Circ_0003146 upregulates SCARB1 expression by acting as a miR-1272 sponge to promote malignant behaviors of clear cell renal cell carcinoma

Abstract

Circular RNAs (circRNAs) exhibit essential regulation in the malignant development of clear cell renal cell carcinoma (ccRCC). The aims of this study were to investigate the role and mechanism of circ_0003146 in the biologic behaviors of ccRCC. RNA level analysis was performed through reverse transcription-quantitative PCR assay. Cell proliferation was measured by EdU assay and cell counting kit-8 assay. The protein expression was analyzed using a western blot. Flow cytometry and caspase 3 activity assay were used to assess cell apoptosis. Cell migration and invasion were evaluated via wound healing assay and transwell assay. Circ_0003146 function in vivo was determined by xenograft tumor assay. Dual-luciferase reporter assay was applied for target relation analysis. Circ_0003146 upregulation was detected in ccRCC tissues and cells. Downregulation of circ_0003146 induced inhibition of proliferation, migration, invasion and EMT but the promotion of apoptosis in ccRCC cells. Tumor growth in vivo was inhibited after knockdown of circ_0003146. Circ_0003146 directly interacted with miR-1272, and the miR-1272 sponging effect was responsible for the function of circ_0003146. Scavenger receptor class B type 1 (SCARB1) was a target of miR-1272, and circ_0003146 regulated SCARB1 level by absorbing miR-1272. The regulation of circ_0003146 in ccRCC progression was achieved by upregulating SCARB1 in part. The current findings demonstrated that circ_0003146 contributed to the malignant progression of ccRCC via inducing SCARB1 upregulation by targeting miR-1272.

SCARB1‐encoded circ _0029343 induces p73 splicing to promote growth and metastasis of hepatocellular carcinoma via miR‐486‐5p/SRSF3 axis

“…The effects of SCARB1 encoding circ_0003146 on the development of ccRCC have also been reported. [ 9 ] A recent study has also revealed that circRNA SCARB1 is highly expressed in HCC and accelerates HCC cell proliferation and migration. [ 30 ] It is known that circRNAs play a key role in the occurrence and development of tumors, including HCC.…”

“…A previous study has indicated that SCARB1 can encode circular RNA_0003146 (circ_0003146) to affect the malignant development of clear cell renal cell carcinoma (ccRCC). [ 9 ] Circular RNAs (circRNAs), a certain class of noncoding RNAs secreted by backspacing, have attracted great attention. [ 10 ] Multiple circRNAs regulate cell biological processes in HCC progression.…”

Multi-omics analysis reveals genomic, clinical and immunological features of SARS-CoV-2 virus target genes in pan-cancer

“…TMPRSS2, a key gene mediating the entry of SARS-CoV-2 into humans, is highly expressed in prostate cancer and its high expression could promote the development of prostate cancer (37)(38)(39)(40). NRP1, serving as a key factor of SARS-CoV-2 virus infection (41), could increase the susceptibility of cancer patients to SARS-CoV-2 virus and targeting (42), and its overexpression is associated with poor prognosis in bladder cancer cells and hepatocellular carcinoma, and inhibiting its expression could promote tumor cell apoptosis (43,44). In addition, the increased expression of SCARB1 could promote cell transformation towards malignancy of clear cell renal cell carcinoma leading to poor prognosis of patients (45), while the over-expression of AXL could accelerate EGFR mutation and promote the metastasis, invasion or drug resistance of tumors such as lung cancer (46), colorectal cancer (47), pancreatic cancer (48) and prostate tumor (49).…”