Ciliated epithelial-specific and regional-specific expression and regulation of the estrogen receptor-β2 in the fallopian tubes of immature rats: a possible mechanism for estrogen-mediated transport process in vivo

Shao, Ruijin; Weijdegård, Birgitta; Fernández-Rodrı́guez, Julia; Egecioglu, Emil; Zhu, Changlian; Andersson, Niklas; Thurin‐Kjellberg, Ann; Bergh, Christina; Billig, Håkan

doi:10.1152/ajpendo.00101.2007

Cited by 50 publications

(45 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This may be the case in the oviduct, where ERb is also expressed in epithelial cell cilia. Although the function of this ER in the oviduct relative to CBF is not defined, it has recently been shown that, in the lung, ERb mediates a rapid, nongenomic effect to speed CBF (47,48). In our studies, we did not find that E2 alone directly affects CBF when assayed at 24 hours; however, E2 blocked P4-mediated CBF inhibition at that time, emphasizing the importance of hormone balance.…”

Section: Discussioncontrasting

confidence: 74%

Sex Hormone–Dependent Regulation of Cilia Beat Frequency in Airway Epithelium

Jain

Ray

Pan

et al. 2012

Am J Respir Cell Mol Biol

View full text Add to dashboard Cite

Previous studies have demonstrated a female disadvantage in airway diseases, such as asthma and bronchiectasis. The basis for this sex disparity is unknown. We hypothesized that the female sex hormone, progesterone (P4), inhibits functions of the normal airway mucociliary apparatus. P4 receptor (PR) expression was evaluated in human lung and cultured primary human airway epithelial cells isolated from male and female lung transplant donors. PR expression was restricted to the proximal region of the cilia of airway epithelia, and was similar in men and women. Expression of isoform PR-B was more abundant than PR-A in cells from both sexes. Airway epithelial cell exposure to P4 decreased cilia beat frequency (CBF) by 42.3% (67.2). Inhibition of CBF was prevented by coadministration of P4 with the active form of estrogen, 17b-estradiol, or the PR antagonist, mifepristone. P4 inhibition was time and dose dependent, with a significant decrease by 8 hours and maximal effect at 24 hours, accompanied by translocation of PR from the cilia to the nucleus. Inhibition of cilia beat was also prevented by treatment of cells with actinomycin D, suggesting that CBF inhibition is a transcriptionally mediated event. Together, these findings indicate that sex hormones influence the function of a key component of the mucociliary apparatus. These mechanisms may contribute to the sex disparity present in airway diseases and provide therapeutic targets for the treatment of these debilitating airway diseases.Keywords: cilia; progesterone receptor; cilia beat frequency; sex disparity; lung diseaseMultiple epidemiologic studies have established that women have more severe airway disease than men (1, 2). Women with asthma and chronic obstructive lung disease have more frequent and severe exacerbations than men, and a lower disease-specific quality of life (1, 3). Bronchiectasis affects women more commonly, and is more aggressive than in men (2, 4). For example, in cystic fibrosis (CF), a disease in which the impact of bronchiectasis has been extensively studied, women have worse outcomes and a decreased life expectancy compared with men, despite a similar cause of disease. This has been attributed specifically to a greater severity of lung disease, and is present even after adjusting for potentially confounding morphometric, nutritional, compliance, and microbial factors (4, 5). In addition, in models of Pseudomonas aeruginosa infection, one of the most common pathogens found in bronchiectasis, female mice are more affected than males, again for unclear reasons (6). Defects in mucociliary clearance are a central component to the development of bronchiectasis. We, therefore, hypothesize that female sex hormones regulate the mucociliary apparatus.The major female sex hormones, estrogen and progesterone (P4), vary in levels during ovulatory cycles, pregnancy, menopause, and with exogenous delivery. Although their roles in the uterus, ovary, oviduct, and breast have been well studied, expression and function in other tissues are less well desc...

show abstract

Section: Discussioncontrasting

confidence: 74%

Sex Hormone–Dependent Regulation of Cilia Beat Frequency in Airway Epithelium

Jain

Ray

Pan

et al. 2012

Am J Respir Cell Mol Biol

View full text Add to dashboard Cite

show abstract

“…Furthermore, truncated forms of both ER␣ and ER␤ have been identified in many estrogen target tissues and cell types (2,3,32,42), suggesting that the tissue/cell-specific responses to estrogen resulting in the divergence of biological function may at least in part be due to differences in the differential expression and regulation of ER␣/␤ isoforms. Recently, we have demonstrated the presence of ER␤ isoforms specifically in the ciliated epithelial cells of rat fallopian tube (56). Although the molecular mechanisms of ER␤ function in the fallopian tube still need to be clearly elucidated, our data suggest that ER␤ is involved in the estrogen-mediated regulation of calcium-dependent ciliated beating (56).…”

mentioning

confidence: 68%

“…Recently, we have demonstrated the presence of ER␤ isoforms specifically in the ciliated epithelial cells of rat fallopian tube (56). Although the molecular mechanisms of ER␤ function in the fallopian tube still need to be clearly elucidated, our data suggest that ER␤ is involved in the estrogen-mediated regulation of calcium-dependent ciliated beating (56). In contrast to ER␤, it has been postulated that ER␣ gene common polymorphisms in humans have been associated with female infertility (7), and ER␣ serves as a dominant regulator in fallopian tube development (44).…”

mentioning

confidence: 99%

Dynamic regulation of estrogen receptor-α isoform expression in the mouse fallopian tube: mechanistic insight into estrogen-dependent production and secretion of insulin-like growth factors

