Cinnamon extract and polyphenols affect the expression of tristetraprolin, insulin receptor, and glucose transporter 4 in mouse 3T3-L1 adipocytes

Cao, Heping; Polansky, Marilyn M.; Anderson, Richard A.

doi:10.1016/j.abb.2006.12.034

Cited by 242 publications

(203 citation statements)

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“…2 % HS containing 100 lg/ml HACE (two categories with different concentrations) and three else flasks containing 1 % DMSO as vehicle group. As Cao et al [14] have shown the best results for GLUT4 enhancement in 3T3-L1 under HACE treatment on time exposure of 3 h, we also kept the myotubes in such exposure time status.…”

Section: Affecting Hydro-alcoholic Cinnamon Extract (Hace) On Myotubesmentioning

confidence: 99%

“…Also it is shown that methyl hydroxychalecon, a cinnamon derived component, mimics insulin effects on 3T3-L1 cell line and increases glycogen synthase kinase, glucose uptake and insulin receptor (IR) contents [1]. Other studies have shown GLUT4 enhancement under different concentrations of cinnamon extract or dihydrocinnamic acid (DHH105), a derivative of cinnamon, on GLUT4 content in animal adipose tissue [17] or 3T3-L1 cell line, a good model for studding fat tissue metabolism [14]. But muscular tissue is the most important site for glucose metabolism which uses more than 75 % of body glucose expenditure under insulin impact [18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

“…Most peoples favored to treat their disease by/via a natural agent/way [10,11]; for example for centuries Asian peoples used herbs to treat or prevent diabetes. In this case cinnamon, green tea, turmeric and bitter melon are good examples [12][13][14][15]. Previous studies have shown that 1-6 g/day cinnamon powder intake reduces blood glucose, total cholesterol and LDL-Cholesterol in human [16].…”

Section: Introductionmentioning

confidence: 99%

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Hydro-Alcoholic Cinnamon Extract, Enhances Glucose Transporter Isotype-4 Translocation from Intracellular Compartments into the Cytoplasmic Membrane of C2C12 Myotubes

et al. 2012

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Cinnamon has been used as an anti-diabetic agent for centuries but only in recent few years its mechanism of action has been under investigation. Previous studies showed that cinnamon might exert its anti-diabetic effect via increasing glucose transporter isotype-4 (GLUT4) gene and glycoprotein contents in fat cells. To study if hydro-alcoholic cinnamon extract (HACE) enhances GLUT4 translocation from intracellular compartments of nuclear or endoplasmic reticulum membranes (N/ER) into the cytoplasmic membrane (CM). C2C12 myoblastic cell line were seeded in DMEM plus 20 % FBS and differentiated to myotubes using 2 % horse serum. After myotubes formation, 100 or 1,000 lg/ml HACE, as intervention, and as control 1 % DMSO were added for 3 h. Cells were washed and homogenized followed by ultracentrifuge fractionation, protein separation by SDS-PAGE and GLUT4 detection using semi-quantitative Western blotting. Data analysis was done by two-independent samples t test for comparison of mean ± SD of GLUT4 percent in categories. GLUT4 contents were higher in CM of groups 100 and 1,000 lg/ml HACE and lower in 1 % DMSO treated myotubes (CI = 0.95, P \ 0.05). For N/ER reverse results were obtained (CI = 0.95, P \ 0.05). As our results have shown HACE induces GLUT4 translocation from intra-cell into cell surface. We conclude that cinnamon maybe a choice of type-2 diabetes mellitus treatment because its extract enhances GLUT4 contents in CM where it facilitates glucose entrance into the cell. However it is necessary to trace the signaling pathways which are activated by HACE in muscular tissue.

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Section: Affecting Hydro-alcoholic Cinnamon Extract (Hace) On Myotubesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hydro-Alcoholic Cinnamon Extract, Enhances Glucose Transporter Isotype-4 Translocation from Intracellular Compartments into the Cytoplasmic Membrane of C2C12 Myotubes

et al. 2012

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“…TTP is a very low abundance protein predominantly localized in the cytosol, but its gene expression is rapidly induced by many agents, such as lipopolysaccharide (LPS) [32][33][34][35][36][37], fetal calf serum (FCS) [33], insulin [27,38,39], glucocorticoids [40], zinc [41], cinnamon [42], green tea [43] and herpes simplex virus 1 [44]. Unlike its labile mRNA [27], TTP protein is relatively stable once induced in cells [33].…”

Section: Introductionmentioning

confidence: 99%

Phosphorylation site analysis of the anti-inflammatory and mRNA-destabilizing protein tristetraprolin

