Chronological characterization of sperm morpho-functional damage and recovery after testicular heat stress in Nellore bulls

Garcia‐Oliveros, Laura Nataly; Arruda, Rubens Paes de; Batissaco, Leonardo; Gonzaga, Vitor Hugo Guilger; Nogueira, Vinícius José Moreira; Florez-Rodriguez, S. A.; Almeida, Felipe P.; Alves, Maíra Bianchi Rodrigues; Pinto, Sâmara Cristine Costa; Nichi, Marcílio; Losano, João Diego de Agostini; Kawai, Giulia Kiyomi Vechiato; Celeghini, Eneiva Carla Carvalho

doi:10.1016/j.jtherbio.2022.103237

Cited by 8 publications

(5 citation statements)

References 53 publications

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“…On the 7th day after heat stress, we observed a decrease in the number of sperm in the epididymis of mice, and the corresponding transcriptional evidence was that a large number of genes down-regulated after heat stress were involved in meiosis and cell cycle processes. The study on bulls also found that the number of head-defective sperm increased and the mitochondrial membrane potential of sperm decreased within 7 days after heat stress [59]. The increase in apoptosis and the decrease in cell proliferation after heat stress are the main causes of testicular atrophy [23], and the decrease in sperm number may be related to the inhibition of spermatogonial stem cell proliferation [60].…”

Section: Discussionmentioning

confidence: 97%

Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders

Gan

Xie

et al. 2023

IJMS

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Spermatogenesis is temperature-dependent, and the increase in testicular temperature seriously affects mammalian spermatogenesis and semen quality. In this study, the testicular heat stress model of mice was made with a 43 °C water bath for 25 min, and the effects of heat stress on semen quality and spermatogenesis-related regulators were analyzed. On the 7th day after heat stress, testis weight shrank to 68.45% and sperm density dropped to 33.20%. High-throughput sequencing analysis showed that 98 microRNAs (miRNAs) and 369 mRNAs were down-regulated, while 77 miRNAs and 1424 mRNAs were up-regulated after heat stress. Through gene ontology (GO) analysis of differentially expressed genes and miRNA–mRNA co-expression networks, it was found that heat stress may be involved in the regulation of testicular atrophy and spermatogenesis disorders by affecting cell meiosis process and cell cycle. In addition, through functional enrichment analysis, co-expression regulatory network, correlation analysis and in vitro experiment, it was found that miR-143-3p may be a representative potential key regulatory factor affecting spermatogenesis under heat stress. In summary, our results enrich the understanding of miRNAs in testicular heat stress and provide a reference for the prevention and treatment of heat-stress-induced spermatogenesis disorders.

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Section: Discussionmentioning

confidence: 97%

Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders

Gan

Xie

et al. 2023

IJMS

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“…The significant increase in sperm abnormalities during heat stress is due to the impact of the stressor on the seminiferous epithelium, which disrupts the spermatogenesis process (Shahat et al, 2020;Garcia-Oliveros et al 2022). Sperm abnormalities in sheep due to heat stress can occur in various ways, such as insulation of the scrotum or a manipulated room temperature at 41°C in various exposure periods, depending on the severity of the temperature.…”

Section: Spermatozoa Qualitymentioning

confidence: 99%

Influence of Management Systems on Severity of Heat Stress and Reproductive Performance of Rams in the Tropics – A Review

Mazlishah,

Fauzi,

Nor

et al. 2024

Annals of Animal Science

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Sheep are an important source of protein for humans, and recent decades have seen a significant increase in the production of sheep-based products, thus indicating an increase in demand for this commodity. At a time when this sector is growing, sheep are exposed to various challenges. These include climate change, which can cause heat stress in sheep, including rams, which later negatively affects their growth and reproductive performance. Exposure to heat stress results in physiological and reproductive behaviour responses in rams, which could also be used to visually identify the effect of heat stress as it will affect the productivity of livestock. In addition, the year-round hot and humid climate of the tropics is expected to contribute to a higher probability of heat stress occurrences in rams reared in developing countries around the equator. Nevertheless, the adaptability of rams to heat stress varies according to their origin, whereby native breeds tend to adapt better. Despite that, native breeds still can be affected by adverse environmental conditions and rely on thermoregulation mechanisms to mitigate heat stress. This results in numerous negative physiological changes, such as sweating and increased heart rate. This review highlights the effects of heat stress on the physiology, reproductive behaviour and reproductive performance of male sheep. The review also discusses the impact of management systems on heat stress in rams, which affects sheep productivity.

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“…The ensuing hypoxia leads to increased reactive oxygen species production, which when in excess have a negative effect on spermatogenesis. 17 Differences between breeds, individual animals, and the intensity and duration of the heat insult influence the effect on spermatogenesis and whether the disruption in testicular function progresses to testicular degeneration. Scrotal insulation for 8 days was associated with Sertoli and Leydig cell dysfunction.…”

Section: Pathophysiologymentioning

confidence: 99%

Pathophysiology, diagnosis, and management of testicular degeneration in the bull

Koziol

Palmer²

2023

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Testicular degeneration in the bull is described as diffuse disintegration of seminiferous tubule structure and function causing a profound reduction in sperm concentration, scrotal circumference, and an increase in sperm morphologic abnormalities resulting in subfertility or permanent infertility. Degenerative changes within the seminiferous epithelium occur most commonly after an insult to spermatogenesis or may be idiopathic. Common insults include trauma, stress (environmental, heat, nutritional), or toxins. Idiopathic is most often associated with age-related changes. Clinical signs of testicular degeneration are discussed along with approaches to treatment and potential prognoses as related to treatment and management practices that can be employed by the general practitioner and specialist alike. This review will also encompass a discussion on the pathophysiology of this disease so that the practitioner has a detailed understanding of the disease, which improves their ability to employ evidence-based medicine and sound clinical reasoning.

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Chronological characterization of sperm morpho-functional damage and recovery after testicular heat stress in Nellore bulls

Cited by 8 publications

References 53 publications

Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders

Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders

Influence of Management Systems on Severity of Heat Stress and Reproductive Performance of Rams in the Tropics – A Review

Pathophysiology, diagnosis, and management of testicular degeneration in the bull

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