Chronic rhinosinusitis and biofilms

Ramadan, Hassan H.; Sanclement, Jose A.; Thomas, John G.

doi:10.1016/j.otohns.2004.11.011

Cited by 150 publications

(120 citation statements)

References 16 publications

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“…They defined the tower-shaped formations comprised of aggregates of matrix-encased bacteria as the morphological hallmark of biofilms. Ramadan et al [22] also reported evidence of biofilms in specimens obtained from all of five patients by SEM and transmission electron microscopy (TEM). The morphological criteria for biofilms by SEM are water channels, 3-D structure, and matrix-embedded spherical or elliptical bodies within the bacteria ranging from 0.05 to 5.0 lm.…”

Section: Discussionmentioning

confidence: 94%

Clinical Characteristics of Biofilms in Patients with Chronic Rhinosinusitis: A Prospective Case–Control Study

Jung

Eog

Kang

et al. 2014

Indian J Otolaryngol Head Neck Surg

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Recent studies have indicated that biofilms are involved in the pathogenesis of recurrent and recalcitrant chronic rhinosinusitis (CRS). The present study was performed to evaluate the presence of biofilms and to evaluate the relationships between the presence of biofilms and clinical features of CRS. A total of 33 patients were included in this study. Maxillary sinus mucosa from 26 CRS patients and the ethmoid mucosa from 7 patients undergoing septoplasty were collected. Biofilms were evaluated by scanning electron microscopy. Preoperative symptom scores, preoperative and intraoperative nasoendoscopic findings, and postoperative healing period were compared between the groups. Biofilms were detected in 14 (42.4 %) of the 33 patients. Biofilms were present in 13 (50 %) of the 26 patients in the CRS group, but in only one (14.3 %) of the seven patients in the control group. There were no significant differences in preoperative symptom scores or preoperative nasal Lund-Kennedy endoscopic scores between the groups. However, the average LundMackay and intraoperative sinus Lund-Kennedy endoscopic scores were significantly higher in biofilm-positive than biofilm-negative patients (P \ 0.05). In the follow-up period, the healing time was significantly longer in biofilmpositive than biofilm-negative patients (P \ 0.05). This study suggested that the presence of bacterial biofilms may contribute to the pathogenesis of CRS and the clinical characteristics of CRS patients after endoscopic sinus surgery.

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Section: Discussionmentioning

confidence: 94%

Clinical Characteristics of Biofilms in Patients with Chronic Rhinosinusitis: A Prospective Case–Control Study

Jung

Eog

Kang

et al. 2014

Indian J Otolaryngol Head Neck Surg

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“…Moreover, several studies describe biofilms consisting of unidentified bacteria on the surfaces and crypts of adenoids removed from children being treated for COM, CRS, chronic adenotonsillitis, and OSA and within the tissue and crypts of inflamed tonsils from children with chronic tonsillitis (1,11,38,55), demonstrating that uncharacterized bacterial biofilms are present in other chronic URT infections. Biofilm infections are clinically significant because these three-dimensional (3D), adherent, organized communities of bacteria are far more recalcitrant to antibiotic therapy and killing by host phagocytic cells (27,30,35).…”

