Chronic nickel bioaccumulation and sub-cellular fractionation in two freshwater teleosts, the round goby and the rainbow trout, exposed simultaneously to waterborne and dietborne nickel

Leonard, Erin M.; Banerjee, Upasana; D'Silva, Joshua J.; Wood, Chris M.

doi:10.1016/j.aquatox.2014.04.028

Cited by 24 publications

(21 citation statements)

References 59 publications

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“…Furthermore, the proportion of debris (i.e., membranes, granules, and nuclei) recovered in the other fractions was not assessed in our work even though this fraction is a significant one with respect to mass and represents more than half of the metal accumulated in many studies (Wallace and Luoma ; Rainbow et al ; Leonard et al ). Moreover, Andersson () has already observed that DNA could accumulate in the cytosol following subcellular fractionation and our own PCR analysis (Supporting Information Fig.…”

Section: Discussionmentioning

confidence: 93%

“…For O. mykiss in particular, our results show that an increase in strength or time of homogenization does not enable to reach the threshold enzymatic selection criteria values for homogenization efficiency (Table ). Selection criteria values could only be met with a two‐step homogenization method which had already been recommended in others studies on D. magna (Lapointe and Couture ), Lymnaea stagnalis (Ng et al ), O. mykiss (Leonard et al ), and Chaoborus larvae (Rosabal et al ).…”

Section: Discussionmentioning

confidence: 99%

“…Fractions can be pooled to infer on metal subcellular toxicity. For example, the MSFs (Wallace et al ; Campana et al ) or the biologically active metals (Taylor and Maher ; Leonard et al ) typically include both heat‐denaturated proteins (HDP) and mitochondria. As a result, if the mitochondrial matrix is pooled with HDP and not with heat stable protein (HSP) after the heat treatment, metals in the mitochondrial matrix will likely still be measured in MSF and the overlap between this fraction and the cytosol will not affect the interpretation of subcellular toxicity.…”

Section: Discussionmentioning

confidence: 99%

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Enzymatic validation of species‐specific protocols for metal subcellular fractionation in freshwater animals

Cardon

Caron

Rosabal

et al. 2018

Limnology & Ocean Methods

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The use of fractionation protocols to determine metal subcellular distribution in aquatic organisms has gained much interest over the last 15 yr, however, accurate separations among the different components of cells are challenging. Subcellular fractions separated with such an approach are operationally defined and a potentially significant difference can exist between anticipated and resulting fractions. This study customizes and validates subcellular partitioning protocols, for three different freshwater organisms representing a diversity of challenges for subcellular fractionation: Daphnia magna, Chironomus riparius, and liver of Oncorhynchus mykiss. Several protocols involving different homogenization methods, centrifugation speeds, or conservation conditions were tested, and their efficiencies were assessed using enzymatic biomarker assays. Our work allowed us to identify critical steps to improve separations. First, for D. magna, a crustacean with a reinforced chitinous exoskeleton, the use of a strong homogenization method using a sonicator is necessary. Second, for both invertebrates, we observed the leaking of the mitochondrial matrix during cell fractionation, regardless of the homogenization strength and conservation conditions. Therefore, we propose that the mitochondria fraction should be referred to as the mitochondrial membrane fraction, and the cytosol fraction should be identified as the cytosol and mitochondrial matrix fraction. Third, the presence of a lipid-rich layer during O. mykiss liver fractionation may lead to an overlap between mitochondria and cytosol and must be considered in the protocol development. Finally, lysosomes should not be pooled with the microsomes fraction without prior validation. Overall, this study provides a benchmark for future methodological studies on similar taxa.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Enzymatic validation of species‐specific protocols for metal subcellular fractionation in freshwater animals

Cardon

Caron

Rosabal

et al. 2018

Limnology & Ocean Methods

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“…Heavy metal have a potential threat to organisms which attribute to high toxicity as well as aquatic organisms including farmed fish have the ability to accumulate these metals in tissues by directly from the ambient water or by ingestion of food and then become potentially toxic when this accumulation increases to a considerably high level (Tsai et al, 2013, Leonard et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural effects on gill tissues induced in red tilapia Oreochromis sp. by a waterborne lead exposure

Aldoghachi

Azirun

Yusoff

et al. 2016

Saudi Journal of Biological Sciences

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Experiments on hybrid red tilapia Oreochromis sp. were conducted to assess histopathological effects induced in gill tissues of 96 h exposure to waterborne lead (5.5 mg/L). These tissues were investigated by light and scanning electron microscopy. Results showed that structural design of gill tissues was noticeably disrupted. Major symptoms were changes of epithelial cells, fusion in adjacent secondary lamellae, hypertrophy and hyperplasia of chloride cells and coagulate necrosis in pavement cells with disappearance of its microridges. Electron microscopic X-ray microanalysis of fish gills exposed to sublethal lead revealed that lead accumulated on the surface of the gill lamella. This study confirmed that lead exposure incited a difference of histological impairment in fish, supporting environmental watch over aquatic systems when polluted by lead.

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“…Observa-se que o aumento destas enzimas nas brânquias e fígado, em relação aos níveis basais, decorre, em sua maioria, na exposição com compostos organoclorados (Simonato et al, 2010). (Giguère et al, 2006;Leonard et al, 2014 ). A metaloteoneína é uma proteína induzida pela exposição a metais e sua função é se ligar a estes elementos e assim, impedir os efeitos tóxicos que eles causariam.…”

Section: Análises Do Estresse Oxidativo: Hepático E Branquialunclassified

Efeito do alumínio e manganês, em pH ácido, nos parâmetros de estresse oxidativo em machos de Astyanax altiparanae (Characiformes: Characidae)

Abdalla¹

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À CAPES pela bolsa concedida para realização deste trabalho. À CESP de Paraibuna pela disponibilização dos animais para o estudo realizado neste trabalho. À Profa. Renata Guimarães Moreira Whitton pela orientação, compreensão, incentivo, paciência e confiança, acreditando no meu crescimento profissional e pessoal. Agradeço também pela grande amizade construída, que levarei por toda a minha vida.

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Chronic nickel bioaccumulation and sub-cellular fractionation in two freshwater teleosts, the round goby and the rainbow trout, exposed simultaneously to waterborne and dietborne nickel

Cited by 24 publications

References 59 publications

Enzymatic validation of species‐specific protocols for metal subcellular fractionation in freshwater animals

Enzymatic validation of species‐specific protocols for metal subcellular fractionation in freshwater animals

Ultrastructural effects on gill tissues induced in red tilapia Oreochromis sp. by a waterborne lead exposure

Efeito do alumínio e manganês, em pH ácido, nos parâmetros de estresse oxidativo em machos de Astyanax altiparanae (Characiformes: Characidae)

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