Chronic social isolation exerts opposing sex-specific consequences on serotonin neuronal excitability and behaviour

Oliver, David K.; Intson, Katheron; Sargin, Derya; Power, Saige K; McNabb, Janice; Ramsey, Amy J.; Lambe, Evelyn K.

doi:10.1016/j.neuropharm.2020.108015

Cited by 28 publications

(26 citation statements)

References 54 publications

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“…The paucity of rodent studies in females is particularly unfortunate, since the prevalence of anxiety, depressive symptoms, eating disorders, and subclinical disordered eating behaviors is higher in women ( Jacobi et al, 2004 ; Hudson et al, 2007 ; Altemus et al, 2014 ). Moreover, single housing can exert opposite effects in males and females ( Oliver et al, 2020 ), which is rarely considered when using this manipulation to measure feeding or other behaviors.…”

Section: Introductionmentioning

confidence: 99%

Assessing the effects of stress on feeding behaviors in laboratory mice

François

Delgado

Shargorodsky

et al. 2022

eLife

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Stress often affects eating behaviors, increasing caloric intake in some individuals and decreasing it in others. The determinants of feeding responses to stress are unknown, in part because this issue is rarely studied in rodents. We focused our efforts on the novelty-suppressed feeding (NSF) assay, which uses latency to eat as readout of anxiety-like behavior, but rarely assesses feeding per se. We explored how key variables in experimental paradigms – estrous and diurnal cyclicity, age and duration of social isolation, prandial state, diet palatability, and elevated body weight – influence stress-induced anxiety-like behavior and food intake in male and female C57BL/6J mice. Latency to eat in the novel environment is increased in both sexes across most of the conditions tested, while effects on caloric intake are variable. In the common NSF assay (i.e., lean mice in the light cycle), sex-specific effects of the length of social isolation, and not estrous cyclicity, are the main source of variability. Under conditions that are more physiologically relevant for humans (i.e., overweight mice in the active phase), the novel stress now elicits robust hyperphagia in both sexes . This novel model of stress eating can be used to identify underlying neuroendocrine and neuronal substrates. Moreover, these studies can serve as a framework to integrate cross-disciplinary studies of anxiety and feeding related behaviors in rodents.

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Section: Introductionmentioning

confidence: 99%

Assessing the effects of stress on feeding behaviors in laboratory mice

François

Delgado

Shargorodsky

et al. 2022

eLife

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“…In rodent models, although stress is often linked to the consumption of "comfort foods" (90,91), this is not the case for all stressors. For example, in males, social isolation (92,93) and chronic mild stress (94-98) can decrease intake of sucrose.…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

“…Measurement of individual food intake in rodents usually necessitates single housing, which has sex-specific effects on the function of the hypothalamus-pituitary-adrenal axis (122-124) and feeding behaviors (93). In the studies analyzed here, 63% housed the animals individually, and only 7% compared it with group housing (Figure 4).…”

Section: Consideration Of Stressors That Are Incorporated Into Paradigms Involving Stress and Feedingmentioning

confidence: 99%

“…It is not possible to make general claims about the effect of "social stress" or "physical stress" on food intake, because stressors of the same class can have different effects on food intake in rodents. For example, in males, social isolation and overcrowding decrease feeding (92,93,125), while chronic social defeat stress promotes hyperphagia (111,126). In females, depriving access to maternal care can have opposite effects, depending on the severity of the J o u r n a l P r e -p r o o f manipulation.…”

Section: Type Of Stressmentioning

confidence: 99%

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A Framework for Developing Translationally Relevant Animal Models of Stress-Induced Changes in Eating Behavior

François¹,

Fernández-Gayol²,

Zeltser³

2022

Biological Psychiatry

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…Interestingly, despite the lack of CIE/WD-mediated effects on SK-sensitive mAHP amplitudes, our data indicate that the mAHP amplitude is larger in female BLA-NAC neurons compared to males, suggesting there may be sex differences in SK channels. Recent studies have demonstrated sex differences in how SK channels inhibitors alter neuronal excitability after chronic stress in other brain regions like the dorsal raphe (Oliver et al, 2020). Moreover, the mAHP amplitude is sex-dependent in the entorhinal cortex layer VI, leading to sex differences in neuronal excitability (Chung & Bailey, 2019).…”

Section: Bla-nac Projectionmentioning

confidence: 99%

Chronic Alcohol Dysregulates Glutamatergic Function in the Basolateral Amygdala in a Projection- and Sex-Specific Manner

Price

McCool

2021

Preprint

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Chronic intermittent ethanol (CIE) produces alcohol dependence, facilitates anxiety-like behavior, and increases post-CIE alcohol intake. The basolateral amygdala (BLA) is one of several brain regions that regulates anxiety-like behavior and alcohol intake through downstream projections. The BLA receives information from two distinct input pathways. Afferents from medial structures like the thalamus and prefrontal cortex enter the BLA through the stria terminalis whereas lateral cortical structures like the anterior insula cortex enter the BLA through the external capsule. CIE induces input- and sex-specific adaptations to glutamatergic function in the BLA. Previous studies sampled neurons throughout the BLA, but did not distinguish between projection-specific populations. The current study investigated BLA neurons that project to the NAC (BLA-NAC neurons) or the BNST (BLA-BNST neurons) as representative "reward" and "aversion" BLA neurons, and showed that CIE alters glutamatergic function and excitability in a projection- and sex-specific manner. CIE increases glutamate release from stria terminalis inputs only onto BLA-BNST neurons. At external capsule synapses, CIE increases postsynaptic glutamatergic function in male BLA-NAC neurons and female BLA-BNST neurons. Subsequent experiments demonstrated that CIE enhanced the excitability of male BLA-NAC neurons and BLA-BNST neurons in both sexes when glutamatergic but not GABAergic function was intact. Thus, CIE-mediated increased glutamatergic function facilitates hyperexcitability in male BLA-NAC neurons and BLA-BNST neurons of both sexes.

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Chronic social isolation exerts opposing sex-specific consequences on serotonin neuronal excitability and behaviour

Cited by 28 publications

References 54 publications

Assessing the effects of stress on feeding behaviors in laboratory mice

Assessing the effects of stress on feeding behaviors in laboratory mice

A Framework for Developing Translationally Relevant Animal Models of Stress-Induced Changes in Eating Behavior

Chronic Alcohol Dysregulates Glutamatergic Function in the Basolateral Amygdala in a Projection- and Sex-Specific Manner

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