Cao¹,

Deterding²,

Blackshear³

2007

Expert Review of Proteomics

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Tristetraprolin (TTP) is a member of the CCCH zinc finger proteins and is an anti-inflammatory protein. Mice deficient in TTP develop a profound inflammatory syndrome with erosive arthritis, autoimmunity and myeloid hyperplasia. TTP binds to mRNA AU-rich elements with high affinity for UUAUUUAUU nucleotides and causes destabilization of those mRNA molecules. TTP is phosphorylated extensively in vivo and is a substrate for multiple protein kinases in vitro. A number of approaches have been used to identify its phosphorylation sites. This article highlights the recent progress and different approaches utilized for the identification of phosphorylation sites in mammalian TTP. Important but limited results are obtained using traditional methods including in vivo labeling, site-directed mutagenesis, phosphopeptide mapping and protein sequencing. Mass spectrometry including MALDI/MS, MALDI/MS/MS, LC/MS/MS, IMAC/MALDI/MS/MS and multidimensional protein identification technology (MudPIT) has led the way in identifying TTP phosphorylation sites. The combination of these approaches has identified multiple phosphorylation sites in mammalian TTP, some of which are predicted by motif scanning to be phosphorylated by several protein kinases. This information should provide the molecular basis for future investigation of TTP's regulatory functions in controlling pro-inflammatory cytokines.

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“…Polyphenols within cinnamon have been identified as upregulators of mouse adipocyte insulin receptors. 4 Peng et al 5 found that polyphenols from cinnamon inhibit the formation of advanced glycation end products in bovine serum albumin. To date, 5 randomized trials studying cinnamon in humans with type 2 diabetes have been published, with conflicting results.…”

mentioning

confidence: 99%

Effectiveness of Cinnamon for Lowering Hemoglobin A1C in Patients with Type 2 Diabetes: A Randomized, Controlled Trial

Crawford¹

2009

The Journal of the American Board of Family Medicine

216

173

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Purpose: Multiple trials in the past have shown conflicting results of whether cinnamon lowers glucose or hemoglobin A1C (HbA1C). The purpose of this study was to determine whether cinnamon lowers HbA1C in patients with type 2 diabetes. I performed a randomized, controlled trial to evaluate whether daily cinnamon plus usual care versus usual care alone lowers HbA1c.Methods: I randomized 109 type 2 diabetics (HbA1C >7.0) from 3 primary care clinics caring for pediatric, adult, and geriatric patients at a United States military base. Participants were randomly allocated to either usual care with management changes by their primary care physician or usual care with management changes plus cinnamon capsules, 1g daily for 90 days. HbA1c was drawn at baseline and 90 days and compared with intention-to-treat analysis. This study was approved by an institutional review board.Results As the worldwide incidence of diabetes increases, the search for dietary adjuncts to treat this lifealtering disease has become far ranging. Cinnamon is purported to be a natural insulin sensitizer, with adverse events of perioral dermatitis and stomatitis reported uncommonly with high intake.1 Both in vitro and in vivo animal studies have shown that cinnamon is an insulin sensitizer. found that polyphenols from cinnamon inhibit the formation of advanced glycation end products in bovine serum albumin. To date, 5 randomized trials studying cinnamon in humans with type 2 diabetes have been published, with conflicting results. 6 -10 The results of these studies are mixed, and it is unclear whether any of them were conducted among primary care populations. Two of these studies showed a possible effect of cinnamon on fasting serum glucose, but they did not examine hemoglobin A1C (HbA1C) levels. One study showed no effect on plasma glucose. Two studies-one each done with patients with type 1 and type 2 diabetes-showed no effect of cinnamon on HbA1C (Table 1). A meta-analysis of these studies in divergent populations measuring different parameters showed no effect of cinnamon on HbA1C, glucose, or lipids. 11This trial strove to replicate the conditions found in primary care, where patients often have medication changes, comorbid conditions, and dietary changes. As such, it was not an efficacy trial with every variable controlled. It attempted to determine whether the intervention of taking cinnamon lowers HbA1C. Effectiveness trials such as this are critical in determining if the interventions This article was externally peer reviewed.

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Cinnamon extract and polyphenols affect the expression of tristetraprolin, insulin receptor, and glucose transporter 4 in mouse 3T3-L1 adipocytes

Cited by 242 publications

References 47 publications

Hydro-Alcoholic Cinnamon Extract, Enhances Glucose Transporter Isotype-4 Translocation from Intracellular Compartments into the Cytoplasmic Membrane of C2C12 Myotubes

Hydro-Alcoholic Cinnamon Extract, Enhances Glucose Transporter Isotype-4 Translocation from Intracellular Compartments into the Cytoplasmic Membrane of C2C12 Myotubes

Phosphorylation site analysis of the anti-inflammatory and mRNA-destabilizing protein tristetraprolin

Effectiveness of Cinnamon for Lowering Hemoglobin A1C in Patients with Type 2 Diabetes: A Randomized, Controlled Trial

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