Chrysin Effect in Prevention of Acetaminophen-Induced Hepatotoxicity in Rat

Abstract

Acetaminophen is a commonly used analgesic drug that induces hepatotoxicity at high doses and produces the acetaminophen metabolite N-acetyl-p-benzoquinone imine (NAPQI) through oxidase isoenzyme system. The antioxidant and anti-inflammatory activity of flavonoid chrysin has been reported in different studies. The present study was conducted to investigate the protective effect of chrysin on acute acetaminophen-induced hepatotoxicity. The cytotoxicity of chrysin on fibroblast cells was evaluated using MTT assay, and then, 54 rats were divided into nine groups of six, and acetaminophen (1500 mg/kg) was administered in all groups except for the control group, second and the seventh groups (40 mg/kg), and all groups were treated with chrysin for 14 days. Liver enzymes, inflammatory factors TNF-α and IL-2, and total antioxidant activity were measured in serum while liver tissue was histopathologically examined. Based on the MTT assay results, 31.25, 62.5, 125, 250, and 500 μg/mL chrysin had no adverse effects on healthy fibroblast cells (P < 0.05). Chrysin decreased the level of liver enzymes (ALT, AST, and ALP), which were previously increased after the use of acetaminophen (p < 0.05). The hepatoprotective effect and total antioxidant capacity increased in a dose-dependent manner and the effect of the highest concentration of chrysin was equal to the effect of silymarin (P < 0.05). TNF-α in groups 4 to 6 decreased in a dose-dependent manner (P = 0.04), and chrysin did not show any significant reducing effect on IL-2 compared to silymarin. Chrysin prevents the necrosis and injury of acute acetaminophen-induced hepatotoxicity by decreasing liver enzymes and TNF-α and increasing total antioxidant capacity and protecting the liver tissue.

Broad-Spectrum Preclinical Antitumor Activity of Chrysin: Current Trends and Future Perspectives

“…In this regard, chrysin reduces levels of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2). The ameliorative effect of chrysin on acetaminophen-mediated hepatotoxicity seems to be dose-dependent with more therapeutic effects at higher concentrations [ 72 ]. In addition to hepatoprotective activity, chrysin has shown potential neuroprotective effects.…”

Effects of Solanum lycopersicum L. (tomato) against isoniazid and rifampicin induced hepatotoxicity in wistar albino rats

“…This indicated that INH+RIF have induced toxicity in rat of group II, which is confirmed by the rise in AST, ALT and ALP levels. Elevated levels of these three enzymes showed abnormal integrity of liver cells (Mohammadi et al, 2019), which was resulted in the intracellular enzymes released into the systemic circulation.…”

Ameliorative effect of Alpinia officinarum Hance extract on nonylphenol‐induced reproductive toxicity in male rats

“…The human skin fibroblast cell line (HNFF‐P18) was purchased from the National Cell Bank of Iran (NCBI, Pasteur Institute). The method used in this study for MTT assay was previously described (Mohammadi et al., 2019). Briefly, fibroblast samples were cultured in special 75 cc flasks (SPL) and then used after the fifth passage.…”