Methodsmentioning

confidence: 99%

A Mixture of Valine and Isoleucine Restores the Growth of Protein-Restricted Pigs Likely through Improved Gut Development, Hepatic IGF-1 Pathway, and Plasma Metabolomic Profile

Habibi

Goodarzi

Shili

et al. 2022

IJMS

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Valine (Val) alone or in combination with isoleucine (Ile) improves the growth under severe protein restriction; however, the underlying mechanisms remain unknown. In this study, we assessed whether Val/Ile-induced growth in protein-restricted pigs is associated with changes in gut development, hepatic insulin-like growth factor 1 (IGF-1) production, and blood metabolomics. Forty piglets were assigned to five dietary groups: positive control (PC) with standard protein content; low protein (LP) with very low protein content; and LP supplemented with Val (LPV), Ile (LPI), and Val and Ile (LPVI). LPVI reversed the negative effects of VLP diets on growth and gut morphology. Both LPV and LPVI restored the reduced transcript of IGF-1 while decreasing the transcript of insulin-like growth factor binding protein 1 (IGFBP1) in the liver. LPV and LPVI recovered the reduced plasma Val, glycine, and leucine concentrations, which were positively correlated with improved gut morphology and the hepatic IGF-1 gene expression and negatively correlated with hepatic IGFBP1 mRNA abundance. In conclusion, supplementation with a combination of Val and Ile into the VLP diets restored the decreased growth performance of pigs fed with these diets likely through improved gut development, hepatic IGF-1 expression and bioavailability, and plasma metabolomics profile.

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Chronic heat stress increases insulin-like growth factor-1(IGF-1) but does not affect IGF-binding proteins in growing pigs

Cited by 19 publications

References 57 publications

Effects of acute heat stress on intestinal microbiota in grow‐finishing pigs, and associations with feed intake and serum profile

Effects of acute heat stress on intestinal microbiota in grow‐finishing pigs, and associations with feed intake and serum profile

Running Head: Heat Affects Cholesterol and Bile Acid Alterations in Cholesterol and Bile Acids Metabolism in Large White Pigs during Short-Term Heat Exposure

A Mixture of Valine and Isoleucine Restores the Growth of Protein-Restricted Pigs Likely through Improved Gut Development, Hepatic IGF-1 Pathway, and Plasma Metabolomic Profile

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