CircPRMT5 circular RNA promotes proliferation of colorectal cancer through sponging miR‐377 to induce E2F3 expression

Yang, Bairen; Du, Ke; Yang, Chuanhua; Xiang, Lili; Xü, Ying; Cao, Chen; Zhang, Junhui; Liu, Wenneng

doi:10.1111/jcmm.15019

Cited by 34 publications

(23 citation statements)

References 29 publications

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“…20 CircPRMT5 is also demonstrated to inhibit miR-30c and miR-377. 12,13 In our study, we also validated that miR-30c was sponged by circPRMT5. We demonstrated their direct interaction through luciferase reporter assay and RIP assay.…”

Section: Discussionsupporting

confidence: 71%

“…17 CircPRMT5 was involved in bladder cancer and colon cancer. 12,13 However, whether circPRMT5 regulates PTC needs investigation. In this work, we found that circPRMT5 was upregulated in PTC tissues and cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…12 Besides, circPRMT5 also increases growth of colorectal cancer cells. 13 Whether circPRMT5 exerts a role in PTC remains elusive. In this study, we found that circPRMT5 was upregulated in PTC tissues and circPRMT5 knockdown inhibited proliferation and metastasis of PTC cells.…”

Section: Introductionmentioning

confidence: 99%

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<p>Circular RNA CircPRMT5 Accelerates Proliferation and Invasion of Papillary Thyroid Cancer Through Regulation of miR-30c/E2F3 Axis</p>

Xue

Cheng

et al. 2020

CMAR

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Background: The role of circular RNA (circRNA) in papillary thyroid cancer (PTC) is largely unknown. This study aims to determine the function and mechanism of circPRMT5 in the regulation of PTC development. Methods: PTC tissues and cell lines were used to determine circPRMT5 expression via quantitative real-time polymerase chain reaction. Small interfering RNA (siRNA) was utilized to knock down circPRMT5. Proliferation was analyzed through CCK8 and colony formation assays. Transwell assay was performed to determine cell migration and invasion. Luciferase assay and RIP assay were carried out to analyze the interaction between circPRMT5 and miR-30c. Results: CircPRMT5 expression was upregulated in PTC tissues and cell lines. And circPRMT5 level was positively linked with advanced stage and lymph node metastasis. CircPRMT5 knockdown inhibited proliferation, migration and invasion while inducing apoptosis. CircPRMT5 worked as a competing endogenous RNA for miR-30c. By inhibiting miR-30c, circPRMT5 promoted the expression of E2F3. Conclusion: Our findings demonstrate that circPRMT5 acts as an oncogenic circRNA to promote PTC progression via regulating miR-30c/E2F3 axis.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

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<p>Circular RNA CircPRMT5 Accelerates Proliferation and Invasion of Papillary Thyroid Cancer Through Regulation of miR-30c/E2F3 Axis</p>

Xue

Cheng

et al. 2020

CMAR

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“…1b). Previous study has indicated that E2F3 was highly expressed and contributed to tumor progression in a variety of tumors, such as colorectal [19,20], melanoma [21][22][23][24][25] and bladder cancer [26][27][28][29]. We then (See figure on previous page.)…”

Section: Resultsmentioning

confidence: 93%

A novel LncRNA transcript, RBAT1, accelerates tumorigenesis through interacting with HNRNPL and cis-activating E2F3

Chai

et al. 2020

Mol Cancer

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Background: Long non-coding RNAs (lncRNAs) have been identified as important epigenetic regulators that play critical roles in human cancers. However, the regulatory functions of lncRNAs in tumorigenesis remains to be elucidated. Here, we aimed to investigate the molecular mechanisms and potential clinical application of a novel lncRNA, retinoblastoma associated transcript-1 (RBAT1), in tumorigenesis. Methods: RBAT1 expression was determined by real-time PCR in both retinoblastoma (Rb) and bladder cancer (BCa) cell lines and clinical tissues. Chromatin isolation using RNA purification (ChIRP) assays were performed to identify RBAT1-interacting proteins. Patient-derived xenograft (PDX) retinoblastoma models were established to test the therapeutic potential of RBAT1-targeting GapmeRs. Results: Here, we found that RBAT1 expression was significantly higher in Rb and BCa tissues than that in adjacent tissues. Functional assays revealed that RBAT1 accelerated tumorigenesis both in vitro and in vivo. Mechanistically, RBAT1 recruited HNRNPL protein to E2F3 promoter, thereby activating E2F3 transcription. Therapeutically, GapmeRmediated RBAT1 silencing significantly inhibited tumorigenesis in orthotopic xenograft retinoblastoma models derived from Rb cell lines and Rb primary cells. Conclusions: RBAT1 overexpression upregulates a known oncogene, E2F3, via directly recruiting HNPNPL to its promoter and cis-activating its expression. Our finding provides a novel mechanism of lncRNA biology and provides potential targets for diagnosis and treatment of Rb and BCa.

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“…Both in vitro and in vivo experiments, Zhang demonstrated that high levels of miR-34a expression could inhibit the expression of direct target gene E2F3, and then inhibit the growth of CRC cells [29]. CircPRMT5 is an upstream regulator of E2F3 and it could promote the proliferation of CRC by inducing the expression of E2F3 [30]. Comparing with other E2F members, E2F4 has been reported in a lower extent.…”

Section: Discussionmentioning

confidence: 99%

A Research of the Predictive Values of E2F Factors in Colorectal Cancer

Zhang¹,

Yang²,

Zhang³

et al. 2020

Preprint

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Background: E2F family of transcription factors are related to cell cycle and apoptosis with activate or repress function on human cancer. Their critical role in colorectal cancer (CRC), including colon adenocarcinoma (COAD) and rectum adenocarcinoma (READ), were widely investigated with contradictory and ambiguous results. Methods: In this study, we explored the expressions of these eight E2F genes and their predictive values on the overall survive (OS) and disease-free survival (DFS) in CRC patients by means of bioinformatic databases, such as ONCOMINE, GEPIA, DAVIA and cBioPortal.Results: We found that the transcriptional levels of E2F1/2/3/5/7/8 were significantly higher in CRC patients than that in normal samples, while the E2F4/6 showed no significantly difference from GEPIA database. The transcriptional levels of all eight E2F factors showed no correlation with four tumor stages, except for the E2F3 is significantly varied in COAD patients. Survival analysis based on GEPIA database indicated that lower E2F3 and E2F4 mRNA expressions were significantly associated with the OS of the COAD patients.Conclusions: This study manifested that the E2F1/2/3/5/7/8 are potential biomarkers of CRC patients, and E2F3 could be used as the predictive factor of tumor stage in COAD patients. E2F3 and E2F4 might be the prognostic markers for COAD patients.

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CircPRMT5 circular RNA promotes proliferation of colorectal cancer through sponging miR‐377 to induce E2F3 expression

Cited by 34 publications

References 29 publications

<p>Circular RNA CircPRMT5 Accelerates Proliferation and Invasion of Papillary Thyroid Cancer Through Regulation of miR-30c/E2F3 Axis</p>

<p>Circular RNA CircPRMT5 Accelerates Proliferation and Invasion of Papillary Thyroid Cancer Through Regulation of miR-30c/E2F3 Axis</p>

A novel LncRNA transcript, RBAT1, accelerates tumorigenesis through interacting with HNRNPL and cis-activating E2F3

A Research of the Predictive Values of E2F Factors in Colorectal Cancer

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