CIAPIN1 inhibits the growth and proliferation of clear cell renal cell carcinoma

He, Lijie; Wang, Hanmin; Jin, Haifeng; Guo, Changcun; Xie, Hui; Yan, Kun; Li, Xiaohuai; Shen, Qing; Qiao, Tiankui; Chen, Guanglei; Chai, Na; Zhao, Lina; Dong, Quan; Zheng, Yan; Liu, Jie; Fan, Daiming

doi:10.1016/j.canlet.2008.10.044

Cited by 34 publications

(34 citation statements)

References 21 publications

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“…After incubation with MTT assay (Sigma, St. Louis, MO Q16 ), the cells were lysed in 150 mL of 100% dimethyl sulfoxide and the absorbance was tested at 490 nm with a 96-well plate reader (Dynex Technologies Q17 ). 24 Each experiment was performed in triplicate.…”

Section: Cell Proliferation Assay (Mtt Assay)mentioning

confidence: 99%

“…24 The transfected cells were harvested when they were 70% to 80% confluent. After washing in ice-cold phosphate-buffered saline, the cells were suspended in approximately 0.5 mL of 70% alcohol and kept at 4 C overnight.…”

Section: Flow Cytometrymentioning

confidence: 99%

“…The staining was performed as described previously. 24 Immunofluorescence and Immunohistochemistry Cultured cells were grown on 0.8 Â 0.8-cm slides, rinsed with ice-cold phosphate-buffered saline, and fixed in 4% paraformaldehyde. Immunofluorescence or immunohistochemistry staining was performed on cultured cells or paraffin-embedded sections, as described previously.…”

Section: Western Blot Analysismentioning

confidence: 99%

“…Forty-eight hours later, supernatants from four plates were pooled, filtered, and stored at 4 C shortterm. Lentivirus titers were determined by transfecting human embryonic kidney 293 T cells with a dilution series of the viral suspension, and lentivirus samples with a titer of 4 Â 10 8 transfection units/mL were stored at À80 C. 23,24 Peritoneal Exposure Model in Rats A chronic infusion model of nonuremic animal PD was used, as described previously. 6 …”

Section: Ethics Statementmentioning

confidence: 99%

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Twist Contributes to Proliferation and Epithelial-to-Mesenchymal Transition–Induced Fibrosis by Regulating YB-1 in Human Peritoneal Mesothelial Cells

Che

et al. 2015

The American Journal of Pathology

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Twist is overexpressed in high glucose (HG) damage of human peritoneal mesothelial cells (HPMCs) in vitro. Herein, we further identified its precise function related to fibrosis of peritoneal membranes (PMs). The overexpression and activation of Twist and YB-1 (official name, YBX1) and a transformed fibroblastic phenotype of HPMCs were found to be positively related to epithelial-mesenchymal transition progress and PM fibrosis ex vivo in 93 patients who underwent continuous ambulatory peritoneal dialysis (PD), and also in HG-induced immortal HPMCs and an animal model of PD. Evidence from chromatin immunoprecipitation and luciferase reporter assays supported that YBX1 is transcriptionally regulated by the direct binding of Twist to E-box. Overexpression of Twist and YB-1 led to an increase in epithelial-mesenchymal transition, proliferation, and cell cycle progress of HPMCs, which might contribute to PM fibrosis. In contrast, the silencing of Twist or YB-1 inhibited HG-induced growth and cell cycle progression of HPMCs; this led to a down-regulation in the expression of cyclin Ds and cyclin-dependent kinases, finally inhibiting PM fibrosis. Twist contributes to PM fibrosis during PD treatment, mainly through regulation of YB-1.

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Section: Cell Proliferation Assay (Mtt Assay)mentioning

confidence: 99%

Section: Flow Cytometrymentioning

confidence: 99%

Section: Western Blot Analysismentioning

confidence: 99%

Section: Ethics Statementmentioning

confidence: 99%

See 2 more Smart Citations

Twist Contributes to Proliferation and Epithelial-to-Mesenchymal Transition–Induced Fibrosis by Regulating YB-1 in Human Peritoneal Mesothelial Cells

Che

et al. 2015

The American Journal of Pathology

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“…CIAPIN1 is widely distributed in healthy fetal and adult tissues, and the gene is expressed in differentiated tissue and metabolically active tissue (4). However, its expression has been shown to be inhibited in certain types of cancerous tissues, including gastric cancer and clear cell renal cell carcinoma (5,6). These findings suggest that the expression of the CIAPIN1 gene may be associated with the suppression of tumor development.…”

Section: Introductionmentioning

confidence: 74%

Cytokine-induced apoptosis inhibitor 1 inhibits the growth and proliferation of multiple myeloma

Wang

Pan

2015

Molecular Medicine Reports

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Abstract. The present study investigated the differential expression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) in human multiple myeloma (MM) bone marrow tissue and adjacent healthy bone marrow tissue. In addition, the effect of a transduced CIAPIN1 gene on the growth of the RPMI-8226 human MM cell line was investigated. CIAPIN1 protein expression was detected in 32 samples of paraffin-embedded MM and adjacent healthy bone marrow tissue using immunohistochemistry. The CIAPIN1 gene (Ad-CIAPIN1, small interfering RNA) was inserted into a lentiviral vector and transfected into the RPMI-8226 human MM cell line. The expression of target proteins CIAPIN1 and insulin-like growth factor 1U (IGF-1), cell cycle-regulatory proteins and functional proteins was detected using western blotting. MTT and soft agar colony formation assays were conducted, and cellular tumorigenicity in nude mice was assessed, in order to investigate the proliferative capacity of cells in vitro and in vivo.Flow cytometry was applied in order to analyze changes in the cell cycle and cell apoptosis. CIAPIN1 expression was significantly reduced in cells from the 32 MM samples compared with those from healthy bone marrow (P<0.05). Upregulation of CIAPIN1, following transduction by lentiviral vectors, caused cells to arrest in G1/S phase of the cell cycle and significantly inhibited the growth of the RPMI-8226 MM cell line in vitro and in vivo. CIAPIN1 was shown to inhibit cell growth. Specifically, it inhibited cyclin-dependent kinase 2, cyclin-dependent kinase 4 and insulin-like growth factor-1. Increased expression of CIAPIN1 also led to an increase in the levels of p27 and Rb, an effect that may have been achieved via regulation of cell cycle proteins and functional proteins. The results of the present study suggest that downregulation of the CIAPIN1 gene in MM cells may be associated with the development of this disease. CIAPIN1 transfection in RPMI-8226 cells significantly inhibited the growth of tumor cells, suggesting that the CIAPIN1 gene is a potential tumor suppressor.

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Drosophila CIAPIN1 homologue is required for follicle cell proliferation and survival

Marzuk

Peretz

Bakhrat

2013

Developmental Dynamics

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CIAPIN1 inhibits the growth and proliferation of clear cell renal cell carcinoma

Cited by 34 publications

References 21 publications

Twist Contributes to Proliferation and Epithelial-to-Mesenchymal Transition–Induced Fibrosis by Regulating YB-1 in Human Peritoneal Mesothelial Cells

Twist Contributes to Proliferation and Epithelial-to-Mesenchymal Transition–Induced Fibrosis by Regulating YB-1 in Human Peritoneal Mesothelial Cells

Cytokine-induced apoptosis inhibitor 1 inhibits the growth and proliferation of multiple myeloma

Drosophila CIAPIN1 homologue is required for follicle cell proliferation and survival

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