CircRNA circ-ITCH suppresses papillary thyroid cancer progression through miR-22-3p/CBL/β-catenin pathway

Wang, Minnan; Chen, Bin; Ru, Zixuan; Li, Cong

doi:10.1016/j.bbrc.2018.08.175

Cited by 132 publications

(102 citation statements)

References 19 publications

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“…13 Also, the cellular impact of circ-ITCH has been observed in papillary thyroid cancer cells, whose proliferation is inhibited but apoptosis is enhanced by circ-ITCH. 30 Moreover, it is also revealed that circ-ITCH influence diverse cell activities by mediating multiple genes or signaling pathways. For example, circ-ITCH acts as a tumor suppressor through sponging miR-124 and enhancing linear ITCH expression in glioma, and another study reveals that circ-ITCH inhibits disease progression through sponging miR-7 and miR-24 in bladder cancer.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of Circular RNA Itchy E3 Ubiquitin Protein Ligase Inhibits Cell Proliferation and Promotes Cell Apoptosis Through Targeting MiR-197 in Prostate Cancer

Yuan

Chen

Huang

2019

Technol Cancer Res Treat

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Objective: This study aimed to investigate the effect of circular RNA itchy E3 ubiquitin protein ligase on cell proliferation and apoptosis and to explore its target micro-RNAs in prostate cancer cells. Methods: Circular RNA itchy E3 ubiquitin protein ligase expression in human prostate cancer cells and normal prostate epithelial cells was determined by real time-quantitative polymerase chain reaction assay. Circular RNA itchy E3 ubiquitin protein ligase overexpression plasmids (circular RNA itchy E3 ubiquitin protein ligase(+) group and control overexpression plasmids group were transfected with PC-3 cells. Rescue experiment was performed by transfection of circular RNA itchy E3 ubiquitin protein ligase overexpression and micro-197 overexpression plasmids (circular RNA itchy E3 ubiquitin protein ligase overexpression plasmids/micro RNA (+) group) into PC-3 cells. Cell Counting Kit-8 and annexin V/propidium iodide assays were conducted to evaluate cell proliferation and apoptosis, respectively. Western blot was performed to determine the expressions of apoptotic-related markers. Results: Circular RNA itchy E3 ubiquitin protein ligase expression was decreased in DU 145, 22RV1, VCaP, and PC-3 cells compared to RWPE cells. In PC-3 cells, cell proliferation rate was reduced in circular RNA itchy E3 ubiquitin protein ligase overexpression plasmids group compared to control overexpression plasmids group at 48 hours and 72 hours. Cell apoptosis rate was elevated in circular RNA itchy E3 ubiquitin protein ligase overexpression plasmids group compared to control overexpression plasmids group at 48 hours, and Western blot showed the similar results. Micro RNA-197 but not micro RNA-31 or micro RNA-432 was the target micro-RNA of circular RNA itchy E3 ubiquitin protein ligase. In rescue experiments, cell proliferation rate was elevated, but apoptosis rate was reduced in circular RNA itchy E3 ubiquitin protein ligase overexpression plasmids/micro RNA (+) group compared to circular RNA itchy E3 ubiquitin protein ligase overexpression plasmids group, indicating that circular RNA itchy E3 ubiquitin protein ligase upregulation inhibited cell proliferation but promoted apoptosis through downregulating micro RNA-197. Conclusion: Circular RNA itchy E3 ubiquitin protein ligase upregulation suppresses cell proliferation but promotes apoptosis through targeting micro RNA-197 in prostate cancer. Our study may provide a new insight for the treatment of prostate cancer.

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Section: Discussionmentioning

confidence: 99%

Upregulation of Circular RNA Itchy E3 Ubiquitin Protein Ligase Inhibits Cell Proliferation and Promotes Cell Apoptosis Through Targeting MiR-197 in Prostate Cancer

Yuan

Chen

Huang

2019

Technol Cancer Res Treat

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“…22 More particularly, previous studies have indicated circRNAs might play essential roles in tumorigenesis, progression, prognosis and treatment of PTC, such as circRNA-102171, circ-ITCH, circZFR and circ-0137287. 7,8,[23][24][25] However, the role of circPVT1 in PTC and its mechanism remain largely unknown. In this study, we rst elucidated the oncogenic role of circPVT1 in PTC and explored the interaction between circPVT1 and miR-126 in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of circPVT1 inhibits progression of papillary thyroid carcinoma by sponging miR-126

Tao

Tian

et al. 2019

RSC Adv.

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“…This led to suppress activation of the Wnt/β-catenin pathway and consequently inhibited the progression of PTC. 176 Moreover, circZFR was negative correlated with clinical severity of PTC patients.…”

Section: Papillary Thyroid Carcinomamentioning

confidence: 96%

<p>Biological Roles and Mechanisms of Circular RNA in Human Cancers</p>

Tang

Hann

2020

OTT

136

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Circular RNA (circRNA) is an intriguing class of RNA with covalently closedloop structure and is highly stable and conservative. As new members of the ncRNAs, the function, mechanism, potential diagnostic biomarker, and therapeutic target have raised increased attention. Most circRNAs are presented with characteristics of abundance, stability, conservatism, and often exhibiting tissue/developmental-stage-specific manner. Over 30,000 circRNAs have been identified with their unique structures to maintain stability more easily than linear RNAs. An increased numbers of circRNAs are dysregulated and involved in several biological processes of malignance, such as tumorigenesis, growth, invasion, metastasis, apoptosis, and vascularization. Emerging evidence suggests that circRNAs play important roles by acting as miRNA sponge or protein scaffolding, autophagy regulators, and interacting with RNA-binding protein (RBP), which may potentially serve as a novel promising biomarker for prevention, diagnosis and therapeutic target for treatment of human cancer with great significance either in scientific research or clinic arena. This review introduces concept, major features of circRNAs, and mainly describes the major biological functions and clinical relevance of circRNAs, as well as expressions and regulatory mechanisms in various types of human cancer, including pathogenesis, mode of action, potential target, signaling regulatory pathways, drug resistance, and therapeutic biomarkers. All of which provide evidence for the potential utilities of circRNAs in the diagnosis and treatment of cancer.

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CircRNA circ-ITCH suppresses papillary thyroid cancer progression through miR-22-3p/CBL/β-catenin pathway

Cited by 132 publications

References 19 publications

Upregulation of Circular RNA Itchy E3 Ubiquitin Protein Ligase Inhibits Cell Proliferation and Promotes Cell Apoptosis Through Targeting MiR-197 in Prostate Cancer

Upregulation of Circular RNA Itchy E3 Ubiquitin Protein Ligase Inhibits Cell Proliferation and Promotes Cell Apoptosis Through Targeting MiR-197 in Prostate Cancer

Knockdown of circPVT1 inhibits progression of papillary thyroid carcinoma by sponging miR-126

<p>Biological Roles and Mechanisms of Circular RNA in Human Cancers</p>

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