Chrysin alleviates testicular dysfunction in adjuvant arthritic rats via suppression of inflammation and apoptosis: Comparison with celecoxib

Darwish, Hebatallah A.; Arab, Hany H.; Abdelsalam, Rania M.

doi:10.1016/j.taap.2014.05.018

Cited by 48 publications

(39 citation statements)

References 47 publications

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“…A routine histopathology protocol was adopted as previously described [32]. In brief, air pouch lining specimens were harvested and fixed in 10% formalin for 24 h. Five micron paraffin embedded sections were stained with hematoxylin and eosin (H&E) and the slides were inspected under the light microscope (Leica Microsystems, Germany) by an observer unaware of sample identity.…”

Section: Methodsmentioning

confidence: 99%

“…Nitric oxide (NO) levels were estimated based on the method of Miranda et al [38] with the modification of replacing zinc sulfate instead of ethanol for protein precipitation [32]. The anti-oxidant defenses; reduced glutathione (GSH) and total anti-oxidant capacity (TAC) were determined according to the colorimetric method of Beutler et al [39] and the specific TAC kit (Cayman, USA), respectively.…”

Section: Methodsmentioning

confidence: 99%

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Camel Milk Attenuates Rheumatoid Arthritis Via Inhibition of Mitogen Activated Protein Kinase Pathway

Arab

Salama²,

Abdelghany

et al. 2017

Cell Physiol Biochem

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Background/Aims: Camel milk (CM) has shown beneficial anti-inflammatory actions in several experimental and clinical settings. So far, its effect on rheumatoid arthritis (RA) has not been previously explored. Thus, the current work aimed to evaluate the effects of CM in Adjuvant-induced arthritis and air pouch edema models in rats, which mimic human RA. Methods: CM was administered at 10 ml/kg orally for 3 weeks starting on the day of Freund’s adjuvant paw inoculation. The levels of TNF-α and IL-10 were measured by ELISA while the protein expression of NF-κBp65, COX-2 and iNOS was detected by immunohistochemistry. The expression of MAPK target proteins was assessed by Western blotting. Results: CM attenuated paw edema, arthritic index and gait score along with dorsal pouch inflammatory cell migration. CM lowered the TNF-α and augmented the anti-inflammatory IL-10 levels in sera and exudates of arthritic rats. It also attenuated the expression of activated NF-κBp65, COX-2 and iNOS in the lining of the dorsal pouch. Notably, CM inhibited the MAPK pathway signal transduction via lowering the phosphorylation of p38 MAPK, ERK1/2 and JNK1/2 in rat hind paws. Additionally, CM administration lowered the lipid peroxide and nitric oxide levels and boosted glutathione and total anti-oxidant capacity in sera and exudates of animals. Conclusion: The observed CM downregulation of the arthritic process may support the interest of CM consumption as an adjunct approach for the management of RA.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Camel Milk Attenuates Rheumatoid Arthritis Via Inhibition of Mitogen Activated Protein Kinase Pathway

Arab

Salama²,

Abdelghany

et al. 2017

Cell Physiol Biochem

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“…This pattern differs somewhat from that reported by Darwish et al . () who showed intense morphological alteration and tissue damage with degeneration of seminiferous tubules in testes of adjuvant arthritic rats after 20 days of induction. These findings suggest that the effects of AIA on the testis vary according to the period of evolution of the disease.…”

