2009
DOI: 10.1016/j.ejphar.2009.03.065
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Chronic treatment with recombinant human erythropoietin exerts renoprotective effects beyond hematopoiesis in streptozotocin-induced diabetic rat

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Cited by 46 publications
(44 citation statements)
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“…According to the unifying hypothesis, hyperglycaemia-induced mitochondrial overproduction of ROS induces PARP activation and excess methylglyoxal formation with subsequent substrate overflow due to polyribosylation-mediated inhibition of GAPDH [1,32]. Our study and that of others are consistent in demonstrating that glycaemia and early glycation products are not affected by suberythropoietic erythropoietin doses [36]. Thus, the reduction of AGE and glycoxidation product formation is unrelated.…”
Section: Discussionsupporting
confidence: 78%
See 1 more Smart Citation
“…According to the unifying hypothesis, hyperglycaemia-induced mitochondrial overproduction of ROS induces PARP activation and excess methylglyoxal formation with subsequent substrate overflow due to polyribosylation-mediated inhibition of GAPDH [1,32]. Our study and that of others are consistent in demonstrating that glycaemia and early glycation products are not affected by suberythropoietic erythropoietin doses [36]. Thus, the reduction of AGE and glycoxidation product formation is unrelated.…”
Section: Discussionsupporting
confidence: 78%
“…Although speculative, a link between both reduced methylglyoxal formation and matrix modification and AKT phosphorylation of retinal cells is conceivable. In the diabetic kidney, erythropoietin exerts antioxidant properties by inhibiting renal activation and overexpression of NADPH oxidase [36]. A similar mechanism may be operative in other diabetic tissues.…”
Section: Discussionmentioning
confidence: 99%
“…32 In brief, the total RNAs were isolated from rat kidney by the acid guanidium thiocyanate-phenol-chloroform method using Isogen reagent (Nippon Gene, Tokyo, Japan). First-strand cDNA was synthesized from total RNA (1 mg) with a commercially available RT kit (High Capacity cDNA Reverse Transcription Kit; Applied Biosystems, Foster City, CA, USA) using random primers.…”
Section: Rna Isolation and Reverse Transcription-polymerase Chain Reamentioning
confidence: 99%
“…32 In brief, total protein was extracted from kidneys using iced Tris buffer (5 mmol l À1 , pH 7.4) containing the protease inhibitors. The protein extracts were separated with sodium dodecyl sulfate-polyacrylamide gel electrophoresis, electrotransferred to membranes and blocked overnight with 4% non-fat dry milk.…”
Section: Protein Extraction and Western Blottingmentioning
confidence: 99%
“…17 The similar mechanism has also been reported in other kidney disease models, such as acute kidney injury induced by ischemia reperfusion injury 18 and lipopolysaccharide, 19 puromycin aminonucleosideinduced nephrotic syndrome, 20 and streptozotocininduced diabetic nephropathy. 21 The second possible mechanism is the inhibitory effect of EPO on oxidative stress injury. EPO has been reported to have direct antioxidative action by exploiting intracellular anti-oxidative mechanisms such as heme oxygenase-1 and glutathione peroxidase, as well as indirect anti-oxidative action by inducing iron depletion and thereby inhibiting irondependent oxidative injury.…”
Section: Discussionmentioning
confidence: 99%