Shao

Egecioglu²,

Weijdegård³

et al. 2007

American Journal of Physiology-Endocrinology and Metabolism

Self Cite

View full text Add to dashboard Cite

Estrogen receptors (ERs) are members of the nuclear receptor superfamily and are involved in regulation of fallopian tube functions (i.e., enhancement of protein secretion, formation of tubal fluid, and regulation of gamete transport). However, the ER subtype-mediated mechanisms underlying these processes have not been completely clarified. Recently, we identified ER␤ expression and localization in rat fallopian tubes, suggesting a potential biological function of ER␤ related to calcium-dependent ciliated beating. Here we provide for the first time insight into the less studied ER␣ isoforms, which mediate estrogen-dependent production and secretion of IGFs in vivo. First, Western blot studies revealed that three ER␣ isoforms were expressed in mouse fallopian tubes. Subsequent immunohistochemical analysis showed that ER␣ was detected in all cell types, whereas ER␤ was mainly localized in ciliated epithelial cells. Second, ER␣ isoform levels were dramatically downregulated in mouse fallopian tubes by treatment with E2 or PPT, an ER␣ agonist, in a time-dependent manner. Third, the presence of ICI 182,780, an ER antagonist, blocked the E2-or PPT-induced downregulation of tubal ER␣ isoform expression in mice. However, alteration of ER␣ immunoreactivity following ICI 182,780 treatment was only detected in epithelial cells of the ampullary region. Fourth, changes in ER␣ isoform expression were found to be coupled to multiple E2 effects on tubal growth, protein synthesis, and secretion in mouse fallopian tube tissues and fluid. In particular, E 2 exhibited positive regulation of IGF-I and IGF-II protein levels. Finally, using growth hormone receptor (GHR) gene-disrupted mice, we showed that regulation by E 2 of IGF production was independent of GHinduced GHR signaling in mouse fallopian tubes in vivo. These data, together with previous studies from our laboratory, suggest that the long-term effects of estrogen agonist promote IGF synthesis and secretion in mouse tubal epithelial cells and fallopian tube fluid via stimulation of ER␣.estrogen receptor-␣ isoforms; tubal epithelial cells A CRITICAL FUNCTION of the mammalian fallopian tube (24) is to provide an optimal microenvironment for the necessary transport and maturation of gametes and the establishment of pregnancy. It excretes the intraluminal tubal fluid, containing a number of growth factors (1,4,29,30,34). Compelling evidence suggests that estrogen may be involved in the regulation of biological processes in the fallopian tube. Studies have shown that ovarian-derived estradiol (E 2 ), the principal bioactive estrogen, is involved in the regulation of tubal protein secretion in human fallopian tubes in vivo (34) and in vitro (63). Insulin-like growth factors (IGFs) have been identified in human and rodent fallopian tube fluid (4). Accumulating data suggest that IGF-I may support embryonic development and survival (1). Moreover, E 2 has been shown to increase endogenous IGF-I mRNA levels in rat fallopian tube and uterus (5, 46), and estrogen-responsive elements are...

show abstract

“…The obtained value of the band intensity of each protein (i.e. MT1, p34Cdk1 or cyclin B) was then normalized by the intensity of b-actin and expressed as relative densitometric units (a ratio of the band intensity of the protein to b-actin for each sample) (Shao et al 2007). MeanGS.E.M.…”

Section: Discussionmentioning

confidence: 99%

Melatonin actions on ovaprim (synthetic GnRH and domperidone)-induced oocyte maturation in carp

Moniruzzaman¹,

Hasan²,

Maitra³

2016

Reproduction

View full text Add to dashboard Cite

The major objective of the present study was to demonstrate the actions of exogenous melatonin on ovaprim (synthetic GnRH and domperidone)-induced final oocyte maturation focusing on the oxidative status of pre-ovulatory follicles in the carp Catla catla. Accordingly, gravid carp during the early spawning phase of the reproductive cycle were injected with melatonin and/or ovaprim at different time intervals or luzindole (a pharmacological blocker of melatonin receptors) before their administration. We studied their effects on the latency period, the rate of germinal vesicle breakdown (GVBD; a visual marker of final oocyte maturation) in oocytes, and the levels of maturation-promoting factor (MPF), as well as oxidative stress, different antioxidants, melatonin and MT1 melatonin receptor protein in the extracts of pre-ovulatory follicles. Notably, melatonin treatment 2 h before the injection of ovaprim resulted in the shortest latency period as well as the highest rate of GVBD and MPF formation. Exogenous melatonin, irrespective of the injection schedule, caused a significant reduction in intra-follicular oxidative stress and an increase in the levels of both enzymatic and nonenzymatic antioxidants, melatonin and its receptor protein. Concentrations of ovarian melatonin in each fish exhibited a significant negative correlation with the level of oxidative stress, but a positive correlation with the rate of GVBD and the activity/level of different antioxidants. However, no significant effects of melatonin and/or ovaprim were detected in luzindole-pretreated carp. Collectively, the present study provides the first evidence that melatonin pretreatment in carp ameliorates ovaprim actions on the process of final oocyte maturation by the formation of MPF and alleviates oxidative stress in pre-ovulatory follicles by stimulating different antioxidants.

show abstract

Ciliated epithelial-specific and regional-specific expression and regulation of the estrogen receptor-β2 in the fallopian tubes of immature rats: a possible mechanism for estrogen-mediated transport process in vivo

Cited by 50 publications

References 75 publications

Sex Hormone–Dependent Regulation of Cilia Beat Frequency in Airway Epithelium

Sex Hormone–Dependent Regulation of Cilia Beat Frequency in Airway Epithelium

Dynamic regulation of estrogen receptor-α isoform expression in the mouse fallopian tube: mechanistic insight into estrogen-dependent production and secretion of insulin-like growth factors

Melatonin actions on ovaprim (synthetic GnRH and domperidone)-induced oocyte maturation in carp

Contact Info

Product

Resources

About