Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa

Farías, Jorge G.; Zepeda, Andrea B.; Castillo, Rodrigo; Figueroa, Elías; Ademoyero, Oludamilola T; Pulgar, Víctor M.

doi:10.1111/and.12876

Cited by 6 publications

(6 citation statements)

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“…Spermatozoa are cells that are particularly susceptible to oxidative stress given their limited content of antioxidants, and hypoxia exposure has been shown to produce DNA damage in sperm cells [10]. Chronic hypobaric hypoxia (CHH) attenuates 8-oxoguanine glycosylase (OGG1) and OGG1-2a in rat spermatozoa, which may contribute to DNA damage under hypoxia exposure [11]. Hypobaric hypoxia reduces the quality of sperm DNA, including fragmentation and unpacking, and its DNA stability is diminished in the mouse [12].…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes

et al. 2019

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Background To illuminate the mechanisms underlying the high-altitude tolerance of Tibetan pig spermatozoa, proteomes of spermatozoa from Tibetan pigs raised in high and low altitudes were compared using a tandem mass tag (TMT)-labeled quantitative proteomics approach. Results A total of 77 differentially expressed proteins (DEPs) were identified. Gene Ontology (GO) analysis revealed DEPs that were predominantly associated with the actin cytoskeleton, the tricarboxylic acid (TCA) cycle, and adenosine triphosphate (ATP) metabolism, and were from 12 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Three subnetworks were significantly enriched and 10 centric proteins were identified by protein-protein interaction (PPI) network analysis. Relative expression levels of the proteins (ATP5H, CYCS, MYH9 and FN1) were confirmed using Western blotting. Conclusions Our study is the first to use a tandem mass tag (TMT) approach to analyze Tibetan pig spermatozoa, and provides a foundation to understand the mechanisms underlying the reproductive adaptations of Tibetan pigs to high-altitude environments. Electronic supplementary material The online version of this article (10.1186/s12864-019-5873-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes

et al. 2019

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“…; Farias et al . ). Another technique in which progress has been made in detecting damage in specific genes or regions of the genome is reverse transcription polymerase chain reaction (RT‐PCR) (Cawthon ; Bennetts & Aitken ; Fernandez‐Gonzalez et al .…”

Section: Effects Of Cryopreservation On Dna Quality and Gene Expressionmentioning

confidence: 97%

“…Currently, damage to spermatic DNA derived from the effects of ROS is evaluated through classic techniques such as 8-oxodG and 8-oxodA detection. It can be quantified by flow cytometry with specific probes or with a Western blot of 8-Oxoguanine glycosylase (OGG1), allowing the repair expression of OGG1 enzymes in the spermatozoa to be identified and evaluated (Mart ınez-P aramo et al 2009;Shaliutina et al 2013;Farias et al 2018). Another technique in which progress has been made in detecting damage in specific genes or regions of the genome is reverse transcription polymerase chain reaction (RT-PCR) (Cawthon 2002;Bennetts & Aitken 2005;Fernandez-Gonzalez et al 2008;P erez-Cerezales et al 2009).…”

Section: Effects Of Cryopreservation On Dna Quality and Gene Expressionmentioning

confidence: 99%

Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development

Figueroa

Lee‐Estévez

Valdebenito

et al. 2018

Reviews in Aquaculture

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Sperm DNA quality and gene expression are crucial for early embryo development. Abnormal genomic processes can cause irreversible damage to totipotent cells, thereby altering the capacity for cell differentiation. Cryopreservation is a complex procedure that exposes cells to extreme conditions; it is therefore important to determine whether cryopreservation affects genomic stability. Despite this, few studies have focused on the effects of cryopreservation on the genomic stability of fish sperm. Thus, information is lacking to determine how the cryopreservation of fish sperm affects embryo quality and subsequently aquaculture. The aim of this review was to compile background information pertaining to the study of genomic stability in cryopreserved sperm. In this way, we hope to characterize more clearly the embryonic development of fish that are of biological and economic interest. We also discuss the potential use of antioxidants in cryopreservation to help preserve DNA integrity, gene expression and embryo quality. There is a current need to evaluate the use of potential biomarkers of genomic integrity to establish the effects of cryopreservation on the DNA quality of fish gametes and embryos.

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“…Looking at single base substitution (SBS) signatures, more hypoxic tumors experienced signatures with etiology of defective homologous recombination-based repair and DNA mismatch repair (MMR) ( Bhandari et al, 2020 ). In vitro investigations have shown that hypoxia could lead to genomic instability through its downregulation of DNA repair genes and their respective pathways of MMR ( Lu et al, 2014 ; Cowman et al, 2021 ), homologous recombination repair (HRR) ( Bindra et al, 2005 ; Fanale et al, 2013 ), base excision repair (BER) ( Chan et al, 2014 ; Farias et al, 2018 ) and Fanconi anemia ( Scanlon and Glazer, 2014 ) (reviewed in ( Luoto et al, 2013 ; Begg and Tavassoli, 2020 )). Hypoxia has additionally been implicated in the repression of DNA replication genes and increased replication stress through stalled replication forks and decreased new origin firing that is time and oxygen dependent ( Pires et al, 2010 ; Ng et al, 2018 ) (reviewed in ( Luoto et al, 2013 ; Begg and Tavassoli, 2020 )).…”

Section: Introductionmentioning

confidence: 99%

Chronic hypoxia is associated with transcriptomic reprogramming and increased genomic instability in cancer cells

Khouzam

Sharda²,

Rao³

et al. 2023

Front. Cell Dev. Biol.

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Hypoxia afflicts the microenvironment of solid tumors fueling malignancy. We investigated the impact of long hypoxia exposure on transcriptional remodeling, tumor mutational burden (TMB), and genomic instability of cancer cells that were grouped based on their inherent sensitivity or resistance to hypoxia. A hypoxia score was used as a metric to distinguish between the most hypoxia-sensitive (hypoxia high (HH)), and most resistant (hypoxia low (HL)) cancer cells. By applying whole exome sequencing and microarray analysis, we showed that the HH group was indeed more sensitive to hypoxia, having significantly higher TMB (p = 0.03) and copy number losses (p = 0.03), as well as a trend of higher transcriptional response. Globally cells adapted by decreasing expression of genes involved in metabolism, proliferation, and protein maturation, and increasing alternative splicing. They accumulated mutations, especially frameshift insertions, and harbored increased copy number alterations, indicating increased genomic instability. Cells showing highest TMB simultaneously experienced a significant downregulation of DNA replication and repair and chromosomal maintenance pathways. A sixteen-gene common response to chronic hypoxia was put forth, including genes regulating angiogenesis and proliferation. Our findings show that chronic hypoxia enables survival of tumor cells by metabolic reprogramming, modulating proliferation, and increasing genomic instability. They additionally highlight key adaptive pathways that can potentially be targeted to prevent cancer cells residing in chronically hypoxic tumor areas from thriving.

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Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa

Cited by 6 publications

References 8 publications

Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes

Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes

Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development

Chronic hypoxia is associated with transcriptomic reprogramming and increased genomic instability in cancer cells

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