Section: Discussionmentioning

confidence: 87%

“…The present study could be complemented by the evaluation of FSH and LH levels. However, previous studies have shown that the concentrations of both hormones are higher in animals submitted to AIA (Clemens & Bruot, ; Darwish et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Adjuvant‐induced arthritis affects testes and ventral prostate of Wistar rats

et al. 2019

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Background Rheumatoid arthritis (RA) may reduce the testosterone production, thereby leading to testicular dysfunction and subfertility. Objectives This study aimed to evaluate whether adjuvant‐induced arthritis (AIA) induces histopathological and morphometric‐stereological alterations on testes with repercussions on the prostate, and alternatively, verifying AIA‐induced direct effects on the prostate, regardless of the testicular involvement. Material and methods Adult male Wistar rats were sham‐orchiectomized or orchiectomized. Twenty days after the surgery, these animals were injected with vehicle (SHAM and ORQ groups, respectively) or adjuvant (Mycobacterium tuberculosis) to induce arthritis (AIA and ORQ/AIA groups, respectively). Forty days later, testes and ventral prostate were processed for histopathological and morphometric‐stereological analyses, as well as to PCNA immunohistochemistry. Collagen deposit was evaluated in prostate. Circulating testosterone levels were determined 15 days post‐AIA induction in SHAM and AIA rats and 40th day in all groups. Results In the testes, AIA promoted histopathological changes characterized by an increase in the percentage of abnormal tubules and reduction in the height of the seminiferous epithelium, daily production of spermatozoa, and cellular proliferation. In the prostate, AIA decreased the luminal volume of the secretory ducts. In condition of androgenic deprivation due to the orchiectomy, AIA induced proliferation of the prostatic epithelium. Discussion The effects of arthritis on testes and prostate were observed 40 days post‐AIA induction, possibly results of the hypoandrogenism were already established on 15th day post‐induction, which is related to the decline of the steroidogenesis in the Leydig cells. On the other hand, the joint inflammatory process may also have direct repercussions upon the prostate, regardless of this hypoandrogenism. Conclusion AIA effects on reproductive tissues may be related to both hypoandrogenism and other direct inflammatory mechanisms. Possibly, these AIA effects on the testes and prostate occur at a stage in which the inflammatory process is most active, about 15–20 days after induction, remaining evident until the 40th day.

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“…Chrysin increase sperm concentration, boost testosterone and attenuate oxidative stress and apoptosis [9]. Propolis was found significantly increase testosterone level, body weight, the relative weight of epididymis, semen characteristics and seminal plasma enzymes and decreased the levels of free radicals and lactate dehydrogenase [10].…”

Section: Introductionmentioning

confidence: 91%

Effect of Propolis on Spermatogenic Cells Number and Diameter of Seminiferous Tubules in Male Mice (Mus musculus)

Nurkarimah

Hestianah

Wahjuni

et al. 2017

KLS

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The present research aimed to determine the effect of propolis in spermatogenic cells number and seminiferous tubules diameter. Group P0 served as control group, P1 (1,6 mg/0,5ml/day), P2 (3,2 mg/0,5 ml/day), P3 (6,4 mg/0,5 ml/day), and P4 (12,8 mg/0,5 ml/day) were given propolis ethanolic extract treatment orally and killed after 14 days. Spermatogenic cells number (spermatogonial cells, primary spermatocytes, spermatids) and seminiferous tubules diameter were observed. The result showed a lower number of spermatogonial in P1 and P2 groups, primary spermatocytes reduction in P2 group, spermatids increased in P1, P3, and P4 group. Seminiferous tubules diameter decreased in P2 and P3 group. P2 group (3,2 mg/0,5 ml/day) showed the lowest result in all parameters (p<0.05). However, oral administration of propolis at these dose for 14 days may decrease spermatogonial cells and primary spermatocytes and increase spermatids number in adult mice.

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Chrysin alleviates testicular dysfunction in adjuvant arthritic rats via suppression of inflammation and apoptosis: Comparison with celecoxib

Cited by 48 publications

References 47 publications

Camel Milk Attenuates Rheumatoid Arthritis Via Inhibition of Mitogen Activated Protein Kinase Pathway

Camel Milk Attenuates Rheumatoid Arthritis Via Inhibition of Mitogen Activated Protein Kinase Pathway

Adjuvant‐induced arthritis affects testes and ventral prostate of Wistar rats

Effect of Propolis on Spermatogenic Cells Number and Diameter of Seminiferous Tubules in Male Mice (Mus musculus)

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