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confidence: 99%

Adenoid Reservoir for Pathogenic Biofilm Bacteria

et al. 2011

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Biofilms of pathogenic bacteria are present on the middle ear mucosa of children with chronic otitis media (COM) and may contribute to the persistence of pathogens and the recalcitrance of COM to antibiotic treatment. Controlled studies indicate that adenoidectomy is effective in the treatment of COM, suggesting that the adenoids may act as a reservoir for COM pathogens. To investigate the bacterial community in the adenoid, samples were obtained from 35 children undergoing adenoidectomy for chronic OM or obstructive sleep apnea. We used a novel, culture-independent molecular diagnostic methodology, followed by confocal microscopy, to investigate the in situ distribution and organization of pathogens in the adenoids to determine whether pathogenic bacteria exhibited criteria characteristic of biofilms. The Ibis T5000 Universal Biosensor System was used to interrogate the extent of the microbial diversity within adenoid biopsy specimens. Using a suite of 16 broad-range bacterial primers, we demonstrated that adenoids from both diagnostic groups were colonized with polymicrobial biofilms. Haemophilus influenzae was present in more adenoids from the COM group (P ‫؍‬ 0.005), but there was no significant difference between the two patient groups for Streptococcus pneumoniae or Staphylococcus aureus. Fluorescence in situ hybridization, lectin binding, and the use of antibodies specific for host epithelial cells demonstrated that pathogens were aggregated, surrounded by a carbohydrate matrix, and localized on and within the epithelial cell surface, which is consistent with criteria for bacterial biofilms.

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“…In our case, the colonization was dominated by Mucor species, which do rarely form fungus balls (Table 1) and are mostly related to an acute fulminant invasive mycosis of the sinuses (Dhong 2000, Goodnight 1993, Henderson 1988, Pérez Fernández 2001, Ramadan 2006, and Saydam 1997 Dhong, Jung and Park (2000) refer to two cases of Mucor fungus balls but details could not be retrieved A fungus ball appears usually in one sinus, most frequently the maxillary, and is noninvasive. A rare case of an involvement of more than one sinus was presented by Chao (2004).…”

Section: Discussionmentioning

confidence: 72%

“…Mucor colonization of the sinuses is rare and usually occurs without predisposing factors in immunocompetent individuals in contrast to mucormycosis which is the second most frequent mycosis caused by moulds in immunocompromised patients (Sugar 1992). In these patients, the species are mostly related to an acute fulminant invasive mycosis of the sinuses (Dhong 2000, Henderson 1988, and Ramadan 2006. Gamba et al (1986) point out that paranasal sinus involvement can commence early in the course of the disease with radiological signs of mucosal thickening without formation of air-fluid level and rarely with bone erosion.…”

Section: Introductionmentioning

confidence: 99%

Erosive and Expansile Lesion of the Maxillary Sinus by a Sinus Fungus Ball Mimicking Malignancy

Dietrich¹,

Mitsimponas²,

Koloutsos³

et al. 2013

IJCRM

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Paranasal sinus fungus balls are a form of fungal infection, mostly associated with the maxillary sinus. They are noninvasive lesions that usually develop in immunocompetent individuals with no predisposing factors. Fungus balls are mostly related to Aspergillus species, whereas Mucor species have been rarely reported. Transformation of a fungus ball into an invasive mycosis may commence when the patient becomes immunosuppressed. It is of clinical importance to distinguish fungus balls from common sinusitis, neoplasia, hemorrhage, and other conditions. Histopathological investigation reveals the presence of nonseptate hyphae, whereas cultures often fail to detect Mucor species. Magnetic resonance imaging (MRI) is more sensitive than computed tomography (CT) scan in diagnosing fungal sinusitis, but there is no clear superiority of one imaging modality over the other. CT remains the method of choice. We present a case of a Mucor fungus ball of the maxillary sinus that transformed into an erosive noninvasive Mucor mycosis in patient with concomitant oral cancer. Diagnostic reasoning and difficulties in radiological diagnosis are being discussed.

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Chronic rhinosinusitis and biofilms

Abstract: This is the first documentation of biofilms in association with chronic rhinosinusitis. Further investigation is warranted, especially with control research subjects.

Cited by 150 publications

References 16 publications

Clinical Characteristics of Biofilms in Patients with Chronic Rhinosinusitis: A Prospective Case–Control Study

Clinical Characteristics of Biofilms in Patients with Chronic Rhinosinusitis: A Prospective Case–Control Study

Adenoid Reservoir for Pathogenic Biofilm Bacteria

Erosive and Expansile Lesion of the Maxillary Sinus by a Sinus Fungus Ball Mimicking Malignancy

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