CircLPAR3 Acts as an Oncogene in Oral Squamous Cell Carcinoma Through Regulating the miR-643/HMGB2 Network

Fu, Yanjun; Qiu, Chunyan; Yang, Yanjun; Lü, Jian; Qi, Yun

doi:10.1007/s10528-021-10134-y

Cited by 5 publications

(8 citation statements)

References 28 publications

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“…suggested that circLPAR3 was overexpressed in OSCC samples and cell lines, and circLPAR3 silencing decreased xenograft tumor growth and facilitated cell apoptosis, restrained cell proliferation, stemness, and migration through repressing HMGB2 by liberating miR-643. 19 Consistent with the results of Fu et al, our data exhibited that circLPAR3 had higher levels in OSCC samples and cell lines, and circLPAR3 downregulation reduced xenograft tumor growth in vivo and induced cell apoptosis, repressed cell proliferation, and reduced migration in OSCC cells in vitro. In addition, we also discovered that circLPAR3 could distinguish between OSCC samples and normal samples, and OSCC patients with high circLPAR3 expression had a worse prognosis.…”

Section: Discussionsupporting

confidence: 92%

“…Cheng et al indicated that circLPAR3 sponged miR‐433/miR‐375 and elevated HMGB1 expression, resulting in ESCC growth 24 . Fu et al suggested that circLPAR3 was overexpressed in OSCC samples and cell lines, and circLPAR3 silencing decreased xenograft tumor growth and facilitated cell apoptosis, restrained cell proliferation, stemness, and migration through repressing HMGB2 by liberating miR‐643 19 . Consistent with the results of Fu et al, our data exhibited that circLPAR3 had higher levels in OSCC samples and cell lines, and circLPAR3 downregulation reduced xenograft tumor growth in vivo and induced cell apoptosis, repressed cell proliferation, and reduced migration in OSCC cells in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…Hsa_circ_0004390 (circLPAR3), located at chr1: 85331067–85,331,821, is overexpressed in the circRNA expression profile in OSCC samples 18 . A recent report suggested the oncogenic role of circLPAR3 in OSCC, 19 but the role of circLPAR3 and its function as a miRNA sponge needs to be further explored.…”

Section: Introductionmentioning

confidence: 99%

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Downregulation of circLPAR3 inhibits tumor progression and glycolysis by liberating miR‐144‐3p and upregulating LPCAT1 in oral squamous cell carcinoma

Pan

Xie

et al. 2022

Laryngoscope Investig Oto

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Background: Increasing evidence demonstrated the important roles of circular RNAs (circRNAs) in human cancer progression, including oral squamous cell carcinoma (OSCC). The study intentions were to explore the role and molecular mechanism of hsa_circ_0004390 (circLPAR3) in OSCC progression.Methods: Expression of circLPAR3 in collected samples and cultured cell lines was detected with real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR). Loss-of-function experiments were performed to determine the effect of circLPAR3 silencing on OSCC cell proliferation, migration, invasion, apoptosis, angiopoiesis, and glycolysis. The sponge function of circLPAR3 was predicted by bioinformatics analysis and validated by the dual-luciferase reporter and RNA pull-down assays. In vivo experiments were conducted to validate the function of circLPAR3.Results: A marked increase in circLPAR3 expression was observed in OSCC samples and cell lines. Furthermore, circLPAR3 could distinguish OSCC samples from paired non-tumor samples, and patients with high circLPAR3 expression had a poor prognosis. Furthermore, circLPAR3 inhibition decreased OSCC growth in xenograft mouse models. Moreover, circLPAR3 silencing repressed cell proliferation, migration, invasion, angiopoiesis, glycolysis, and induced cell apoptosis in OSCC cells in vitro.Mechanically, circLPAR3 sponged miR-144-3p to prohibit the inhibiting effect of miR-144-3p on LPCAT1, thus promoting OSCC progression. Conclusion:CircLPAR3 exerted a tumor-promoting effect on OSCC growth through elevating LPCAT1 expression via functioning as a miR-144-3p sponge. This study supports the possible role of circLPAR3 in the diagnosis, prognosis, and treatment of OSCC.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Downregulation of circLPAR3 inhibits tumor progression and glycolysis by liberating miR‐144‐3p and upregulating LPCAT1 in oral squamous cell carcinoma

Pan

Xie

et al. 2022

Laryngoscope Investig Oto

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“…22,23 MiR-643 has been reported functioned as potential tumor suppressor in many cancers. 24,25 Wu et al showed that miR-643 directly target TXNDC9, inhibiting gastric cancer cells viability, invasion, and migration. 25 However, another study indicated that miR-643 promotes proliferation and inhibits apoptosis of papillary thyroid carcinoma cells.…”

Section: Discussionmentioning

confidence: 99%

“…MiRNA performed vital functions in regulating the radiosensitivity of tumor cells 22,23 . MiR‐643 has been reported functioned as potential tumor suppressor in many cancers 24,25 . Wu et al showed that miR‐643 directly target TXNDC9, inhibiting gastric cancer cells viability, invasion, and migration 25 .…”

Section: Discussionmentioning

confidence: 99%

miR‐643 suppresses cell invasion and radioresistant of lung cancer through RAF1

Tian

Ding

WANG

et al. 2023

Precision Medical Sciences

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RAF1 (c‐raf) has been known as an important tumor‐promoter in many cancers. However, the regulatory mechanisms affecting RAF1 expressions are rarely reported. This study aimed to predict the candidate miRNAs of RAF1 gene and verify their functions in the progression of lung cancer. The expression of miR‐643 was detected by reverse transcription polymerase chain reaction (RT‐PCR). A dual‐luciferase report system was used to verify the relationship between miR‐643 and RAF1. RT‐PCR and Western blot were used to analyze the regulatory relationship between miR‐643 and RAF1. Transwell chamber, scratch, and monoclonal tests showed that miR‐643 affected the proliferation, migration, and radiosensitivity of H1299 and A549 cells by targeting the RAF1 gene. The expression of miR‐643 in lung cancer cells was lower than that in the bronchial epithelioid cells (CRL‐2741), and luciferase reporter experiment confirmed that miR‐643 targeted RAF1. MiR‐643 overexpression decreased the RAF1 expression, thereby decreasing cell migration, proliferation, and radiation resistance through the AKT/nuclear factor‐κB pathway. miR‐643 in lung cancer cells could inhibit cell proliferation, invasion, and metastasis and increase the radiosensitivity by downregulating RAF1.

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The endogenous association among MMP2/miR-1248/Circ_0087558/miR-643/ MAP2K6 axis can contribute to brain metastasis in basal-like subtype of breast cancer

Khoshbakht,

Zomorodi Anbaji,

Darzi

et al. 2024

Heliyon

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CircLPAR3 Acts as an Oncogene in Oral Squamous Cell Carcinoma Through Regulating the miR-643/HMGB2 Network

Cited by 5 publications

References 28 publications

Downregulation of circLPAR3 inhibits tumor progression and glycolysis by liberating miR‐144‐3p and upregulating LPCAT1 in oral squamous cell carcinoma

Downregulation of circLPAR3 inhibits tumor progression and glycolysis by liberating miR‐144‐3p and upregulating LPCAT1 in oral squamous cell carcinoma

miR‐643 suppresses cell invasion and radioresistant of lung cancer through RAF1

The endogenous association among MMP2/miR-1248/Circ_0087558/miR-643/ MAP2K6 axis can contribute to brain metastasis in basal-like subtype of breast cancer

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